Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation

Stemcells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate...

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Autores: Maymó, Julieta L., Riedel, Rodrigo, Pérez Pérez, Antonio, Magatti, Marta, Maskin, Bernardo, Dueñas Díez, José Luis, Parolini, Ornella, Sánchez Margalet, Víctor, Varone, Cecilia L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/177047
Acceso en línea:https://hdl.handle.net/11441/177047
https://doi.org/10.1371/journal.pone.0191489
Access Level:acceso abierto
Palabra clave:human amniotic epithelial cells
Cells
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spelling Proliferation and survival of human amniotic epithelial cells during their hepatic differentiationMaymó, Julieta L.Riedel, RodrigoPérez Pérez, AntonioMagatti, MartaMaskin, BernardoDueñas Díez, José LuisParolini, OrnellaSánchez Margalet, VíctorVarone, Cecilia L.human amniotic epithelial cellsCellsStemcells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate toward all three germ layers, they are not tumorigenic and they have immunosuppressive properties. Although liver transplantation is the best way to treat acute and chronic hepatic failure patients, there are several obstacles. Recently, stem cells have beenspotlighted as alternative source of hepatocytes because of their potential for hepatogenic differentiation. In this work, we aimed to study the proliferation and survival of the hAECsduring their hepatic differentiation. We have also analyzed the changes in pluri potency and hepatic markers. We differentiated amniotic cells applying a specific hepatic differentiation (HD) protocol. We determined by qRT-PCR that hAECs express significant levels of SOX-2, OCT-4 and NANOGduringatleast 15daysinculture and these pluripotent markers diminish during HD. SSEA-4 expression was reduced during HD, measured by immunofluorescence. Morphological characteristics became more similar to hepatic ones in differentiated cells and representative hepatic markers significantly augmented their expres sion, measured by qRT-PCR andWesternblot.Cells achieved a differentiation efficiency of 75%.WeobservedthatHDinducedproliferation and promoted survival of hAECs, during 30 days in culture, evaluated by 3 H-thymidine incorporation and MTT assay. HD also promoted changes in hAECscell cycle. Cyclin D1 expression increased, while p21 and p53 levels were reduced. Immunofluorescence analysis showed that Ki-67 expression was upregu lated during HD. Finally, ERK 1/2 phosphorylation, which is intimately linked to proliferation andcell survival, augmented during all HD process and the inhibition of this signaling path wayaffected not only proliferation but also differentiation. Our results suggest that HD promotes proliferation and survival of hAECs, providing important evidence about the mech anisms governing their hepatic differentiation. We bring new knowledge concerning some of the optimal transplantation conditions for these hepatic like cells.Public Library of Science (PLoS)Bioquímica Médica y Biología Molecular e InmunologíaCirugíaCTS151: Bioquímica MedicaCTS607: Salud Reproductiva de la Mujer2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/177047https://doi.org/10.1371/journal.pone.0191489reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlos One, 13 (1), e0191489.https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191489info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1770472026-06-17T12:51:07Z
dc.title.none.fl_str_mv Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
title Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
spellingShingle Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
Maymó, Julieta L.
human amniotic epithelial cells
Cells
title_short Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
title_full Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
title_fullStr Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
title_full_unstemmed Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
title_sort Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
dc.creator.none.fl_str_mv Maymó, Julieta L.
Riedel, Rodrigo
Pérez Pérez, Antonio
Magatti, Marta
Maskin, Bernardo
Dueñas Díez, José Luis
Parolini, Ornella
Sánchez Margalet, Víctor
Varone, Cecilia L.
author Maymó, Julieta L.
author_facet Maymó, Julieta L.
Riedel, Rodrigo
Pérez Pérez, Antonio
Magatti, Marta
Maskin, Bernardo
Dueñas Díez, José Luis
Parolini, Ornella
Sánchez Margalet, Víctor
Varone, Cecilia L.
author_role author
author2 Riedel, Rodrigo
Pérez Pérez, Antonio
Magatti, Marta
Maskin, Bernardo
Dueñas Díez, José Luis
Parolini, Ornella
Sánchez Margalet, Víctor
Varone, Cecilia L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Médica y Biología Molecular e Inmunología
Cirugía
CTS151: Bioquímica Medica
CTS607: Salud Reproductiva de la Mujer
dc.subject.none.fl_str_mv human amniotic epithelial cells
Cells
topic human amniotic epithelial cells
Cells
description Stemcells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate toward all three germ layers, they are not tumorigenic and they have immunosuppressive properties. Although liver transplantation is the best way to treat acute and chronic hepatic failure patients, there are several obstacles. Recently, stem cells have beenspotlighted as alternative source of hepatocytes because of their potential for hepatogenic differentiation. In this work, we aimed to study the proliferation and survival of the hAECsduring their hepatic differentiation. We have also analyzed the changes in pluri potency and hepatic markers. We differentiated amniotic cells applying a specific hepatic differentiation (HD) protocol. We determined by qRT-PCR that hAECs express significant levels of SOX-2, OCT-4 and NANOGduringatleast 15daysinculture and these pluripotent markers diminish during HD. SSEA-4 expression was reduced during HD, measured by immunofluorescence. Morphological characteristics became more similar to hepatic ones in differentiated cells and representative hepatic markers significantly augmented their expres sion, measured by qRT-PCR andWesternblot.Cells achieved a differentiation efficiency of 75%.WeobservedthatHDinducedproliferation and promoted survival of hAECs, during 30 days in culture, evaluated by 3 H-thymidine incorporation and MTT assay. HD also promoted changes in hAECscell cycle. Cyclin D1 expression increased, while p21 and p53 levels were reduced. Immunofluorescence analysis showed that Ki-67 expression was upregu lated during HD. Finally, ERK 1/2 phosphorylation, which is intimately linked to proliferation andcell survival, augmented during all HD process and the inhibition of this signaling path wayaffected not only proliferation but also differentiation. Our results suggest that HD promotes proliferation and survival of hAECs, providing important evidence about the mech anisms governing their hepatic differentiation. We bring new knowledge concerning some of the optimal transplantation conditions for these hepatic like cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/177047
https://doi.org/10.1371/journal.pone.0191489
url https://hdl.handle.net/11441/177047
https://doi.org/10.1371/journal.pone.0191489
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plos One, 13 (1), e0191489.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191489
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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