Presence of Ceramidase Activity in Electronegative LDL
Electronegative low-density lipoprotein (LDL(-)) is a minor modified fraction of human plasma LDL with several atherogenic properties. Among them is increased bioactive lipid mediator content, such as lysophosphatidylcholine (LPC), non-esterified fatty acids (NEFA), ceramide (Cer), and sphingosine (...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:280926 |
| Acceso en línea: | https://ddd.uab.cat/record/280926 https://dx.doi.org/urn:doi:10.3390/ijms24010165 |
| Access Level: | acceso abierto |
| Palabra clave: | Ceramidase Ceramide Electronegative LDL Sphingomyelinase Sphingosine |
| id |
ES_7f2c3cd3377ef2681bc8eecf8c987c3c |
|---|---|
| oai_identifier_str |
oai:ddd.uab.cat:280926 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Presence of Ceramidase Activity in Electronegative LDLPuig Calvó, Núria|||0000-0001-8235-4934Rives Jimenez, Jose|||0009-0001-5725-9270Estruch, Montserrat|||0000-0002-7162-4158Aguilera-Simón, Ana|||0000-0003-1428-8212Rotllan, Noemi|||0000-0002-0587-8045Camacho, Mercedes|||0000-0001-5970-3294Colomé, NúriaCanals, Francesc|||0000-0002-0650-1135Sánchez Quesada, José Luis|||0000-0003-0224-591XBenitez, Sonia|||0000-0003-3565-0743CeramidaseCeramideElectronegative LDLSphingomyelinaseSphingosineElectronegative low-density lipoprotein (LDL(-)) is a minor modified fraction of human plasma LDL with several atherogenic properties. Among them is increased bioactive lipid mediator content, such as lysophosphatidylcholine (LPC), non-esterified fatty acids (NEFA), ceramide (Cer), and sphingosine (Sph), which are related to the presence of some phospholipolytic activities, including platelet-activating factor acetylhydrolase (PAF-AH), phospholipase C (PLC), and sphingomyelinase (SMase), in LDL(-). However, these enzymes' activities do not explain the increased Sph content, which typically derives from Cer degradation. In the present study, we analyzed the putative presence of ceramidase (CDase) activity, which could explain the increased Sph content. Thin layer chromatography (TLC) and lipidomic analysis showed that Cer, Sph, and NEFA spontaneously increased in LDL(-) incubated alone at 37 °C, in contrast with native LDL(+). An inhibitor of neutral CDase prevented the formation of Sph and, in turn, increased Cer content in LDL(-). In addition, LDL(-) efficiently degraded fluorescently labeled Cer (NBD-Cer) to form Sph and NEFA. These observations defend the existence of the CDase-like activity's association with LDL(-). However, neither the proteomic analysis nor the Western blot detected the presence of an enzyme with known CDase activity. Further studies are thus warranted to define the origin of the CDase-like activity detected in LDL(-).Universitat Autònoma de Barcelona 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/280926https://dx.doi.org/urn:doi:10.3390/ijms24010165reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI13/00364Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI16/00471Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI20/00334Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00257Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0019/0010open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2809262026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Presence of Ceramidase Activity in Electronegative LDL |
| title |
Presence of Ceramidase Activity in Electronegative LDL |
| spellingShingle |
Presence of Ceramidase Activity in Electronegative LDL Puig Calvó, Núria|||0000-0001-8235-4934 Ceramidase Ceramide Electronegative LDL Sphingomyelinase Sphingosine |
| title_short |
Presence of Ceramidase Activity in Electronegative LDL |
| title_full |
Presence of Ceramidase Activity in Electronegative LDL |
| title_fullStr |
Presence of Ceramidase Activity in Electronegative LDL |
| title_full_unstemmed |
Presence of Ceramidase Activity in Electronegative LDL |
