Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus

Aphids (Hemiptera: Aphididae) can harbor two types of bacterial symbionts. In addition to the obligate endosymbiont Buchnera aphidicola Munson, Baumann and Kinsey 1991 (Enterobacteriales: Enterobacteriaceae), several facultative symbiotic bacteria, called secondary (S) symbionts, have been identifie...

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Autores: Zouari, Sana, Ben Halima, Monia Kamel, Reyes-Prieto, Mariana, Latorre, Amparo, Gil, Rosario
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/184377
Acesso em linha:http://hdl.handle.net/10261/184377
Access Level:acceso abierto
Palavra-chave:Hyalopterus
Parasitoid
Secondary symbiont
Arsenophonus
Hamiltonella defensa
APSE
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spelling Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus HyalopterusZouari, SanaBen Halima, Monia KamelReyes-Prieto, MarianaLatorre, AmparoGil, RosarioHyalopterusParasitoidSecondary symbiontArsenophonusHamiltonella defensaAPSEAphids (Hemiptera: Aphididae) can harbor two types of bacterial symbionts. In addition to the obligate endosymbiont Buchnera aphidicola Munson, Baumann and Kinsey 1991 (Enterobacteriales: Enterobacteriaceae), several facultative symbiotic bacteria, called secondary (S) symbionts, have been identified among many important pest aphid species. To determine interpopulational diversity of S-symbionts, we carried out a survey in a total of 18 populations of six aphid species collected from six localities in Tunisia, by performing a diagnostic polymerase chain reaction analysis of partial 16S-23S rRNA operon sequences. While 61.7% of individuals contained only Buchnera, three S-symbionts were found at different frequencies. Arsenophonus sp. Gherna et al. 1991 (Enterobacteriales: Enterobacteriaceae) was found in all species under study except for Acyrtosiphon pisum (Harris 1776) (Aphidinae: Macrosiphini); Serratia symbiotica Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was present in all analyzed individuals of A. pisum but only sporadically in Aphis spiraecola (Patch 1914) (Aphidinae: Aphidini) and Hyalopterus amygdali (Blanchard 1840) (Aphidinae: Aphidini), while Hamiltonella defensa Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was found in all analyzed individuals of one population of Aphis gossypii (Glover 1877) (Aphidinae: Aphidini) and sporadically in two populations of Hyalopterus. The lysogenic bacteriophage APSE-1 (A. pisum secondary endosymbiont, type 1) was detected in the three populations infected with H. defensa. This bacteriophage has been associated with moderate protection against braconid parasitoids in pea aphids. The high prevalence of Arsenophonus sp. in our samples is in accordance with previous studies indicating that, among gammaproteobacteria, this genus is one of the most widespread insect facultative symbionts.This work was supported by grants BFU2015-64322-C2-1-R (co-financed by FEDER funds and Ministerio de Economía y Competitividad, Spain), PrometeoII/2014/065 (Conselleria d’Educació, Generalitat Valenciana, Spain). S. Zouari is a recipient of a fellowship from Tunisian Ministry of Higher Education, and a grant from the Tunisian-Algerian project 1521ICH/RO, 2012 (co-financed by Tunisian and Algerian Ministries of Higher Education). M. Reyes-Prieto is a recipient of a fellowship (number 538243) from Consejo Nacional de Ciencia y Tecnologia, México.Peer reviewedOxford University PressMinisterio de Economía y Competitividad (España)European CommissionGeneralitat ValencianaConsejo Nacional de Ciencia y Tecnología (México)Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/184377reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64322-C2-1-Rhttps://doi.org/10.1093/ee/nvy005Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1843772026-05-22T06:33:51Z
dc.title.none.fl_str_mv Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
title Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
spellingShingle Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
Zouari, Sana
Hyalopterus
Parasitoid
Secondary symbiont
Arsenophonus
Hamiltonella defensa
APSE
title_short Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
title_full Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
title_fullStr Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
title_full_unstemmed Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
title_sort Natural occurrence of secondary bacterial symbionts in aphids from Tunisia, with a focus on genus Hyalopterus
dc.creator.none.fl_str_mv Zouari, Sana
Ben Halima, Monia Kamel
Reyes-Prieto, Mariana
Latorre, Amparo
Gil, Rosario
author Zouari, Sana
author_facet Zouari, Sana
Ben Halima, Monia Kamel
Reyes-Prieto, Mariana
Latorre, Amparo
Gil, Rosario
author_role author
author2 Ben Halima, Monia Kamel
Reyes-Prieto, Mariana
Latorre, Amparo
Gil, Rosario
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Generalitat Valenciana
Consejo Nacional de Ciencia y Tecnología (México)
Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Hyalopterus
Parasitoid
Secondary symbiont
Arsenophonus
Hamiltonella defensa
APSE
topic Hyalopterus
Parasitoid
Secondary symbiont
Arsenophonus
Hamiltonella defensa
APSE
description Aphids (Hemiptera: Aphididae) can harbor two types of bacterial symbionts. In addition to the obligate endosymbiont Buchnera aphidicola Munson, Baumann and Kinsey 1991 (Enterobacteriales: Enterobacteriaceae), several facultative symbiotic bacteria, called secondary (S) symbionts, have been identified among many important pest aphid species. To determine interpopulational diversity of S-symbionts, we carried out a survey in a total of 18 populations of six aphid species collected from six localities in Tunisia, by performing a diagnostic polymerase chain reaction analysis of partial 16S-23S rRNA operon sequences. While 61.7% of individuals contained only Buchnera, three S-symbionts were found at different frequencies. Arsenophonus sp. Gherna et al. 1991 (Enterobacteriales: Enterobacteriaceae) was found in all species under study except for Acyrtosiphon pisum (Harris 1776) (Aphidinae: Macrosiphini); Serratia symbiotica Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was present in all analyzed individuals of A. pisum but only sporadically in Aphis spiraecola (Patch 1914) (Aphidinae: Aphidini) and Hyalopterus amygdali (Blanchard 1840) (Aphidinae: Aphidini), while Hamiltonella defensa Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was found in all analyzed individuals of one population of Aphis gossypii (Glover 1877) (Aphidinae: Aphidini) and sporadically in two populations of Hyalopterus. The lysogenic bacteriophage APSE-1 (A. pisum secondary endosymbiont, type 1) was detected in the three populations infected with H. defensa. This bacteriophage has been associated with moderate protection against braconid parasitoids in pea aphids. The high prevalence of Arsenophonus sp. in our samples is in accordance with previous studies indicating that, among gammaproteobacteria, this genus is one of the most widespread insect facultative symbionts.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/184377
url http://hdl.handle.net/10261/184377
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-64322-C2-1-R
https://doi.org/10.1093/ee/nvy005
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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