Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma

Osteosarcoma (os) is the most common primary bone tumor but current therapies still have poor prognosis. Cold Atmospheric Plasma (CAP) and Plasma activated media (PAM) have shown potential to eliminate cancer cells in other tumors. It is thought that Reactive Oxygen and Nitrogen species (RONS) in PA...

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Autores: Tornin Cavielles, Juan|||0000-0002-7676-3958, Mateu Sanz, Miguel|||0000-0001-5117-6071, Rodríguez, Aida, Labay, Cédric Pierre|||0000-0001-5147-5100, Rodríguez, Rene, Canal Barnils, Cristina|||0000-0002-3039-7462
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/168001
Acceso en línea:https://hdl.handle.net/2117/168001
https://dx.doi.org/10.1038/s41598-019-47128-1
Access Level:acceso abierto
Palabra clave:Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
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spelling Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcomaTornin Cavielles, Juan|||0000-0002-7676-3958Mateu Sanz, Miguel|||0000-0001-5117-6071Rodríguez, AidaLabay, Cédric Pierre|||0000-0001-5147-5100Rodríguez, ReneCanal Barnils, Cristina|||0000-0002-3039-7462Biomedical materialsMaterials biomèdicsÀrees temàtiques de la UPC::Enginyeria dels materialsOsteosarcoma (os) is the most common primary bone tumor but current therapies still have poor prognosis. Cold Atmospheric Plasma (CAP) and Plasma activated media (PAM) have shown potential to eliminate cancer cells in other tumors. It is thought that Reactive Oxygen and Nitrogen species (RONS) in PAM are key players but cell culture media composition alters treatment outcomes and data interpretation due to scavenging of certain RONS. In this work, an atmospheric pressure plasma jet was employed to obtain PAM in the presence or absence of pyruvate and used to treat the SaOS-2 (OS) cell line or hBM-MSC healthy cells. OS cells show higher sensitivity to PAM treatment than healthy cells, both in medium with and without pyruvate, activating apoptosis, DNA damage and deregulating cellular pathways mediated by c-JUN, AKT, AMPK or STAT3. In line with previous works, lack of pyruvate increases cytotoxic potential of PAM affecting cancer and healthy cells by increasing 10–100 times the concentration of H2o2 without altering that of nitrites and thus decreasing CAP anti-tumor selectivity. Suitable conditions for CAP anti-cancer selectivity can be obtained by modifying plasma process parameters (distance, flow, treatment time) to obtain adequate balance of the different RONS in cell culture media.Nature20192019-07-2320192019-09-06journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/168001https://dx.doi.org/10.1038/s41598-019-47128-1reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 714793 Atmospheric Pressure plAsma meets biomaterials for bone Cancer HEalingopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1680012026-05-27T15:37:01Z
dc.title.none.fl_str_mv Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
title Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
spellingShingle Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
Tornin Cavielles, Juan|||0000-0002-7676-3958
Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
title_short Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
title_full Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
title_fullStr Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
title_full_unstemmed Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
title_sort Pyruvate plays a main role in the antitumoral selectivity of cold atmospheric plasma in osteosarcoma
dc.creator.none.fl_str_mv Tornin Cavielles, Juan|||0000-0002-7676-3958
Mateu Sanz, Miguel|||0000-0001-5117-6071
Rodríguez, Aida
Labay, Cédric Pierre|||0000-0001-5147-5100
Rodríguez, Rene
Canal Barnils, Cristina|||0000-0002-3039-7462
author Tornin Cavielles, Juan|||0000-0002-7676-3958
author_facet Tornin Cavielles, Juan|||0000-0002-7676-3958
Mateu Sanz, Miguel|||0000-0001-5117-6071
Rodríguez, Aida
Labay, Cédric Pierre|||0000-0001-5147-5100
Rodríguez, Rene
Canal Barnils, Cristina|||0000-0002-3039-7462
author_role author
author2 Mateu Sanz, Miguel|||0000-0001-5117-6071
Rodríguez, Aida
Labay, Cédric Pierre|||0000-0001-5147-5100
Rodríguez, Rene
Canal Barnils, Cristina|||0000-0002-3039-7462
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
topic Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria dels materials
description Osteosarcoma (os) is the most common primary bone tumor but current therapies still have poor prognosis. Cold Atmospheric Plasma (CAP) and Plasma activated media (PAM) have shown potential to eliminate cancer cells in other tumors. It is thought that Reactive Oxygen and Nitrogen species (RONS) in PAM are key players but cell culture media composition alters treatment outcomes and data interpretation due to scavenging of certain RONS. In this work, an atmospheric pressure plasma jet was employed to obtain PAM in the presence or absence of pyruvate and used to treat the SaOS-2 (OS) cell line or hBM-MSC healthy cells. OS cells show higher sensitivity to PAM treatment than healthy cells, both in medium with and without pyruvate, activating apoptosis, DNA damage and deregulating cellular pathways mediated by c-JUN, AKT, AMPK or STAT3. In line with previous works, lack of pyruvate increases cytotoxic potential of PAM affecting cancer and healthy cells by increasing 10–100 times the concentration of H2o2 without altering that of nitrites and thus decreasing CAP anti-tumor selectivity. Suitable conditions for CAP anti-cancer selectivity can be obtained by modifying plasma process parameters (distance, flow, treatment time) to obtain adequate balance of the different RONS in cell culture media.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-07-23
2019
2019-09-06
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/168001
https://dx.doi.org/10.1038/s41598-019-47128-1
url https://hdl.handle.net/2117/168001
https://dx.doi.org/10.1038/s41598-019-47128-1
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 714793 Atmospheric Pressure plAsma meets biomaterials for bone Cancer HEaling
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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