SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes

In fission yeast, MBF-dependent transcription is required for cells to complete S phase. The MBF transcription factor is regulated through a complex feedback mechanism that involves the co-repressors Yox1 and Nrm1 that are loaded onto MBF at the end of S phase, while positive transactivation is achi...

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Detalles Bibliográficos
Autores: González Medina, Alberto, 1990-, Pazo, Esther, Hidalgo Hernando, Elena, Ayté del Olmo, José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/52267
Acceso en línea:http://hdl.handle.net/10230/52267
http://dx.doi.org/10.1080/15384101.2021.2008203
Access Level:acceso abierto
Palabra clave:MBF
RSC complex
Start
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spelling SWI/SNF and RSC remodeler complexes bind to MBF-dependent genesGonzález Medina, Alberto, 1990-Pazo, EstherHidalgo Hernando, ElenaAyté del Olmo, JoséMBFRSC complexStartIn fission yeast, MBF-dependent transcription is required for cells to complete S phase. The MBF transcription factor is regulated through a complex feedback mechanism that involves the co-repressors Yox1 and Nrm1 that are loaded onto MBF at the end of S phase, while positive transactivation is achieved through the constitutive binding of the co-activator Rep2. Here we show that Rep2 is required to fully recruit the chromatin remodelers SWI/SNF and RSC to MBF-regulated promoters. On the contrary, Nrm1 and Yox1, when bound to the MBF complex, block the approximation of these chromatin remodelers to MBF-regulated promoters. We propose that SWI/SNF and RSC are recruited to MBF-regulated genes, and RSC together with SAGA complex are important to regulate the G1-to-S transcriptional wave. Mutants of these remodeler complexes are highly sensitive when cells are exposed to insults that challenge DNA synthesis.This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (MINECO) (Ministerio de Economía, Industria y Competitividad, Gobierno de España PGC2018-097248-B-I00), PLAN E and Feder and by Unidad de Excelencia María de Maeztu (CEX2018-000792-M). EH is recipient of an ICREA Academia Award (Instituci’ó Catalana de Recerca i Estudis Avançats, Generalitat de Catalunya).Taylor & Francis20222021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/52267http://dx.doi.org/10.1080/15384101.2021.2008203reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésCell Cycle. 2021;20(24):2652-61info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00© This is an Accepted Manuscript of an article published by Taylor & Francis in Cell cycle on 2021, available online: http://www.tandfonline.com/10.1080/15384101.2021.2008203.info:eu-repo/semantics/openAccessoai:recercat.cat:10230/522672026-05-29T05:05:01Z
dc.title.none.fl_str_mv SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
title SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
spellingShingle SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
González Medina, Alberto, 1990-
MBF
RSC complex
Start
title_short SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
title_full SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
title_fullStr SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
title_full_unstemmed SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
title_sort SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes
dc.creator.none.fl_str_mv González Medina, Alberto, 1990-
Pazo, Esther
Hidalgo Hernando, Elena
Ayté del Olmo, José
author González Medina, Alberto, 1990-
author_facet González Medina, Alberto, 1990-
Pazo, Esther
Hidalgo Hernando, Elena
Ayté del Olmo, José
author_role author
author2 Pazo, Esther
Hidalgo Hernando, Elena
Ayté del Olmo, José
author2_role author
author
author
dc.subject.none.fl_str_mv MBF
RSC complex
Start
topic MBF
RSC complex
Start
description In fission yeast, MBF-dependent transcription is required for cells to complete S phase. The MBF transcription factor is regulated through a complex feedback mechanism that involves the co-repressors Yox1 and Nrm1 that are loaded onto MBF at the end of S phase, while positive transactivation is achieved through the constitutive binding of the co-activator Rep2. Here we show that Rep2 is required to fully recruit the chromatin remodelers SWI/SNF and RSC to MBF-regulated promoters. On the contrary, Nrm1 and Yox1, when bound to the MBF complex, block the approximation of these chromatin remodelers to MBF-regulated promoters. We propose that SWI/SNF and RSC are recruited to MBF-regulated genes, and RSC together with SAGA complex are important to regulate the G1-to-S transcriptional wave. Mutants of these remodeler complexes are highly sensitive when cells are exposed to insults that challenge DNA synthesis.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/52267
http://dx.doi.org/10.1080/15384101.2021.2008203
url http://hdl.handle.net/10230/52267
http://dx.doi.org/10.1080/15384101.2021.2008203
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cell Cycle. 2021;20(24):2652-61
info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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