Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes

We have analyzed the effects of minor compounds found in the unsaponifiable fraction (UF) and in the phenolic fraction (PF) of virgin olive oil (VOO) on LPS-induced inflammatory response via visfatin modulation in human monocytes. For this purpose, monocytes were incubated with UF and PF at differen...

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Detalhes bibliográficos
Autores: Martín Rubio, María Esther, Millán-Linares, María del Carmen, Naranjo, María C., Toscano Sánchez, María del Rocío, Abia González, María del Rocío, García Muriana, Francisco José, Bermúdez Pulgarín, Beatriz, Montserrat de la Paz, Sergio
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130760
Acesso em linha:https://hdl.handle.net/11441/130760
https://doi.org/10.1111/jfbc.12941
Access Level:Acceso aberto
Palavra-chave:NAMPT
Olive oil
Phenols
Unsaponifiable
Visfatin
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repository_id_str
spelling Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytesMartín Rubio, María EstherMillán-Linares, María del CarmenNaranjo, María C.Toscano Sánchez, María del RocíoAbia González, María del RocíoGarcía Muriana, Francisco JoséBermúdez Pulgarín, BeatrizMontserrat de la Paz, SergioNAMPTOlive oilPhenolsUnsaponifiableVisfatinWe have analyzed the effects of minor compounds found in the unsaponifiable fraction (UF) and in the phenolic fraction (PF) of virgin olive oil (VOO) on LPS-induced inflammatory response via visfatin modulation in human monocytes. For this purpose, monocytes were incubated with UF and PF at different concentrations and the pro-inflammatory stimulus LPS for 24 hr; squalene (SQ) and hydroxytyrosol (HTyr), the main components in UF and PF, respectively, were also used. The relative expression of both pro-inflammatory and anti-inflammatory genes, as well as other genes related to the NAD+-biosynthetic pathway was evaluated by RT-qPCR; and the secretion of some of these markers was assessed by ELISA procedures. We found that UF, SQ, PF, and HTyr prevented from LPS-induced dysfunctional gene expression and secretion via visfatin-related gene modulation in human monocytes. These findings unveil a potential beneficial role for minor compounds of VOO in the prevention of inflammatory-disorders. Practical application: In this project, potential health benefits of VOO micronutrients (unsaponifiable and phenolic compounds) were confirmed through anti-inflammatory assays. Our results reveal new interesting researching goals concerning nutrition by considering the role of bioactive VOO compounds in the prevention and progress of diseases related to inflammation.Wiley-BlackwellBiología CelularBioquímica Médica y Biología Molecular e Inmunología2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130760https://doi.org/10.1111/jfbc.12941reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Food Biochemistry, 43 (8), e12941.https://doi.org/10.1111/jfbc.12941info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1307602026-06-17T12:51:07Z
dc.title.none.fl_str_mv Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
title Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
spellingShingle Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
Martín Rubio, María Esther
NAMPT
Olive oil
Phenols
Unsaponifiable
Visfatin
title_short Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
title_full Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
title_fullStr Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
title_full_unstemmed Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
title_sort Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
dc.creator.none.fl_str_mv Martín Rubio, María Esther
Millán-Linares, María del Carmen
Naranjo, María C.
Toscano Sánchez, María del Rocío
Abia González, María del Rocío
García Muriana, Francisco José
Bermúdez Pulgarín, Beatriz
Montserrat de la Paz, Sergio
author Martín Rubio, María Esther
author_facet Martín Rubio, María Esther
Millán-Linares, María del Carmen
Naranjo, María C.
Toscano Sánchez, María del Rocío
Abia González, María del Rocío
García Muriana, Francisco José
Bermúdez Pulgarín, Beatriz
Montserrat de la Paz, Sergio
author_role author
author2 Millán-Linares, María del Carmen
Naranjo, María C.
Toscano Sánchez, María del Rocío
Abia González, María del Rocío
García Muriana, Francisco José
Bermúdez Pulgarín, Beatriz
Montserrat de la Paz, Sergio
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biología Celular
Bioquímica Médica y Biología Molecular e Inmunología
dc.subject.none.fl_str_mv NAMPT
Olive oil
Phenols
Unsaponifiable
Visfatin
topic NAMPT
Olive oil
Phenols
Unsaponifiable
Visfatin
description We have analyzed the effects of minor compounds found in the unsaponifiable fraction (UF) and in the phenolic fraction (PF) of virgin olive oil (VOO) on LPS-induced inflammatory response via visfatin modulation in human monocytes. For this purpose, monocytes were incubated with UF and PF at different concentrations and the pro-inflammatory stimulus LPS for 24 hr; squalene (SQ) and hydroxytyrosol (HTyr), the main components in UF and PF, respectively, were also used. The relative expression of both pro-inflammatory and anti-inflammatory genes, as well as other genes related to the NAD+-biosynthetic pathway was evaluated by RT-qPCR; and the secretion of some of these markers was assessed by ELISA procedures. We found that UF, SQ, PF, and HTyr prevented from LPS-induced dysfunctional gene expression and secretion via visfatin-related gene modulation in human monocytes. These findings unveil a potential beneficial role for minor compounds of VOO in the prevention of inflammatory-disorders. Practical application: In this project, potential health benefits of VOO micronutrients (unsaponifiable and phenolic compounds) were confirmed through anti-inflammatory assays. Our results reveal new interesting researching goals concerning nutrition by considering the role of bioactive VOO compounds in the prevention and progress of diseases related to inflammation.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130760
https://doi.org/10.1111/jfbc.12941
url https://hdl.handle.net/11441/130760
https://doi.org/10.1111/jfbc.12941
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Food Biochemistry, 43 (8), e12941.
https://doi.org/10.1111/jfbc.12941
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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