Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii

11 pages, 6 figures.-- PMID: 19251887 [PubMed].-- Printed version published Apr 2009.

Bibliographic Details
Authors: Belloch, Carmela, Pérez-Torrado, Roberto, González, Sara S., Pérez Ortín, José E., García-Martínez, José, Querol, Amparo, Barrio, Eladio
Format: article
Publication Date:2009
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/13552
Online Access:http://hdl.handle.net/10261/13552
Access Level:Open access
Keyword:Natural hybrids
Saccharomyces cerevisiae
Saccharomyces kudriavzevii
Genome hybridization
Aneuploidy
aCGH
qRT-PCR
Recombination points
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spelling Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzeviiBelloch, CarmelaPérez-Torrado, RobertoGonzález, Sara S.Pérez Ortín, José E.García-Martínez, JoséQuerol, AmparoBarrio, EladioNatural hybridsSaccharomyces cerevisiaeSaccharomyces kudriavzeviiGenome hybridizationAneuploidyaCGHqRT-PCRRecombination points11 pages, 6 figures.-- PMID: 19251887 [PubMed].-- Printed version published Apr 2009.Recently, a new type of hybrid resulting from the hybridization between Saccharomyces cerevisiae and Saccharomyces kudriavzevii was described. These strains exhibit physiological properties of potential biotechnological interest. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. We characterized the genomic constitution of several wine S. cerevisiae S. kudriavzevii strains by using a combined approach based on the restriction fragment length polymorphism analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis, and gene dose determination by quantitative real-time PCR. The high similarity in the genome structures of the S. cerevisiae X S. kudriavzevii hybrids under study indicates that they originated from a single hybridization event. After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimeric chromosomes by the nonreciprocal recombination between homeologous chromosomes. These nonreciprocal recombinations between homeologous chromosomes occurred in highly conserved regions, such as Ty long terminal repeats (LTRs), rRNA regions, and conserved protein-coding genes. This study supports the hypothesis that chimeric chromosomes may have been generated by a mechanism similar to the recombination-mediated chromosome loss acting during meiosis in Saccharomyces hybrids. As a result of the selective processes acting during fermentation, hybrid genomes maintained the S. cerevisiae genome but reduced the S. kudriavzevii fraction.CICYT grants AGL2006-12703-CO2-01/ALI and -02/ALI from the Spanish Government to A.Q. and E.B., respectively. C.B. gratefully acknowledges an I3P postdoctoral fellowship from the Spanish Research Council (CSIC).Peer reviewedAmerican Society for Microbiology200920092009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501590574 bytesapplication/pdfhttp://hdl.handle.net/10261/13552reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/AEM.02282-08info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/135522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
title Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
spellingShingle Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
Belloch, Carmela
Natural hybrids
Saccharomyces cerevisiae
Saccharomyces kudriavzevii
Genome hybridization
Aneuploidy
aCGH
qRT-PCR
Recombination points
title_short Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
title_full Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
title_fullStr Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
title_full_unstemmed Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
title_sort Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
dc.creator.none.fl_str_mv Belloch, Carmela
Pérez-Torrado, Roberto
González, Sara S.
Pérez Ortín, José E.
García-Martínez, José
Querol, Amparo
Barrio, Eladio
author Belloch, Carmela
author_facet Belloch, Carmela
Pérez-Torrado, Roberto
González, Sara S.
Pérez Ortín, José E.
García-Martínez, José
Querol, Amparo
Barrio, Eladio
author_role author
author2 Pérez-Torrado, Roberto
González, Sara S.
Pérez Ortín, José E.
García-Martínez, José
Querol, Amparo
Barrio, Eladio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Natural hybrids
Saccharomyces cerevisiae
Saccharomyces kudriavzevii
Genome hybridization
Aneuploidy
aCGH
qRT-PCR
Recombination points
topic Natural hybrids
Saccharomyces cerevisiae
Saccharomyces kudriavzevii
Genome hybridization
Aneuploidy
aCGH
qRT-PCR
Recombination points
description 11 pages, 6 figures.-- PMID: 19251887 [PubMed].-- Printed version published Apr 2009.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/13552
url http://hdl.handle.net/10261/13552
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/AEM.02282-08
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 590574 bytes
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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