Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)

Höfle, Ursula; Gonzalez-Lopez, Juan Jose; Camacho, Maria Cruz; Solà-Ginés, Marc; Moreno-Mingorance, Albert; Hernández, Jose Manuel; De La Puente, Javier; Pineda-Pampliega, Javier; Aguirre, José Ignacio; Torres-Medina, Fernando; Ramis, Antoni; Majó, Natalia; Blas, Julio; Migura-Garcia, Lourdes

Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)

White stork (Ciconia ciconia) may act as a reservoir and vehicle of cephalosporin resistant (CR) Escherichia coli. Between 2011 and 2014, we sampled white storks from colonies exposed to different degrees of anthropic pressure across the major areas of natural distribution of white storks in Spain....

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Authors:	Höfle, Ursula, Gonzalez-Lopez, Juan Jose, Camacho, Maria Cruz, Solà-Ginés, Marc, Moreno-Mingorance, Albert, Hernández, Jose Manuel, De La Puente, Javier, Pineda-Pampliega, Javier, Aguirre, José Ignacio, Torres-Medina, Fernando, Ramis, Antoni, Majó, Natalia, Blas, Julio, Migura-Garcia, Lourdes
Format:	article
Publication Date:	2020
Country:	España
Institution:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:20.500.12327/896
Online Access:	http://hdl.handle.net/20.500.12327/896 https://doi.org/10.3389/fmicb.2020.01397
Access Level:	Open access
Keyword:	619

