Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242

Purpose Obesity during childhood has become a pandemic disease, mainly caused by a diet rich in sugars and fatty acids. Among other negative effects, these diets can induce cognitive impairment and reduce neuroplasticity. It is well known that omega-3 and probiotics have a beneficial impact on healt...

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Autores: Huguet, Gemma, Puig‑Parnau, Irene, Serrano Casasola, José Carlos Enrique, Martín Garí, Meritxell, Rodríguez Palmero, María, Moreno‑Muñoz, José Antonio, Tibau, Joan, Kádár, Elisabet
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/464194
Acceso en línea:https://doi.org/10.1007/s00394-023-03165-1
https://hdl.handle.net/10459.1/464194
Access Level:acceso abierto
Palabra clave:Pig
Neurogenesis
Neural plasticity
Doublecortin
Arc
Hippocampus
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spelling Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242Huguet, GemmaPuig‑Parnau, IreneSerrano Casasola, José Carlos EnriqueMartín Garí, MeritxellRodríguez Palmero, MaríaMoreno‑Muñoz, José AntonioTibau, JoanKádár, ElisabetPigNeurogenesisNeural plasticityDoublecortinArcHippocampusPurpose Obesity during childhood has become a pandemic disease, mainly caused by a diet rich in sugars and fatty acids. Among other negative effects, these diets can induce cognitive impairment and reduce neuroplasticity. It is well known that omega-3 and probiotics have a beneficial impact on health and cognition, and we have hypothesized that a diet enriched with Bifidobacterium breve and omega-3 could potentiate neuroplasticity in prepubertal pigs on a high-fat diet. Methods Young female piglets were fed during 10 weeks with: standard diet (T1), high-fat (HF) diet (T2), HF diet including B. breve CECT8242 (T3) and HF diet including the probiotic and omega-3 fatty acids (T4). Using hippocampal sections, we analyzed by immunocytochemistry the levels of doublecortin (DCX) to study neurogenesis, and activity-regulated cytoskeleton-associated protein (Arc) as a synaptic plasticity related protein. Results No effect of T2 or T3 was observed, whereas T4 increased both DCX+ cells and Arc expression. Therefore, a diet enriched with supplements of B. breve and omega-3 increases neurogenesis and synaptic plasticity in prepubertal females on a HF diet from nine weeks of age to sexual maturity. Furthermore, the analysis of serum cholesterol and HDL indicate that neurogenesis was related to lipidic demand in piglets fed with control or HF diets, but the neurogenic effect induced by the T4 diet was exerted by mechanisms independent of this lipidic demand. Conclusion Our results show that the T4 dietary treatment is effective in potentiating neural plasticity in the dorsal hippocampus of prepubertal females on a HF diet.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This project has been supported by Laboratorios Ordesa S.L., CDTI (Centro para el desarrollo Tecnológico e Industrial, Spain) and Fondo Europeo de Desarrollo Regional (FEDER; Acronym: INCOMES, project number IPT-20111008). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.Springer Nature2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1007/s00394-023-03165-1https://hdl.handle.net/10459.1/464194reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a https://doi.org/10.1007/s00394-023-03165-1European Journal of Nutrition, 2023, vol. 62, p. 2463–2473cc-by (c) Gemma Huguet et al., 2023Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4641942026-06-24T12:42:17Z
dc.title.none.fl_str_mv Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
title Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
spellingShingle Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
Huguet, Gemma
Pig
Neurogenesis
Neural plasticity
Doublecortin
Arc
Hippocampus
title_short Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
title_full Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
title_fullStr Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
title_full_unstemmed Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
title_sort Hippocampal neurogenesis and Arc expression are enhanced in high‑fat fed prepubertal female pigs by a diet including omega‑3 fatty acids and Bifdobacterium breve CECT8242
dc.creator.none.fl_str_mv Huguet, Gemma
Puig‑Parnau, Irene
Serrano Casasola, José Carlos Enrique
Martín Garí, Meritxell
Rodríguez Palmero, María
Moreno‑Muñoz, José Antonio
Tibau, Joan
Kádár, Elisabet
author Huguet, Gemma
author_facet Huguet, Gemma
Puig‑Parnau, Irene
Serrano Casasola, José Carlos Enrique
Martín Garí, Meritxell
Rodríguez Palmero, María
Moreno‑Muñoz, José Antonio
Tibau, Joan
Kádár, Elisabet
author_role author
author2 Puig‑Parnau, Irene
Serrano Casasola, José Carlos Enrique
Martín Garí, Meritxell
Rodríguez Palmero, María
Moreno‑Muñoz, José Antonio
Tibau, Joan
Kádár, Elisabet
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pig
Neurogenesis
Neural plasticity
Doublecortin
Arc
Hippocampus
topic Pig
Neurogenesis
Neural plasticity
Doublecortin
Arc
Hippocampus
description Purpose Obesity during childhood has become a pandemic disease, mainly caused by a diet rich in sugars and fatty acids. Among other negative effects, these diets can induce cognitive impairment and reduce neuroplasticity. It is well known that omega-3 and probiotics have a beneficial impact on health and cognition, and we have hypothesized that a diet enriched with Bifidobacterium breve and omega-3 could potentiate neuroplasticity in prepubertal pigs on a high-fat diet. Methods Young female piglets were fed during 10 weeks with: standard diet (T1), high-fat (HF) diet (T2), HF diet including B. breve CECT8242 (T3) and HF diet including the probiotic and omega-3 fatty acids (T4). Using hippocampal sections, we analyzed by immunocytochemistry the levels of doublecortin (DCX) to study neurogenesis, and activity-regulated cytoskeleton-associated protein (Arc) as a synaptic plasticity related protein. Results No effect of T2 or T3 was observed, whereas T4 increased both DCX+ cells and Arc expression. Therefore, a diet enriched with supplements of B. breve and omega-3 increases neurogenesis and synaptic plasticity in prepubertal females on a HF diet from nine weeks of age to sexual maturity. Furthermore, the analysis of serum cholesterol and HDL indicate that neurogenesis was related to lipidic demand in piglets fed with control or HF diets, but the neurogenic effect induced by the T4 diet was exerted by mechanisms independent of this lipidic demand. Conclusion Our results show that the T4 dietary treatment is effective in potentiating neural plasticity in the dorsal hippocampus of prepubertal females on a HF diet.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1007/s00394-023-03165-1
https://hdl.handle.net/10459.1/464194
url https://doi.org/10.1007/s00394-023-03165-1
https://hdl.handle.net/10459.1/464194
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.1007/s00394-023-03165-1
European Journal of Nutrition, 2023, vol. 62, p. 2463–2473
dc.rights.none.fl_str_mv cc-by (c) Gemma Huguet et al., 2023
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Gemma Huguet et al., 2023
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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