Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks

Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However,...

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Autores: Crowe, James A., El-Tamer, A., Nagel, David, Koroleva, Anastasia V., Madrid-Wolff, Jorge, Olarte, Omar E., Sokolovsky, S., Estévez Priego, Estefanía, Ludl, Adriaan Alexander, Soriano i Fradera, Jordi, Loza Álvarez, Pablo, Chichkov, Boris N., Hill, Eric J., Parri, Harri R., Rafailov, Edik U.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/172345
Acceso en línea:https://hdl.handle.net/2445/172345
Access Level:acceso abierto
Palabra clave:Cèl·lules mare
Xarxes neuronals (Neurobiologia)
Sistemes complexos
Stem cells
Neural networks (Neurobiology)
Complex systems
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spelling Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networksCrowe, James A.El-Tamer, A.Nagel, DavidKoroleva, Anastasia V.Madrid-Wolff, JorgeOlarte, Omar E.Sokolovsky, S.Estévez Priego, EstefaníaLudl, Adriaan AlexanderSoriano i Fradera, JordiLoza Álvarez, PabloChichkov, Boris N.Hill, Eric J.Parri, Harri R.Rafailov, Edik U.Cèl·lules mareXarxes neuronals (Neurobiologia)Sistemes complexosStem cellsNeural networks (Neurobiology)Complex systemsRecent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing defined in vitro human neural networks for application in influencing neurite guidance and complex network activity.Royal Society of Chemistry2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/172345Articles publicats en revistes (Física de la Matèria Condensada)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1039/c9lc01209eLab on a Chip, 2020, vol. 20, p. 1792-1806https://doi.org/10.1039/c9lc01209einfo:eu-repo/grantAgreement/EC/H2020/654148info:eu-repo/grantAgreement/EC/H2020/713140info:eu-repo/grantAgreement/EC/H2020/851734cc by (c) Crowe et al., 2020http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1723452026-05-27T06:46:51Z
dc.title.none.fl_str_mv Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
title Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
spellingShingle Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
Crowe, James A.
Cèl·lules mare
Xarxes neuronals (Neurobiologia)
Sistemes complexos
Stem cells
Neural networks (Neurobiology)
Complex systems
title_short Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
title_full Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
title_fullStr Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
title_full_unstemmed Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
title_sort Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
dc.creator.none.fl_str_mv Crowe, James A.
El-Tamer, A.
Nagel, David
Koroleva, Anastasia V.
Madrid-Wolff, Jorge
Olarte, Omar E.
Sokolovsky, S.
Estévez Priego, Estefanía
Ludl, Adriaan Alexander
Soriano i Fradera, Jordi
Loza Álvarez, Pablo
Chichkov, Boris N.
Hill, Eric J.
Parri, Harri R.
Rafailov, Edik U.
author Crowe, James A.
author_facet Crowe, James A.
El-Tamer, A.
Nagel, David
Koroleva, Anastasia V.
Madrid-Wolff, Jorge
Olarte, Omar E.
Sokolovsky, S.
Estévez Priego, Estefanía
Ludl, Adriaan Alexander
Soriano i Fradera, Jordi
Loza Álvarez, Pablo
Chichkov, Boris N.
Hill, Eric J.
Parri, Harri R.
Rafailov, Edik U.
author_role author
author2 El-Tamer, A.
Nagel, David
Koroleva, Anastasia V.
Madrid-Wolff, Jorge
Olarte, Omar E.
Sokolovsky, S.
Estévez Priego, Estefanía
Ludl, Adriaan Alexander
Soriano i Fradera, Jordi
Loza Álvarez, Pablo
Chichkov, Boris N.
Hill, Eric J.
Parri, Harri R.
Rafailov, Edik U.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules mare
Xarxes neuronals (Neurobiologia)
Sistemes complexos
Stem cells
Neural networks (Neurobiology)
Complex systems
topic Cèl·lules mare
Xarxes neuronals (Neurobiologia)
Sistemes complexos
Stem cells
Neural networks (Neurobiology)
Complex systems
description Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing defined in vitro human neural networks for application in influencing neurite guidance and complex network activity.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/172345
url https://hdl.handle.net/2445/172345
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1039/c9lc01209e
Lab on a Chip, 2020, vol. 20, p. 1792-1806
https://doi.org/10.1039/c9lc01209e
info:eu-repo/grantAgreement/EC/H2020/654148
info:eu-repo/grantAgreement/EC/H2020/713140
info:eu-repo/grantAgreement/EC/H2020/851734
dc.rights.none.fl_str_mv cc by (c) Crowe et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Crowe et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Física de la Matèria Condensada)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,30478

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