Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana

Guazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic gua...

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Autores: Atanasov, Kostadin Evgeniev, Barboza-Barquero, Luis, Fernández Tiburcio, Antonio, Alcázar Hernández, Rubén
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/138519
Acceso en línea:https://hdl.handle.net/2445/138519
Access Level:acceso abierto
Palabra clave:Arabidopsis thaliana
Poliamines
Genètica de poblacions
Polyamines
Population Genetics
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spelling Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thalianaAtanasov, Kostadin EvgenievBarboza-Barquero, LuisFernández Tiburcio, AntonioAlcázar Hernández, RubénArabidopsis thalianaPoliaminesGenètica de poblacionsArabidopsis thalianaPolyaminesPopulation GeneticsGuazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis of independent clh1-2, clh1-3, clh2-3, clh2-2 and double clh1-2 clh2-3 mutant alleles indicated that CLH1 and/or CLH2 loss-of-function or expression down-regulation promote guazatine tolerance in Arabidopsis. We report a natural mechanism by which Arabidopsis populations can overcome toxicity by the fungicide guazatine.Frontiers Media2019201920162019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/138519Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fpls.2016.00401Frontiers in Plant Science, 2016, vol. 7, p. 401https://doi.org/10.3389/fpls.2016.00401info:eu-repo/grantAgreement/EC/FP7/303568cc-by (c) Atanasov, Kostadin Evgeniev et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1385192026-05-29T05:05:01Z
dc.title.none.fl_str_mv Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
title Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
spellingShingle Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
Atanasov, Kostadin Evgeniev
Arabidopsis thaliana
Poliamines
Genètica de poblacions
Arabidopsis thaliana
Polyamines
Population Genetics
title_short Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
title_full Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
title_fullStr Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
title_full_unstemmed Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
title_sort Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
dc.creator.none.fl_str_mv Atanasov, Kostadin Evgeniev
Barboza-Barquero, Luis
Fernández Tiburcio, Antonio
Alcázar Hernández, Rubén
author Atanasov, Kostadin Evgeniev
author_facet Atanasov, Kostadin Evgeniev
Barboza-Barquero, Luis
Fernández Tiburcio, Antonio
Alcázar Hernández, Rubén
author_role author
author2 Barboza-Barquero, Luis
Fernández Tiburcio, Antonio
Alcázar Hernández, Rubén
author2_role author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Poliamines
Genètica de poblacions
Arabidopsis thaliana
Polyamines
Population Genetics
topic Arabidopsis thaliana
Poliamines
Genètica de poblacions
Arabidopsis thaliana
Polyamines
Population Genetics
description Guazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis of independent clh1-2, clh1-3, clh2-3, clh2-2 and double clh1-2 clh2-3 mutant alleles indicated that CLH1 and/or CLH2 loss-of-function or expression down-regulation promote guazatine tolerance in Arabidopsis. We report a natural mechanism by which Arabidopsis populations can overcome toxicity by the fungicide guazatine.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/138519
url https://hdl.handle.net/2445/138519
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fpls.2016.00401
Frontiers in Plant Science, 2016, vol. 7, p. 401
https://doi.org/10.3389/fpls.2016.00401
info:eu-repo/grantAgreement/EC/FP7/303568
dc.rights.none.fl_str_mv cc-by (c) Atanasov, Kostadin Evgeniev et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Atanasov, Kostadin Evgeniev et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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