Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies

Caveolins are a crucial component of plasma membrane (PM) caveolae but have also been localized to intracellular compartments, including the Golgi complex and lipid bodies. Mutant caveolins associated with human disease show aberrant trafficking to the PM and Golgi accumulation. We now show that the...

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Authors: Pol i Sorolla, Albert, Martin, Sally, Fernandez, Manuel A., Ingelmo-Torres, Mercedes, Ferguson, Charles, Enrich Bastús, Carles, Parton, Robert G.
Format: article
Status:Published version
Publication Date:2005
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/25242
Online Access:https://hdl.handle.net/2445/25242
Access Level:Open access
Keyword:Colesterol
Àcids grassos
Proteïnes de membrana
Aparell de Golgi
Cholesterol
Fatty acids
Membrane proteins
Golgi apparatus
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spelling Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid BodiesPol i Sorolla, AlbertMartin, SallyFernandez, Manuel A.Ingelmo-Torres, MercedesFerguson, CharlesEnrich Bastús, CarlesParton, Robert G.ColesterolÀcids grassosProteïnes de membranaAparell de GolgiCholesterolFatty acidsMembrane proteinsGolgi apparatusCaveolins are a crucial component of plasma membrane (PM) caveolae but have also been localized to intracellular compartments, including the Golgi complex and lipid bodies. Mutant caveolins associated with human disease show aberrant trafficking to the PM and Golgi accumulation. We now show that the Golgi pool of mainly newly synthesized protein is detergent-soluble and predominantly in a monomeric state, in contrast to the surface pool. Caveolin at the PM is not recognized by specific caveolin antibodies unless PM cholesterol is depleted. Exit from the Golgi complex of wild-type caveolin-1 or -3, but not vesicular stomatitis virus-G protein, is modulated by changing cellular cholesterol levels. In contrast, a muscular dystrophy-associated mutant of caveolin-3, Cav3P104L, showed increased accumulation in the Golgi complex upon cholesterol treatment. In addition, we demonstrate that in response to fatty acid treatment caveolin can follow a previously undescribed pathway from the PM to lipid bodies and can move from lipid bodies to the PM in response to removal of fatty acids. The results suggest that cholesterol is a rate-limiting component for caveolin trafficking. Changes in caveolin flux through the exocytic pathway can therefore be an indicator of cellular cholesterol and fatty acid levels.American Society for Cell Biology201220122005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfhttps://hdl.handle.net/2445/25242Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E04-08-0737Molecular Biology of the Cell, 2005, vol. 16, núm. 4, p. 2091-2105http://dx.doi.org/10.1091/mbc.E04-08-0737cc-by-nc-sa, (c) Pol et al., 2005http://creativecommons.org/licenses/cc-by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/252422026-05-29T05:05:01Z
dc.title.none.fl_str_mv Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
title Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
spellingShingle Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
Pol i Sorolla, Albert
Colesterol
Àcids grassos
Proteïnes de membrana
Aparell de Golgi
Cholesterol
Fatty acids
Membrane proteins
Golgi apparatus
title_short Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
title_full Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
title_fullStr Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
title_full_unstemmed Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
title_sort Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies
dc.creator.none.fl_str_mv Pol i Sorolla, Albert
Martin, Sally
Fernandez, Manuel A.
Ingelmo-Torres, Mercedes
Ferguson, Charles
Enrich Bastús, Carles
Parton, Robert G.
author Pol i Sorolla, Albert
author_facet Pol i Sorolla, Albert
Martin, Sally
Fernandez, Manuel A.
Ingelmo-Torres, Mercedes
Ferguson, Charles
Enrich Bastús, Carles
Parton, Robert G.
author_role author
author2 Martin, Sally
Fernandez, Manuel A.
Ingelmo-Torres, Mercedes
Ferguson, Charles
Enrich Bastús, Carles
Parton, Robert G.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Colesterol
Àcids grassos
Proteïnes de membrana
Aparell de Golgi
Cholesterol
Fatty acids
Membrane proteins
Golgi apparatus
topic Colesterol
Àcids grassos
Proteïnes de membrana
Aparell de Golgi
Cholesterol
Fatty acids
Membrane proteins
Golgi apparatus
description Caveolins are a crucial component of plasma membrane (PM) caveolae but have also been localized to intracellular compartments, including the Golgi complex and lipid bodies. Mutant caveolins associated with human disease show aberrant trafficking to the PM and Golgi accumulation. We now show that the Golgi pool of mainly newly synthesized protein is detergent-soluble and predominantly in a monomeric state, in contrast to the surface pool. Caveolin at the PM is not recognized by specific caveolin antibodies unless PM cholesterol is depleted. Exit from the Golgi complex of wild-type caveolin-1 or -3, but not vesicular stomatitis virus-G protein, is modulated by changing cellular cholesterol levels. In contrast, a muscular dystrophy-associated mutant of caveolin-3, Cav3P104L, showed increased accumulation in the Golgi complex upon cholesterol treatment. In addition, we demonstrate that in response to fatty acid treatment caveolin can follow a previously undescribed pathway from the PM to lipid bodies and can move from lipid bodies to the PM in response to removal of fatty acids. The results suggest that cholesterol is a rate-limiting component for caveolin trafficking. Changes in caveolin flux through the exocytic pathway can therefore be an indicator of cellular cholesterol and fatty acid levels.
publishDate 2005
dc.date.none.fl_str_mv 2005
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/25242
url https://hdl.handle.net/2445/25242
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E04-08-0737
Molecular Biology of the Cell, 2005, vol. 16, núm. 4, p. 2091-2105
http://dx.doi.org/10.1091/mbc.E04-08-0737
dc.rights.none.fl_str_mv cc-by-nc-sa, (c) Pol et al., 2005
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa, (c) Pol et al., 2005
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411752466186240
score 15,198674

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