Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip

20 p.-6 fig.

Detalles Bibliográficos
Autores: Villacampa, Alicia, Sora, Ludovico, Herranz, Raúl, Medina, F. Javier, Ciska, Malgorzata
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/259722
Acceso en línea:http://hdl.handle.net/10261/259722
Access Level:acceso abierto
Palabra clave:Clinostat
Gravitropism
Gravity perception
Microgravity simulation
Plant
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spelling Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tipVillacampa, AliciaSora, LudovicoHerranz, RaúlMedina, F. JavierCiska, MalgorzataClinostatGravitropismGravity perceptionMicrogravity simulationPlant20 p.-6 fig.Clinorotation was the first method designed to simulate microgravity on ground and it remains the most common and accessible simulation procedure. However, different experimental settings, namely angular velocity, sample orientation, and distance to the rotation center produce different responses in seedlings. Here, we compare A. thaliana root responses to the two most commonly used velocities, as examples of slow and fast clinorotation, and to vertical and horizontal clinorotation. We investigate their impact on the three stages of gravitropism: statolith sedimentation, asymmetrical auxin distribution, and differential elongation. We also investigate the statocyte ultrastructure by electron microscopy. Horizontal slow clinorotation induces changes in the statocyte ultrastructure related to a stress response and internalization of the PIN-FORMED 2 (PIN2) auxin transporter in the lower endodermis, probably due to enhanced mechano-stimulation. Additionally, fast clinorotation, as predicted, is only suitable within a very limited radius from the clinorotation center and triggers directional root growth according to the direction of the centrifugal force. Our study provides a full morphological picture of the stages of graviresponse in the root tip, and it is a valuable contribution to the field of microgravity simulation by clarifying the limitations of 2D-clinostats and proposing a proper use.This research was funded by Agencia Estatal de Investigación of the Spanish Ministry of Science and Innovation, Grants #ESP2015-64323-R and #RTI2018-099309-B-I00 (co-funded by EU-ERDF), awarded to FJM. The clinostat was obtained by a Grant of the United Nations Zero-Gravity Instrument Project (ZGIP), second and third cycle calls 2014/2018 (UNOOSA), awarded to RH and FJM. AV is a recipient of a contract of the Spanish National Program for Young Researchers Training (BES-2016-077976).Peer reviewedMultidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)United Nations Office for Outer Space AffairsVillacampa, Alicia [0000-0002-7398-8545]Sora, Ludovico [0000-0002-6520-6443]Herranz, Raúl [0000-0002-0246-9449]Medina, F. Javier [0000-0002-0866-7710]Ciska, Malgorzata [0000-0002-6514-9493]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/259722reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//ESP2015-64323-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099309-B-I00https://doi.org/10.3390/plants10040734Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2597222026-05-22T06:33:51Z
dc.title.none.fl_str_mv Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
title Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
spellingShingle Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
Villacampa, Alicia
Clinostat
Gravitropism
Gravity perception
Microgravity simulation
Plant
title_short Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
title_full Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
title_fullStr Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
title_full_unstemmed Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
title_sort Analysis of graviresponse and biological effects of vertical and horizontal clinorotation in Arabidopsis thaliana root tip
dc.creator.none.fl_str_mv Villacampa, Alicia
Sora, Ludovico
Herranz, Raúl
Medina, F. Javier
Ciska, Malgorzata
author Villacampa, Alicia
author_facet Villacampa, Alicia
Sora, Ludovico
Herranz, Raúl
Medina, F. Javier
Ciska, Malgorzata
author_role author
author2 Sora, Ludovico
Herranz, Raúl
Medina, F. Javier
Ciska, Malgorzata
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
United Nations Office for Outer Space Affairs
Villacampa, Alicia [0000-0002-7398-8545]
Sora, Ludovico [0000-0002-6520-6443]
Herranz, Raúl [0000-0002-0246-9449]
Medina, F. Javier [0000-0002-0866-7710]
Ciska, Malgorzata [0000-0002-6514-9493]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Clinostat
Gravitropism
Gravity perception
Microgravity simulation
Plant
topic Clinostat
Gravitropism
Gravity perception
Microgravity simulation
Plant
description 20 p.-6 fig.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/259722
url http://hdl.handle.net/10261/259722
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//ESP2015-64323-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099309-B-I00
https://doi.org/10.3390/plants10040734
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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