Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications

Oligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides carrying peptide sequences at the 3¢-end on a single support. The resulting oligonucleotide–...

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Detalhes bibliográficos
Autores: Aviñó, Anna, Grijalvo, Santiago, Pérez-Rentero, Sonia, Garibotti, Alejandra V., Terrazas, Montserrat, Eritja Casadellà, Ramón
Formato: otro
Fecha de publicación:2001
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/54172
Acesso em linha:http://hdl.handle.net/10261/54172
Access Level:acceso abierto
Palavra-chave:DNA
RNA
Nucleic acids
RNA interference
Antisense
Oligonucleotide–peptide conjugates
Stepwise synthesis
Postsynthetic conjugation
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spelling Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological ApplicationsAviñó, AnnaGrijalvo, SantiagoPérez-Rentero, SoniaGaribotti, Alejandra V.Terrazas, MontserratEritja Casadellà, RamónDNARNANucleic acidsRNA interferenceAntisenseOligonucleotide–peptide conjugatesStepwise synthesisPostsynthetic conjugationOligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides carrying peptide sequences at the 3¢-end on a single support. The resulting oligonucleotide–peptide conjugates may be used as exogenous effectors for the specific control of gene expression. In the second part of this chapter, detailed postsynthetic conjugation protocols to introduce peptide sequences into oligonucleotide sequences are also presented.This work is supported by the Spanish Ministry of Education (BFU2007-63287/BMC), Generalitat de Catalunya (2009/ SGR/238), Instituto de Salud Carlos III (CIBER-BNN), and European Communities (DYNAMO contract 028669 (NEST), FUNMOL NMP4-SL-2009-213382).Peer reviewedHumana PressMinisterio de Educación y Ciencia (España)Generalitat de CatalunyaInstituto de Salud Carlos IIIEuropean Commission201220122001info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248info:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/54172reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/213382Methods in Molecular Biology751http://www.dx.doi.org/10.1007/978-1-61779-151-2_14info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/541722026-05-22T06:33:51Z
dc.title.none.fl_str_mv Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
title Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
spellingShingle Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
Aviñó, Anna
DNA
RNA
Nucleic acids
RNA interference
Antisense
Oligonucleotide–peptide conjugates
Stepwise synthesis
Postsynthetic conjugation
title_short Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
title_full Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
title_fullStr Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
title_full_unstemmed Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
title_sort Synthesis of Oligonucleotide–Peptide Conjugates for Biomedical and Technological Applications
dc.creator.none.fl_str_mv Aviñó, Anna
Grijalvo, Santiago
Pérez-Rentero, Sonia
Garibotti, Alejandra V.
Terrazas, Montserrat
Eritja Casadellà, Ramón
author Aviñó, Anna
author_facet Aviñó, Anna
Grijalvo, Santiago
Pérez-Rentero, Sonia
Garibotti, Alejandra V.
Terrazas, Montserrat
Eritja Casadellà, Ramón
author_role author
author2 Grijalvo, Santiago
Pérez-Rentero, Sonia
Garibotti, Alejandra V.
Terrazas, Montserrat
Eritja Casadellà, Ramón
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
Generalitat de Catalunya
Instituto de Salud Carlos III
European Commission
dc.subject.none.fl_str_mv DNA
RNA
Nucleic acids
RNA interference
Antisense
Oligonucleotide–peptide conjugates
Stepwise synthesis
Postsynthetic conjugation
topic DNA
RNA
Nucleic acids
RNA interference
Antisense
Oligonucleotide–peptide conjugates
Stepwise synthesis
Postsynthetic conjugation
description Oligonucleotide–peptide conjugates have attracted considerable interest especially for biomedical uses. In the first part of this chapter, we describe protocols for the stepwise synthesis of oligonucleotides carrying peptide sequences at the 3¢-end on a single support. The resulting oligonucleotide–peptide conjugates may be used as exogenous effectors for the specific control of gene expression. In the second part of this chapter, detailed postsynthetic conjugation protocols to introduce peptide sequences into oligonucleotide sequences are also presented.
publishDate 2001
dc.date.none.fl_str_mv 2001
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/54172
url http://hdl.handle.net/10261/54172
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/213382
Methods in Molecular Biology
751
http://www.dx.doi.org/10.1007/978-1-61779-151-2_14
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411804655910912
score 15.81155

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