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network_acronym_str	ES
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spelling	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)Höfle, UrsulaGonzalez-Lopez, Juan JoseCamacho, Maria CruzSolà-Ginés, MarcMoreno-Mingorance, AlbertHernández, Jose ManuelDe La Puente, JavierPineda-Pampliega, JavierAguirre, José IgnacioTorres-Medina, FernandoRamis, AntoniMajó, NataliaBlas, JulioMigura-Garcia, Lourdes619White stork (Ciconia ciconia) may act as a reservoir and vehicle of cephalosporin resistant (CR) Escherichia coli. Between 2011 and 2014, we sampled white storks from colonies exposed to different degrees of anthropic pressure across the major areas of natural distribution of white storks in Spain. Cloacal swab samples (n = 467) were obtained from individuals belonging to 12 different colonies from six different regions. Additionally, 70 samples were collected from recently deposited droppings at the base of nesting platforms. We phenotypically characterized E. coli isolates, confirmed presence of CR genes and classified plasmids. Risk factors for acquiring these genes were assessed. Overall, 8.8% (41 out of 467) storks carried CR E. coli in their cloaca and five (7.1%) were identified from recently deposited droppings; therefore, 46 isolates were further characterized. Of them, 20 contained blaCTX–M–1, nine blaCMY–2, six blaCTX–M–14, four blaSHV–12, three blaCTX–M–15, two blaCTX–M–32, one blaCTX–M–1 together with blaCMY–2, and one blaCTX–M–1 together with blaSHV–12. All were multidrug-resistant, and four harbored the plasmid-mediated colistin resistance mcr-1 gene. CR genes were associated with the presence of IncI1, IncFIB, and IncN replicon families. XbaI-macrorestriction analysis revealed a great diversity among most of the XbaI-PFGE types, but indistinguishable types were also seen with isolates obtained from different locations. Clonal complex 10 was the most common among CR E. coli and two blaCTX–M–15 positive isolates were identified as B2-ST131. Carriage of CR E. coli was significantly higher in colonies located close to solid urban waste disposal sites in which foraging on human waste was more likely and in one case to cattle grazing. The co-occurrence of blaCMY–2 and mcr-1 on plasmids of E. coli isolated from wild birds as early as 2011 is of note, as the earliest previous report of mcr-1 in wild birds is from 2016. Our study shows that foraging at landfills and in association with cattle grazing are important risk factors for the acquisition of CR E. coli in white storks.info:eu-repo/semantics/publishedVersionFrontiers MediaProducció AnimalSanitat Animal2020info:eu-repo/semantics/article13http://hdl.handle.net/20.500.12327/896https://doi.org/10.3389/fmicb.2020.01397reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in MicrobiologyMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095586-B-C22/OPTIMIZATION OF DIAGNOSIS AND TREATMENT OF POSTWEANING DIARRHEAS IN PIGS: TOWARDS A RATIONAL USE OF ANTIBIOTICS/MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL/INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2011‐00111‐C03-01/ES/Avian influenza: relationship between the host, the pathogen and the ecosystem/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/8962026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
title	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
spellingShingle	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia) Höfle, Ursula 619
title_short	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
title_full	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
title_fullStr	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
title_full_unstemmed	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
title_sort	Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)
dc.creator.none.fl_str_mv	Höfle, Ursula Gonzalez-Lopez, Juan Jose Camacho, Maria Cruz Solà-Ginés, Marc Moreno-Mingorance, Albert Hernández, Jose Manuel De La Puente, Javier Pineda-Pampliega, Javier Aguirre, José Ignacio Torres-Medina, Fernando Ramis, Antoni Majó, Natalia Blas, Julio Migura-Garcia, Lourdes
author	Höfle, Ursula
author_facet	Höfle, Ursula Gonzalez-Lopez, Juan Jose Camacho, Maria Cruz Solà-Ginés, Marc Moreno-Mingorance, Albert Hernández, Jose Manuel De La Puente, Javier Pineda-Pampliega, Javier Aguirre, José Ignacio Torres-Medina, Fernando Ramis, Antoni Majó, Natalia Blas, Julio Migura-Garcia, Lourdes
author_role	author
author2	Gonzalez-Lopez, Juan Jose Camacho, Maria Cruz Solà-Ginés, Marc Moreno-Mingorance, Albert Hernández, Jose Manuel De La Puente, Javier Pineda-Pampliega, Javier Aguirre, José Ignacio Torres-Medina, Fernando Ramis, Antoni Majó, Natalia Blas, Julio Migura-Garcia, Lourdes
author2_role	author author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Producció Animal Sanitat Animal
dc.subject.none.fl_str_mv	619
topic	619
description	White stork (Ciconia ciconia) may act as a reservoir and vehicle of cephalosporin resistant (CR) Escherichia coli. Between 2011 and 2014, we sampled white storks from colonies exposed to different degrees of anthropic pressure across the major areas of natural distribution of white storks in Spain. Cloacal swab samples (n = 467) were obtained from individuals belonging to 12 different colonies from six different regions. Additionally, 70 samples were collected from recently deposited droppings at the base of nesting platforms. We phenotypically characterized E. coli isolates, confirmed presence of CR genes and classified plasmids. Risk factors for acquiring these genes were assessed. Overall, 8.8% (41 out of 467) storks carried CR E. coli in their cloaca and five (7.1%) were identified from recently deposited droppings; therefore, 46 isolates were further characterized. Of them, 20 contained blaCTX–M–1, nine blaCMY–2, six blaCTX–M–14, four blaSHV–12, three blaCTX–M–15, two blaCTX–M–32, one blaCTX–M–1 together with blaCMY–2, and one blaCTX–M–1 together with blaSHV–12. All were multidrug-resistant, and four harbored the plasmid-mediated colistin resistance mcr-1 gene. CR genes were associated with the presence of IncI1, IncFIB, and IncN replicon families. XbaI-macrorestriction analysis revealed a great diversity among most of the XbaI-PFGE types, but indistinguishable types were also seen with isolates obtained from different locations. Clonal complex 10 was the most common among CR E. coli and two blaCTX–M–15 positive isolates were identified as B2-ST131. Carriage of CR E. coli was significantly higher in colonies located close to solid urban waste disposal sites in which foraging on human waste was more likely and in one case to cattle grazing. The co-occurrence of blaCMY–2 and mcr-1 on plasmids of E. coli isolated from wild birds as early as 2011 is of note, as the earliest previous report of mcr-1 in wild birds is from 2016. Our study shows that foraging at landfills and in association with cattle grazing are important risk factors for the acquisition of CR E. coli in white storks.
publishDate	2020
dc.date.none.fl_str_mv	2020
dc.type.none.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12327/896 https://doi.org/10.3389/fmicb.2020.01397
url	http://hdl.handle.net/20.500.12327/896 https://doi.org/10.3389/fmicb.2020.01397
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Frontiers in Microbiology MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095586-B-C22/OPTIMIZATION OF DIAGNOSIS AND TREATMENT OF POSTWEANING DIARRHEAS IN PIGS: TOWARDS A RATIONAL USE OF ANTIBIOTICS/ MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL/ INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2011‐00111‐C03-01/ES/Avian influenza: relationship between the host, the pathogen and the ecosystem/
dc.rights.none.fl_str_mv	Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	13
dc.publisher.none.fl_str_mv	Frontiers Media
publisher.none.fl_str_mv	Frontiers Media
dc.source.none.fl_str_mv	reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,812429

Foraging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)

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