Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain

BACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) ass...

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Autores: Adell-Aledón, Manuel, Köster, Pamela Carolina, de Lucio, Aida, Puente, Paula, Hernandez-De-Mingo, Marta, Sánchez-Thevenet, Paula, Dea-Ayuela, María Auxiliadora, Carmena, David
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/7055
Acceso en línea:http://hdl.handle.net/20.500.12105/7055
Access Level:acceso abierto
Palabra clave:Animals
Cross-Sectional Studies
Dog Diseases
Dogs
Feces
Female
Genes, rRNA
Giardia lamblia
Giardiasis
Male
Microscopy, Fluorescence
Molecular Epidemiology
Spain
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repository_id_str
spelling Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern SpainAdell-Aledón, ManuelKöster, Pamela Carolinade Lucio, AidaPuente, PaulaHernandez-De-Mingo, MartaSánchez-Thevenet, PaulaDea-Ayuela, María AuxiliadoraCarmena, DavidAnimalsCross-Sectional StudiesDog DiseasesDogsFecesFemaleGenes, rRNAGiardia lambliaGiardiasisMaleMicroscopy, FluorescenceMolecular EpidemiologySpainBACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) assemblages of G. duodenalis. However, zoonotic assemblages A and B have been increasingly documented in canine isolates, raising the question of whether and to which extent dogs can act as natural reservoirs of human giardiosis. METHODS: In this cross-sectional epidemiological survey we assessed the molecular diversity of G. duodenalis in dogs in the province of Castellón, Eastern Spain. A total of 348 individual faecal samples from sheltered (n = 218), breeding (n = 24), hunting (n = 68), shepherd (n = 24), and pet (n = 14) dogs were collected between 2014 and 2016. Detection of G. duodenalis cysts in faecal material was carried out by direct fluorescence microscopy as a screening test, whereas a qPCR targeting the small subunit ribosomal RNA gene of the parasite was subsequently used as a confirmatory method. RESULTS: Giardia duodenalis was detected in 36.5% (95% CI: 31.6-41.7%) of dogs. No significant differences in prevalence rates could be demonstrated among dogs according to their sex and geographical origin, but breeding (45.8%; 95% CI: 27.9-64.9%) and sheltered (40.4%; 95% CI: 34.1-47.0%) dogs harboured significantly higher proportions of G. duodenalis. Multi-locus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of G. duodenalis allowed the characterization of 35 canine isolates that were unambiguously assigned to assemblages A (14.3%), B (22.9%), C (5.7%), and D (37.1%). A number of inter-assemblage mixed infections including A + B (11.4%), A + D (2.9%), and A + B + D (5.7%) were also identified. CONCLUSIONS: Data presented here are strongly indicative of high infection pressures in kennelled animals. Zoonotic sub-assemblages AII, BIII, and BIV were responsible for a considerable proportion of the G. duodenalis infections detected, but very few of the genotypes identified have been previously documented in Spanish human populations. Although possible, zoonotic transmission between dogs and humans seems an infrequent event in this Spanish region.BioMed Central (BMC)Instituto de Salud Carlos III20192019-02-0120182018-01-2220182018-01-22journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/7055reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES CP12 03081open accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/70552026-06-12T12:43:37Z
dc.title.none.fl_str_mv Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
title Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
spellingShingle Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
Adell-Aledón, Manuel
Animals
Cross-Sectional Studies
Dog Diseases
Dogs
Feces
Female
Genes, rRNA
Giardia lamblia
Giardiasis
Male
Microscopy, Fluorescence
Molecular Epidemiology
Spain
title_short Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
title_full Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
title_fullStr Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
title_full_unstemmed Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
title_sort Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
dc.creator.none.fl_str_mv Adell-Aledón, Manuel
Köster, Pamela Carolina
de Lucio, Aida
Puente, Paula
Hernandez-De-Mingo, Marta
Sánchez-Thevenet, Paula
Dea-Ayuela, María Auxiliadora
Carmena, David
author Adell-Aledón, Manuel
author_facet Adell-Aledón, Manuel
Köster, Pamela Carolina
de Lucio, Aida
Puente, Paula
Hernandez-De-Mingo, Marta
Sánchez-Thevenet, Paula
Dea-Ayuela, María Auxiliadora
Carmena, David
author_role author
author2 Köster, Pamela Carolina
de Lucio, Aida
Puente, Paula
Hernandez-De-Mingo, Marta
Sánchez-Thevenet, Paula
Dea-Ayuela, María Auxiliadora
Carmena, David
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Animals
Cross-Sectional Studies
Dog Diseases
Dogs
Feces
Female
Genes, rRNA
Giardia lamblia
Giardiasis
Male
Microscopy, Fluorescence
Molecular Epidemiology
Spain
topic Animals
Cross-Sectional Studies
Dog Diseases
Dogs
Feces
Female
Genes, rRNA
Giardia lamblia
Giardiasis
Male
Microscopy, Fluorescence
Molecular Epidemiology
Spain
description BACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) assemblages of G. duodenalis. However, zoonotic assemblages A and B have been increasingly documented in canine isolates, raising the question of whether and to which extent dogs can act as natural reservoirs of human giardiosis. METHODS: In this cross-sectional epidemiological survey we assessed the molecular diversity of G. duodenalis in dogs in the province of Castellón, Eastern Spain. A total of 348 individual faecal samples from sheltered (n = 218), breeding (n = 24), hunting (n = 68), shepherd (n = 24), and pet (n = 14) dogs were collected between 2014 and 2016. Detection of G. duodenalis cysts in faecal material was carried out by direct fluorescence microscopy as a screening test, whereas a qPCR targeting the small subunit ribosomal RNA gene of the parasite was subsequently used as a confirmatory method. RESULTS: Giardia duodenalis was detected in 36.5% (95% CI: 31.6-41.7%) of dogs. No significant differences in prevalence rates could be demonstrated among dogs according to their sex and geographical origin, but breeding (45.8%; 95% CI: 27.9-64.9%) and sheltered (40.4%; 95% CI: 34.1-47.0%) dogs harboured significantly higher proportions of G. duodenalis. Multi-locus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of G. duodenalis allowed the characterization of 35 canine isolates that were unambiguously assigned to assemblages A (14.3%), B (22.9%), C (5.7%), and D (37.1%). A number of inter-assemblage mixed infections including A + B (11.4%), A + D (2.9%), and A + B + D (5.7%) were also identified. CONCLUSIONS: Data presented here are strongly indicative of high infection pressures in kennelled animals. Zoonotic sub-assemblages AII, BIII, and BIV were responsible for a considerable proportion of the G. duodenalis infections detected, but very few of the genotypes identified have been previously documented in Spanish human populations. Although possible, zoonotic transmission between dogs and humans seems an infrequent event in this Spanish region.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-22
2018
2018-01-22
2019
2019-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/7055
url http://hdl.handle.net/20.500.12105/7055
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES CP12 03081
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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