What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry

Targeted proteomics has gained significant popularity in mass spectrometry-based protein quantification as a method to detect proteins of interest with high sensitivity, quantitative accuracy and reproducibility. However, with the emergence of a wide variety of targeted proteomics methods, some of t...

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Detalles Bibliográficos
Autores: Borràs, Eva, Sabidó Aguadé, Eduard, 1981-
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/34896
Acceso en línea:http://hdl.handle.net/10230/34896
http://dx.doi.org/10.1002/pmic.201700180
Access Level:acceso abierto
Palabra clave:Data independent acquisition
Parallel reaction monitoring
Mass spectrometry
Targeted proteomics
Selected reaction monitoring
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spelling What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometryBorràs, EvaSabidó Aguadé, Eduard, 1981-Data independent acquisitionParallel reaction monitoringMass spectrometryTargeted proteomicsSelected reaction monitoringTargeted proteomics has gained significant popularity in mass spectrometry-based protein quantification as a method to detect proteins of interest with high sensitivity, quantitative accuracy and reproducibility. However, with the emergence of a wide variety of targeted proteomics methods, some of them with high-throughput capabilities, it is easy to overlook the essence of each method and to determine what makes each of them a targeted proteomics method. In this viewpoint, we revisit the main targeted proteomics methods and classify them in four categories differentiating those methods that perform targeted data acquisition from targeted data analysis, and those methods that are based on peptide ion data (MS1 targeted methods) from those that rely on the peptide fragments (MS2 targeted methods).Wiley20182017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/34896http://dx.doi.org/10.1002/pmic.201700180reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésProteomics. 2017 Sep;17(17-18):1700180This is the peer reviewed version of the following article: Borràs E, Sabidó E. What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry. Proteomics. 2017 Sep;17(17-18):1700180, which has been published in final form at http://dx.doi.org/10.1002/pmic.201700180. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.info:eu-repo/semantics/openAccessoai:recercat.cat:10230/348962026-05-29T05:05:01Z
dc.title.none.fl_str_mv What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
title What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
spellingShingle What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
Borràs, Eva
Data independent acquisition
Parallel reaction monitoring
Mass spectrometry
Targeted proteomics
Selected reaction monitoring
title_short What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
title_full What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
title_fullStr What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
title_full_unstemmed What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
title_sort What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry
dc.creator.none.fl_str_mv Borràs, Eva
Sabidó Aguadé, Eduard, 1981-
author Borràs, Eva
author_facet Borràs, Eva
Sabidó Aguadé, Eduard, 1981-
author_role author
author2 Sabidó Aguadé, Eduard, 1981-
author2_role author
dc.subject.none.fl_str_mv Data independent acquisition
Parallel reaction monitoring
Mass spectrometry
Targeted proteomics
Selected reaction monitoring
topic Data independent acquisition
Parallel reaction monitoring
Mass spectrometry
Targeted proteomics
Selected reaction monitoring
description Targeted proteomics has gained significant popularity in mass spectrometry-based protein quantification as a method to detect proteins of interest with high sensitivity, quantitative accuracy and reproducibility. However, with the emergence of a wide variety of targeted proteomics methods, some of them with high-throughput capabilities, it is easy to overlook the essence of each method and to determine what makes each of them a targeted proteomics method. In this viewpoint, we revisit the main targeted proteomics methods and classify them in four categories differentiating those methods that perform targeted data acquisition from targeted data analysis, and those methods that are based on peptide ion data (MS1 targeted methods) from those that rely on the peptide fragments (MS2 targeted methods).
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/34896
http://dx.doi.org/10.1002/pmic.201700180
url http://hdl.handle.net/10230/34896
http://dx.doi.org/10.1002/pmic.201700180
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Proteomics. 2017 Sep;17(17-18):1700180
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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