An all-in-one point-of-care testing device for multiplexed detection of respiratory infections

[EN] The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing ap...

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Detalhes bibliográficos
Autores: Teixeira, William|||0000-0002-6729-9508, Pallás-Tamarit, Yeray|||0000-0002-1167-5631, Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419, Carrascosa Rubio, Javier|||0009-0003-4615-5278, Maquieira, Angel|||0000-0003-4641-4957, Morais, Sergi|||0000-0002-3722-2358, Sena-Torralba, Amadeo, Gozalbo-Rovira, Roberto, Rodríguez-Díaz, Jesús, Navarro, David, Giménez-Romero, David
Tipo de documento: artigo
Data de publicação:2022
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositório:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglês
OAI Identifier:oai:riunet.upv.es:10251/192049
Acesso em linha:https://riunet.upv.es/handle/10251/192049
Access Level:Acceso aberto
Palavra-chave:COVID-19
SARS-CoV-2
Influenza
Adenovirus
Respiratory syncytial virus
Microfluidics
Immunoassay
QUIMICA ANALITICA
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
id ES_7ecb1559dbab1c76145a45aa1db56b60
oai_identifier_str oai:riunet.upv.es:10251/192049
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
title An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
spellingShingle An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
Teixeira, William|||0000-0002-6729-9508
COVID-19
SARS-CoV-2
Influenza
Adenovirus
Respiratory syncytial virus
Microfluidics
Immunoassay
QUIMICA ANALITICA
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
title_short An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
title_full An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
title_fullStr An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
title_full_unstemmed An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
title_sort An all-in-one point-of-care testing device for multiplexed detection of respiratory infections
dc.creator.none.fl_str_mv Teixeira, William|||0000-0002-6729-9508
Pallás-Tamarit, Yeray|||0000-0002-1167-5631
Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
Carrascosa Rubio, Javier|||0009-0003-4615-5278
Maquieira, Angel|||0000-0003-4641-4957
Morais, Sergi|||0000-0002-3722-2358
Sena-Torralba, Amadeo
Gozalbo-Rovira, Roberto
Rodríguez-Díaz, Jesús
Navarro, David
Giménez-Romero, David
author Teixeira, William|||0000-0002-6729-9508
author_facet Teixeira, William|||0000-0002-6729-9508
Pallás-Tamarit, Yeray|||0000-0002-1167-5631
Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
Carrascosa Rubio, Javier|||0009-0003-4615-5278
Maquieira, Angel|||0000-0003-4641-4957
Morais, Sergi|||0000-0002-3722-2358
Sena-Torralba, Amadeo
Gozalbo-Rovira, Roberto
Rodríguez-Díaz, Jesús
Navarro, David
Giménez-Romero, David
author_role author
author2 Pallás-Tamarit, Yeray|||0000-0002-1167-5631
Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419
Carrascosa Rubio, Javier|||0009-0003-4615-5278
Maquieira, Angel|||0000-0003-4641-4957
Morais, Sergi|||0000-0002-3722-2358
Sena-Torralba, Amadeo
Gozalbo-Rovira, Roberto
Rodríguez-Díaz, Jesús
Navarro, David
Giménez-Romero, David
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Escuela Técnica Superior de Ingeniería de Telecomunicación
Departamento de Comunicaciones
Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial
Departamento de Química
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Grupo de Medioambiente y Seguridad Industrial. MEDASEGI
Centro de Investigación e Innovación en Bioingeniería
GENERALITAT VALENCIANA
AGENCIA ESTATAL DE INVESTIGACION
European Regional Development Fund
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv COVID-19
SARS-CoV-2
Influenza
Adenovirus
Respiratory syncytial virus
Microfluidics
Immunoassay
QUIMICA ANALITICA
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
topic COVID-19
SARS-CoV-2
Influenza
Adenovirus
Respiratory syncytial virus
Microfluidics
Immunoassay
QUIMICA ANALITICA
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
description [EN] The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing approaches. Herein, we present a fast and robust all-in-one POCT device for parallel viral antigen and serological analysis. The biosensing approach consists of a functionalized polycarbonate disc-shaped surface with microfluidic structures, where specific bioreagents are immobilized in microarray format, and a portable optoelectronic analyzer. The biosensor quantifies the concentration of viral antigens and specific immunoglobulins G and M for SARS-CoV-2, influenza A/B, adenovirus, and respiratory syncytial virus, using 30¿¿L of a sample. The semi-automated analysis of 6 samples is performed in 30¿min. Validation studies performed with 135 serum samples and 147 nasopharyngeal specimens reveal high diagnostic sensitivity (98¿100%) and specificity (84¿98%), achieving an excellent agreement (¿¿=¿0.937) with commercial immunoassays, which complies with the World Health Organization criteria for POC COVID-19 diagnostic tests. The versatility of the POCT device paves the way for the detection of other pathogens and analytes in the incoming post-pandemic world, integrating specific bioreagents against different variants of concerns and interests.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/192049
