Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor

[EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Tran...

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Detalles Bibliográficos
Autores: Sola Landa, Alberto, Rodríguez García, Antonio, Amin, Rafat, Wohlleben, Wolfgang, Martín Martín, Juan Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/17970
Acceso en línea:https://academic.oup.com/nar/article/41/3/1767/2902433
https://hdl.handle.net/10612/17970
Access Level:acceso abierto
Palabra clave:Biología
Phosphate regulation
Nitrogen regulation
Streptomyces coelicolor
PhoR-PhoP
GlnR
PHO boxes
EMSA
DNase footprinting
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
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oai_identifier_str oai:buleria.unileon.es:10612/17970
network_acronym_str ES
network_name_str España
repository_id_str
spelling Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolorSola Landa, AlbertoRodríguez García, AntonioAmin, RafatWohlleben, WolfgangMartín Martín, Juan FranciscoBiologíaPhosphate regulationNitrogen regulationStreptomyces coelicolorPhoR-PhoPGlnRPHO boxesEMSADNase footprinting2415.01 Biología Molecular de Microorganismos2414.02 Fisiología Bacteriana[EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibriumSIERA-NET SysMO Project (STREAM) [GEN2006-27745-E/SYS to J.F.M. and FKZ 0315003 to W.W.]; Spanish Comisión Interministerial de Ciencia y Tecnología (CICYT) [BIO2010-16094]. Funding for open access charge: CICYT [BIO2010-16094].Oxford University PressMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://academic.oup.com/nar/article/41/3/1767/2902433https://hdl.handle.net/10612/17970reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/EU//GEN2006-27745-Einfo:eu-repo/grantAgreement/CICYT//BIO2010-16094info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/179702026-06-24T12:43:27Z
dc.title.none.fl_str_mv Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
title Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
spellingShingle Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
Sola Landa, Alberto
Biología
Phosphate regulation
Nitrogen regulation
Streptomyces coelicolor
PhoR-PhoP
GlnR
PHO boxes
EMSA
DNase footprinting
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
title_short Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
title_full Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
title_fullStr Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
title_full_unstemmed Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
title_sort Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
dc.creator.none.fl_str_mv Sola Landa, Alberto
Rodríguez García, Antonio
Amin, Rafat
Wohlleben, Wolfgang
Martín Martín, Juan Francisco
author Sola Landa, Alberto
author_facet Sola Landa, Alberto
Rodríguez García, Antonio
Amin, Rafat
Wohlleben, Wolfgang
Martín Martín, Juan Francisco
author_role author
author2 Rodríguez García, Antonio
Amin, Rafat
Wohlleben, Wolfgang
Martín Martín, Juan Francisco
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Phosphate regulation
Nitrogen regulation
Streptomyces coelicolor
PhoR-PhoP
GlnR
PHO boxes
EMSA
DNase footprinting
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
topic Biología
Phosphate regulation
Nitrogen regulation
Streptomyces coelicolor
PhoR-PhoP
GlnR
PHO boxes
EMSA
DNase footprinting
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
description [EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibrium
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://academic.oup.com/nar/article/41/3/1767/2902433
https://hdl.handle.net/10612/17970
url https://academic.oup.com/nar/article/41/3/1767/2902433
https://hdl.handle.net/10612/17970
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/EU//GEN2006-27745-E
info:eu-repo/grantAgreement/CICYT//BIO2010-16094
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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