Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS

In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT...

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Detalles Bibliográficos
Autores: Valdés, Alberto, Bergquist, Jonas, García-Cañas, Virginia, Cifuentes, Alejandro
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/150109
Acceso en línea:http://hdl.handle.net/10261/150109
Access Level:acceso abierto
Palabra clave:Rosemary extract
Antiproliferative activity
Quantitative proteomics
Mass spectrometry
HT-29 cells
Colon cancer
Dimethyl labeling
Foodomics
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spelling Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MSValdés, AlbertoBergquist, JonasGarcía-Cañas, VirginiaCifuentes, AlejandroRosemary extractAntiproliferative activityQuantitative proteomicsMass spectrometryHT-29 cellsColon cancerDimethyl labelingFoodomicsIn this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress.This work was supported by the projects AGL2014-53609-P (Ministerio de Economia y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economia y Competitividad for his FPI predoctoral fellowship (BES-2012-057014). The Swedish Research Council (2011-4423 and 2015-4870; J.B.) is acknowledged for financial support.Peer reviewedPeer ReviewedAmerican Chemical SocietySwedish Research CouncilMinisterio de Economía y Competitividad (España)Comunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/150109reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-PS2013/ABI-2728/ALIBIRD-CMThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1021/acs.jproteome.6b00154https://doi.org/10.1021/acs.jproteome.6b00154Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1501092026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
title Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
spellingShingle Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
Valdés, Alberto
Rosemary extract
Antiproliferative activity
Quantitative proteomics
Mass spectrometry
HT-29 cells
Colon cancer
Dimethyl labeling
Foodomics
title_short Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
title_full Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
title_fullStr Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
title_full_unstemmed Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
title_sort Comprehensive proteomic study of the antiproliferative activity of a polyphenol-enriched rosemary extract on colon cancer cells using nanoliquid chromatography-orbitrap MS/MS
dc.creator.none.fl_str_mv Valdés, Alberto
Bergquist, Jonas
García-Cañas, Virginia
Cifuentes, Alejandro
author Valdés, Alberto
author_facet Valdés, Alberto
Bergquist, Jonas
García-Cañas, Virginia
Cifuentes, Alejandro
author_role author
author2 Bergquist, Jonas
García-Cañas, Virginia
Cifuentes, Alejandro
author2_role author
author
author
dc.contributor.none.fl_str_mv Swedish Research Council
Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Rosemary extract
Antiproliferative activity
Quantitative proteomics
Mass spectrometry
HT-29 cells
Colon cancer
Dimethyl labeling
Foodomics
topic Rosemary extract
Antiproliferative activity
Quantitative proteomics
Mass spectrometry
HT-29 cells
Colon cancer
Dimethyl labeling
Foodomics
description In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/150109
url http://hdl.handle.net/10261/150109
dc.language.none.fl_str_mv Inglés
Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-P
S2013/ABI-2728/ALIBIRD-CM
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1021/acs.jproteome.6b00154
https://doi.org/10.1021/acs.jproteome.6b00154
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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