Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers

During the last few years, membrane vesicles (as exovesicles) have emerged as potential nanocarriers for therapeutic applications. They are receiving attention due to their proteo-lipid nature, size, biocompatibility and biodegradability. In this work, we investigated the potential use of isolated r...

Full description

Bibliographic Details
Authors: Yepes-Molina, Lucía, Martínez-Ballesta, M. Carmen, Carvajal, Micaela
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/237312
Online Access:http://hdl.handle.net/10261/237312
Access Level:Open access
Keyword:Broccoli
Encapsulation
Keratinocytes
Nanocarriers
Skin
id ES_7f3ae6b6dcdaf49253d3e8bc112c52bd
oai_identifier_str oai:digital.csic.es:10261/237312
network_acronym_str ES
network_name_str España
repository_id_str
spelling Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriersYepes-Molina, LucíaMartínez-Ballesta, M. CarmenCarvajal, MicaelaBroccoliEncapsulationKeratinocytesNanocarriersSkinDuring the last few years, membrane vesicles (as exovesicles) have emerged as potential nanocarriers for therapeutic applications. They are receiving attention due to their proteo-lipid nature, size, biocompatibility and biodegradability. In this work, we investigated the potential use of isolated root plasma membrane vesicles from broccoli plants as nanocarriers. For that, the entrapment efficiency and integrity of the vesicles were determined. Also, the delivery of keratinocytes and penetrability through skin were studied. The results show that the broccoli vesicles had high stability, in relation to their proteins, and high entrapment efficiency. Also, the interaction between the vesicles and keratinocytes was proven by the delivery of an encapsulated fluorescent product into cells and by the detection of plant proteins in the keratinocyte plasma membrane, showing the interactions between the membranes of two species of distinct biological kingdoms. Therefore, these results, together with the capacity of brassica vesicles to cross the skin layers, detected by fluorescent penetration, enable us to propose a type of nanocarrier obtained from natural plant membranes for use in transdermal delivery.This work was funded by the Spanish Ministerio de Economía, Industria y Competitividad (AGL2016-80247-C2-1-R).ElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/237312reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80247-C2-1-Rhttp://dx.doi.org/10.1016/j.jare.2020.02.004Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2373122026-05-22T06:33:51Z
dc.title.none.fl_str_mv Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
title Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
spellingShingle Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
Yepes-Molina, Lucía
Broccoli
Encapsulation
Keratinocytes
Nanocarriers
Skin
title_short Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
title_full Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
title_fullStr Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
title_full_unstemmed Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
title_sort Plant plasma membrane vesicles interaction with keratinocytes reveals their potential as carriers
dc.creator.none.fl_str_mv Yepes-Molina, Lucía
Martínez-Ballesta, M. Carmen
Carvajal, Micaela
author Yepes-Molina, Lucía
author_facet Yepes-Molina, Lucía
Martínez-Ballesta, M. Carmen
Carvajal, Micaela
author_role author
author2 Martínez-Ballesta, M. Carmen
Carvajal, Micaela
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Broccoli
Encapsulation
Keratinocytes
Nanocarriers
Skin
topic Broccoli
Encapsulation
Keratinocytes
Nanocarriers
Skin
description During the last few years, membrane vesicles (as exovesicles) have emerged as potential nanocarriers for therapeutic applications. They are receiving attention due to their proteo-lipid nature, size, biocompatibility and biodegradability. In this work, we investigated the potential use of isolated root plasma membrane vesicles from broccoli plants as nanocarriers. For that, the entrapment efficiency and integrity of the vesicles were determined. Also, the delivery of keratinocytes and penetrability through skin were studied. The results show that the broccoli vesicles had high stability, in relation to their proteins, and high entrapment efficiency. Also, the interaction between the vesicles and keratinocytes was proven by the delivery of an encapsulated fluorescent product into cells and by the detection of plant proteins in the keratinocyte plasma membrane, showing the interactions between the membranes of two species of distinct biological kingdoms. Therefore, these results, together with the capacity of brassica vesicles to cross the skin layers, detected by fluorescent penetration, enable us to propose a type of nanocarrier obtained from natural plant membranes for use in transdermal delivery.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/237312
url http://hdl.handle.net/10261/237312
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80247-C2-1-R
http://dx.doi.org/10.1016/j.jare.2020.02.004
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411808502087680
score 15,812429

Similar Items