url https://riunet.upv.es/handle/10251/192049
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-110713RB-I00 HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO
Generalitat Valenciana https://doi.org/10.13039/501100003359 COVID-19%2F5 ADAPTACION Y VALIDACION DE SISTEMAS BIOSENSORES CON TRANSDUCCIÓN OPTICA-CD Y VISCOELASTICA-QCM PARA LA DETECCION DE SARS
Universitat Politècnica de València https://doi.org/10.13039/501100004233 FPI 2017
Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-19-01-06
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2020%2F094 HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling An all-in-one point-of-care testing device for multiplexed detection of respiratory infectionsTeixeira, William|||0000-0002-6729-9508Pallás-Tamarit, Yeray|||0000-0002-1167-5631Juste-Dolz, Augusto Miguel|||0000-0003-4889-6419Carrascosa Rubio, Javier|||0009-0003-4615-5278Maquieira, Angel|||0000-0003-4641-4957Morais, Sergi|||0000-0002-3722-2358Sena-Torralba, AmadeoGozalbo-Rovira, RobertoRodríguez-Díaz, JesúsNavarro, DavidGiménez-Romero, DavidCOVID-19SARS-CoV-2InfluenzaAdenovirusRespiratory syncytial virusMicrofluidicsImmunoassayQUIMICA ANALITICA03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades[EN] The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing approaches. Herein, we present a fast and robust all-in-one POCT device for parallel viral antigen and serological analysis. The biosensing approach consists of a functionalized polycarbonate disc-shaped surface with microfluidic structures, where specific bioreagents are immobilized in microarray format, and a portable optoelectronic analyzer. The biosensor quantifies the concentration of viral antigens and specific immunoglobulins G and M for SARS-CoV-2, influenza A/B, adenovirus, and respiratory syncytial virus, using 30¿¿L of a sample. The semi-automated analysis of 6 samples is performed in 30¿min. Validation studies performed with 135 serum samples and 147 nasopharyngeal specimens reveal high diagnostic sensitivity (98¿100%) and specificity (84¿98%), achieving an excellent agreement (¿¿=¿0.937) with commercial immunoassays, which complies with the World Health Organization criteria for POC COVID-19 diagnostic tests. The versatility of the POCT device paves the way for the detection of other pathogens and analytes in the incoming post-pandemic world, integrating specific bioreagents against different variants of concerns and interests.W. T and A. J-D acknowledge the financial support for the Ph.D. studies (PAID-19-01-06 and FPI-UPV 2017 grants, respectively). S.M awards grant PID2019-110713RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe." A.M awards grant DOGV no. 8815, May 19, 2020, pp. 16712-16726. The research was also supported by the Generalitat Valenciana (PROMETEO/2020/094, Proyecto "88/2021"). The authors gratefully acknowledge the outstanding contribution of Alicia Parra, Violeta Espada, Roberto Renuncio, and Jose A. Marzal for collecting the nasopharyngeal swab samples from the Health Centre of the Universitat Politecnica de Valencia. We also gratefully thank Dr. L. Mira and G. Rios for carefully reading this manuscript and the four anonymous reviewers for their insightful comments and suggestions.ElsevierEscuela Técnica Superior de Ingeniería de TelecomunicaciónDepartamento de ComunicacionesEscuela Técnica Superior de Ingeniería Aeroespacial y Diseño IndustrialDepartamento de QuímicaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGrupo de Medioambiente y Seguridad Industrial. MEDASEGICentro de Investigación e Innovación en BioingenieríaGENERALITAT VALENCIANAAGENCIA ESTATAL DE INVESTIGACIONEuropean Regional Development FundUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/192049reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-110713RB-I00 HACIA EL BIOSENSADO HOLOGRAFICO AVANZADOGeneralitat Valenciana https://doi.org/10.13039/501100003359 COVID-19%2F5 ADAPTACION Y VALIDACION DE SISTEMAS BIOSENSORES CON TRANSDUCCIÓN OPTICA-CD Y VISCOELASTICA-QCM PARA LA DETECCION DE SARSUniversitat Politècnica de València https://doi.org/10.13039/501100004233 FPI 2017Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-19-01-06Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2020%2F094 HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADOopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1920492026-06-13T07:49:27Z
score 15,301603

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