Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

[EN] Self-assembling peptides (SAP) are widely used as scaffolds themselves, and recently as fillers of microporous scaffolds, where the former provides a cell-friendly nanoenvironment and the latter improves its mechanical properties. The characterization of the interaction between these short pept...

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Detalhes bibliográficos
Autores: Arnal Pastor, María Pilar, González-Mora, Debora, García-Torres, Fernando, Monleón Pradas, Manuel|||0000-0001-6457-0414, Vallés Lluch, Ana|||0000-0002-7896-8666
Tipo de documento: artigo
Data de publicação:2016
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositório:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglês
OAI Identifier:oai:riunet.upv.es:10251/81033
Acesso em linha:https://riunet.upv.es/handle/10251/81033
Access Level:Acceso aberto
Palavra-chave:Self-assembling peptide
Ethyl acrylate
Acrylic acid
Substrate interaction
Wettability
Electron Microscopy Service of the UPV
MAQUINAS Y MOTORES TERMICOS
TERMODINAMICA APLICADA (UPV)
Descrição
Resumo:[EN] Self-assembling peptides (SAP) are widely used as scaffolds themselves, and recently as fillers of microporous scaffolds, where the former provides a cell-friendly nanoenvironment and the latter improves its mechanical properties. The characterization of the interaction between these short peptides and the scaffold material is crucial to assess the potential of such a combined system. In this work, the interaction between poly(ethyl acrylate) (PEA) and 90/10 ethyl acrylate-acrylic acid copolymer P(EAcoAAc) with the SAP RAD16-I has been followed using a bidimensional simplified model. By means of the techniques of choice (congo red staining, atomic force microscopy (AFM), and contact angle measurements) the interaction and self-assembly of the peptide has proven to be very sensitive to the wettability and electro-negativity of the polymeric substrate.