| title_sort |
Presence of Ceramidase Activity in Electronegative LDL |
| dc.creator.none.fl_str_mv |
Puig Calvó, Núria|||0000-0001-8235-4934 Rives Jimenez, Jose|||0009-0001-5725-9270 Estruch, Montserrat|||0000-0002-7162-4158 Aguilera-Simón, Ana|||0000-0003-1428-8212 Rotllan, Noemi|||0000-0002-0587-8045 Camacho, Mercedes|||0000-0001-5970-3294 Colomé, Núria Canals, Francesc|||0000-0002-0650-1135 Sánchez Quesada, José Luis|||0000-0003-0224-591X Benitez, Sonia|||0000-0003-3565-0743 |
| author |
Puig Calvó, Núria|||0000-0001-8235-4934 |
| author_facet |
Puig Calvó, Núria|||0000-0001-8235-4934 Rives Jimenez, Jose|||0009-0001-5725-9270 Estruch, Montserrat|||0000-0002-7162-4158 Aguilera-Simón, Ana|||0000-0003-1428-8212 Rotllan, Noemi|||0000-0002-0587-8045 Camacho, Mercedes|||0000-0001-5970-3294 Colomé, Núria Canals, Francesc|||0000-0002-0650-1135 Sánchez Quesada, José Luis|||0000-0003-0224-591X Benitez, Sonia|||0000-0003-3565-0743 |
| author_role |
author |
| author2 |
Rives Jimenez, Jose|||0009-0001-5725-9270 Estruch, Montserrat|||0000-0002-7162-4158 Aguilera-Simón, Ana|||0000-0003-1428-8212 Rotllan, Noemi|||0000-0002-0587-8045 Camacho, Mercedes|||0000-0001-5970-3294 Colomé, Núria Canals, Francesc|||0000-0002-0650-1135 Sánchez Quesada, José Luis|||0000-0003-0224-591X Benitez, Sonia|||0000-0003-3565-0743 |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Ceramidase Ceramide Electronegative LDL Sphingomyelinase Sphingosine |
| topic |
Ceramidase Ceramide Electronegative LDL Sphingomyelinase Sphingosine |
| description |
Electronegative low-density lipoprotein (LDL(-)) is a minor modified fraction of human plasma LDL with several atherogenic properties. Among them is increased bioactive lipid mediator content, such as lysophosphatidylcholine (LPC), non-esterified fatty acids (NEFA), ceramide (Cer), and sphingosine (Sph), which are related to the presence of some phospholipolytic activities, including platelet-activating factor acetylhydrolase (PAF-AH), phospholipase C (PLC), and sphingomyelinase (SMase), in LDL(-). However, these enzymes' activities do not explain the increased Sph content, which typically derives from Cer degradation. In the present study, we analyzed the putative presence of ceramidase (CDase) activity, which could explain the increased Sph content. Thin layer chromatography (TLC) and lipidomic analysis showed that Cer, Sph, and NEFA spontaneously increased in LDL(-) incubated alone at 37 °C, in contrast with native LDL(+). An inhibitor of neutral CDase prevented the formation of Sph and, in turn, increased Cer content in LDL(-). In addition, LDL(-) efficiently degraded fluorescently labeled Cer (NBD-Cer) to form Sph and NEFA. These observations defend the existence of the CDase-like activity's association with LDL(-). However, neither the proteomic analysis nor the Western blot detected the presence of an enzyme with known CDase activity. Further studies are thus warranted to define the origin of the CDase-like activity detected in LDL(-). |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2 2022-01-01 2022 2022-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/280926 https://dx.doi.org/urn:doi:10.3390/ijms24010165 |
| url |
https://ddd.uab.cat/record/280926 https://dx.doi.org/urn:doi:10.3390/ijms24010165 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI13/00364 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI16/00471 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI20/00334 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CB16/11/00257 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0019/0010 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
| instname_str |
Universitat Autònoma de Barcelona |
| reponame_str |
Dipòsit Digital de Documents de la UAB |
| collection |
Dipòsit Digital de Documents de la UAB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869411804620259328 |
| score |
15.300719 |