Bacteriospermia and porcine reproduction: effects on sperm quality and fertility potential of boar spermatozoa

Boar semen is not collected under sterile conditions,so boar ejaculates are commonly contaminated with a broad range of microorganisms, which may affect sperm quality and lead to a low reproductive performance and severe economic losses in the swine industry. For that reason, the present thesis has...

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Detalles Bibliográficos
Autor: Sepúlveda, Lilian
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/395943
Acceso en línea:http://hdl.handle.net/10803/395943
Access Level:acceso abierto
Palabra clave:Pseudomonas aeruginosa
Clostridium perfringens
Sperm quality
Qualitat espermàtica
Calidad espermática
Boar spermatozoa
Espermatozoides de porcí
Espermatozoides de porcino
Pig-male reproductor
Mascle reproductor porcí
Macho reproductor porcino
Sus domesticus
Sperm capacitation
Capacitació espermàtica
Capacitación espermática
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Descripción
Sumario:Boar semen is not collected under sterile conditions,so boar ejaculates are commonly contaminated with a broad range of microorganisms, which may affect sperm quality and lead to a low reproductive performance and severe economic losses in the swine industry. For that reason, the present thesis has focused on the effects that different concentrations of two different bacteria cause upon sperm quality and fertility potential of boar spermatozoa. Concretely, the present study has established that concentrations over 10e6-10e7 CFU/mL of Pseudomonas aeruginosa and Clostridium perfringens cause detrimental effects on sperm motility, sperm viability and sperm acrosome integrity. Moreover, concentrations of P. aeruginosa higher than 10e4-10e6 CFU/mL also affect the ability of boar sperm to become fully capacitated, an essential requirement for a proper fertilisation. In conclusion, this thesis has established a concentration threshold from which sperm quality and fertility potential are affected.