Kinetic and microbiological characterization of aerobic granules performing partial nitritation of a low-strength wastewater at 10ºC

A granular airlift reactor enriched in ammonia oxidizing bacteria (AOB) was operated at 10 °C performing stable partial nitritation in the long-term. The reactor treated a synthetic low-strength influent during 250 days with an average nitrogen loading rate of 0.63 ± 0.06 g N L⁻¹ d⁻¹. Nitrate produc...

Descripción completa

Detalles Bibliográficos
Autores: Reino, Clara|||0000-0003-3378-3542, Suárez Ojeda, María Eugenia|||0000-0003-2520-2701, Pérez Cañestro, Julio|||0000-0002-0119-5570, Carrera, Julian|||0000-0002-2599-2312
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:166267
Acceso en línea:https://ddd.uab.cat/record/166267
https://dx.doi.org/urn:doi:10.1016/j.watres.2016.05.059
Access Level:acceso abierto
Palabra clave:Partial nitritation
Mainstream
Low temperature
NOB-Repression
AOB-Enrichment
Anammox
Descripción
Sumario:A granular airlift reactor enriched in ammonia oxidizing bacteria (AOB) was operated at 10 °C performing stable partial nitritation in the long-term. The reactor treated a synthetic low-strength influent during 250 days with an average nitrogen loading rate of 0.63 ± 0.06 g N L⁻¹ d⁻¹. Nitrate production was barely detected, being the average concentration in the effluent of 0.6 ± 0.3 mg N-NO3 L⁻¹. Furthermore, a suitable effluent for a subsequent reactor performing the anammox process was achieved. A maximum specific growth rate as high as 0.63 ± 0.05 d⁻¹ was determined by performing kinetic experiments with the granular sludge in a chemostat and fitting the results to the Monod model. Pyrosequencing analysis showed a high enrichment in AOB (41 and 65% of the population were identified as Nitrosomonas genus on day 98 and 233, respectively) and an effective repression of nitrite oxidizing bacteria in the long-term. Pyrosequencing analysis also identified the coexistence of nitrifying bacteria and heterotrophic psychrotolerant microorganisms in the granular sludge. Some psychrotolerant microorganisms are producers of cryoprotective extracellular polymeric substances that could explain the better survival of the whole consortia at cold temperatures.