Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes

A sea fennel (Crithmum maritimum) aqueous extract was prepared and loaded into soybean phosphatidylcholine liposomes. Both the free extract (FE), and the empty (L) and loaded (L-FE) liposomes were shown to be non-cytotoxic to THP-1 and Caco-2 cells. The anti-inflammatory effect was tested on THP-1 c...

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Autores: Alemán, Ailén, Marín, Daniel, Fernández de Palencia, P., Gómez Guillén, M. C., Montero García, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/285352
Acceso en línea:http://hdl.handle.net/10261/285352
Access Level:acceso abierto
Palabra clave:Sea fennel
Intestinal absorption
Phosphatidylcholine liposomes
Gastrointestinal digestion
Anti-inflammatory properties
Chlorogenic acid
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spelling Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomesAlemán, AilénMarín, DanielFernández de Palencia, P.Gómez Guillén, M. C.Montero García, PilarSea fennelIntestinal absorptionPhosphatidylcholine liposomesGastrointestinal digestionAnti-inflammatory propertiesChlorogenic acidA sea fennel (Crithmum maritimum) aqueous extract was prepared and loaded into soybean phosphatidylcholine liposomes. Both the free extract (FE), and the empty (L) and loaded (L-FE) liposomes were shown to be non-cytotoxic to THP-1 and Caco-2 cells. The anti-inflammatory effect was tested on THP-1 cells differentiated into macrophages. FE showed anti-inflammatory activity, revealed by the induced secretion of IL-10 cytokines in macrophages that were subsequently stimulated with LPS. Also, a decrease in TNF-α production by L was observed, evidencing that liposomes reduced the pro-inflammatory mediators’ secretion. The liposomes (L) showed protective anti-inflammatory activity and also were able to downregulate the inflammation. Furthermore, L-FE were also found to downregulate the inflammation response, as they were able to decrease TNF-α secretion in macrophages previously exposed to LPS. The simulated in vitro gastrointestinal digestion (GID) of FE diminished the chlorogenic acid content (the main polyphenolic compound of the extract) by 40%, while in L-FE, the amount of this phenolic compound increased with respect to the undigested liposomes. The amount of bioaccessible chlorogenic, however, was similar for FE and L-FE. The percentage of chlorogenic acid absorbed through a Caco-2 cell monolayer after 3 h of incubation, was significantly similar for the extract and the liposomes (~1.5%), without finding significant differences once the extract and liposomes were digested.This work was supported by the Spanish Ministry of Economy and Competitiveness project NANOALIVAL AGL2017-84161, cofounded with ERDF (European Regional Development Fund) and CSIC -202070E218.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)European CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/285352reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84161-C2-1-RThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/nu14010210https://doi.org/10.3390/nu14010210Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2853522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
title Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
spellingShingle Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
Alemán, Ailén
Sea fennel
Intestinal absorption
Phosphatidylcholine liposomes
Gastrointestinal digestion
Anti-inflammatory properties
Chlorogenic acid
title_short Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
title_full Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
title_fullStr Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
title_full_unstemmed Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
title_sort Anti-inflammatory properties, bioaccessibility and Intestinal absorption of sea fennel (Crithmum maritimum) extract encapsulated in soy phosphatidylcholine liposomes
dc.creator.none.fl_str_mv Alemán, Ailén
Marín, Daniel
Fernández de Palencia, P.
Gómez Guillén, M. C.
Montero García, Pilar
author Alemán, Ailén
author_facet Alemán, Ailén
Marín, Daniel
Fernández de Palencia, P.
Gómez Guillén, M. C.
Montero García, Pilar
author_role author
author2 Marín, Daniel
Fernández de Palencia, P.
Gómez Guillén, M. C.
Montero García, Pilar
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sea fennel
Intestinal absorption
Phosphatidylcholine liposomes
Gastrointestinal digestion
Anti-inflammatory properties
Chlorogenic acid
topic Sea fennel
Intestinal absorption
Phosphatidylcholine liposomes
Gastrointestinal digestion
Anti-inflammatory properties
Chlorogenic acid
description A sea fennel (Crithmum maritimum) aqueous extract was prepared and loaded into soybean phosphatidylcholine liposomes. Both the free extract (FE), and the empty (L) and loaded (L-FE) liposomes were shown to be non-cytotoxic to THP-1 and Caco-2 cells. The anti-inflammatory effect was tested on THP-1 cells differentiated into macrophages. FE showed anti-inflammatory activity, revealed by the induced secretion of IL-10 cytokines in macrophages that were subsequently stimulated with LPS. Also, a decrease in TNF-α production by L was observed, evidencing that liposomes reduced the pro-inflammatory mediators’ secretion. The liposomes (L) showed protective anti-inflammatory activity and also were able to downregulate the inflammation. Furthermore, L-FE were also found to downregulate the inflammation response, as they were able to decrease TNF-α secretion in macrophages previously exposed to LPS. The simulated in vitro gastrointestinal digestion (GID) of FE diminished the chlorogenic acid content (the main polyphenolic compound of the extract) by 40%, while in L-FE, the amount of this phenolic compound increased with respect to the undigested liposomes. The amount of bioaccessible chlorogenic, however, was similar for FE and L-FE. The percentage of chlorogenic acid absorbed through a Caco-2 cell monolayer after 3 h of incubation, was significantly similar for the extract and the liposomes (~1.5%), without finding significant differences once the extract and liposomes were digested.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/285352
url http://hdl.handle.net/10261/285352
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84161-C2-1-R
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/nu14010210
https://doi.org/10.3390/nu14010210
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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