Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures

Complementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA surfaces to control network architecture is currently challen...

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Detalles Bibliográficos
Autores: Haeb, Anna-Christina, Yamamoto, Hideaki, Roach, Paul, Merryweather, Daniel, Sato, Y., Tornero, Daniel, Soriano i Fradera, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/228245
Acceso en línea:https://hdl.handle.net/2445/228245
Access Level:acceso abierto
Palabra clave:Sistemes microelectromecànics
Xarxes neuronals (Neurobiologia)
Microelectrònica
Microelectromechanical systems
Neural networks (Neurobiology)
Microelectronics
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spelling Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructuresHaeb, Anna-ChristinaYamamoto, HideakiRoach, PaulMerryweather, DanielSato, Y.Tornero, DanielSoriano i Fradera, JordiSistemes microelectromecànicsXarxes neuronals (Neurobiologia)MicroelectrònicaMicroelectromechanical systemsNeural networks (Neurobiology)MicroelectronicsComplementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA surfaces to control network architecture is currently challenging and platform specific. Here, we present a protocol for PDMS fabrication, HD-MEA chip preparation, PDMS-HD-MEA microstructure alignment, and cell culture, including alternatives. Our results show reproducible formation of modular networks with characteristic activity patterns across different systems. This protocol supports engineering of defined neuronal architectures while maintaining compatibility with various HD-MEA systems.Elsevier B.V.2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/228245Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.xpro.2026.104349STAR protocols, 2026, vol. 7, num.1https://doi.org/10.1016/j.xpro.2026.104349cc-by (c) Haeb, Anna-Christina, et al., 2026http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2282452026-05-27T06:46:51Z
dc.title.none.fl_str_mv Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
title Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
spellingShingle Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
Haeb, Anna-Christina
Sistemes microelectromecànics
Xarxes neuronals (Neurobiologia)
Microelectrònica
Microelectromechanical systems
Neural networks (Neurobiology)
Microelectronics
title_short Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
title_full Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
title_fullStr Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
title_full_unstemmed Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
title_sort Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures
dc.creator.none.fl_str_mv Haeb, Anna-Christina
Yamamoto, Hideaki
Roach, Paul
Merryweather, Daniel
Sato, Y.
Tornero, Daniel
Soriano i Fradera, Jordi
author Haeb, Anna-Christina
author_facet Haeb, Anna-Christina
Yamamoto, Hideaki
Roach, Paul
Merryweather, Daniel
Sato, Y.
Tornero, Daniel
Soriano i Fradera, Jordi
author_role author
author2 Yamamoto, Hideaki
Roach, Paul
Merryweather, Daniel
Sato, Y.
Tornero, Daniel
Soriano i Fradera, Jordi
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Sistemes microelectromecànics
Xarxes neuronals (Neurobiologia)
Microelectrònica
Microelectromechanical systems
Neural networks (Neurobiology)
Microelectronics
topic Sistemes microelectromecànics
Xarxes neuronals (Neurobiologia)
Microelectrònica
Microelectromechanical systems
Neural networks (Neurobiology)
Microelectronics
description Complementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA surfaces to control network architecture is currently challenging and platform specific. Here, we present a protocol for PDMS fabrication, HD-MEA chip preparation, PDMS-HD-MEA microstructure alignment, and cell culture, including alternatives. Our results show reproducible formation of modular networks with characteristic activity patterns across different systems. This protocol supports engineering of defined neuronal architectures while maintaining compatibility with various HD-MEA systems.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/228245
url https://hdl.handle.net/2445/228245
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.xpro.2026.104349
STAR protocols, 2026, vol. 7, num.1
https://doi.org/10.1016/j.xpro.2026.104349
dc.rights.none.fl_str_mv cc-by (c) Haeb, Anna-Christina, et al., 2026
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Haeb, Anna-Christina, et al., 2026
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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