Further molecular diagnosis determines lack of evidence for real seed transmission of tomato leaf curl New Delhi virus in cucurbits
[EN] Begomoviruses (family Geminiviridae) cause serious diseases in many crop families. Since 2013, the Spanish isolate of Tomato leaf curl New Delhi virus (ToLCNDV) has been a limiting factor for cucurbit production in the Mediterranean basin, forcing farmers to adopt new management and control str...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/205277 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/205277 |
| Access Level: | acceso abierto |
| Palabra clave: | Seed transmission Begomovirus ToLCNDV Cucurbit seedlings QPCR Rolling-circle amplification GENETICA PRODUCCION VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible 08.- Fomentar el crecimiento económico sostenido, inclusivo y sostenible, el empleo pleno y productivo, y el trabajo decente para todos 12.- Garantizar las pautas de consumo y de producción sostenibles |
| Sumario: | [EN] Begomoviruses (family Geminiviridae) cause serious diseases in many crop families. Since 2013, the Spanish isolate of Tomato leaf curl New Delhi virus (ToLCNDV) has been a limiting factor for cucurbit production in the Mediterranean basin, forcing farmers to adopt new management and control strategies. Although it is well known that begomoviruses are naturally transmitted by the whitefly Bemisia tabaci, the capacity of these viruses to be vertically transmitted through seeds remains controversial. Clarifying the potential ToLCNDV seed transmission is essential to understand the epidemiology of this threatening virus for cucurbits and to design appropriate control strategies. We assessed ToLCNDV distribution in the leaves, flowers, and seeds of infected plants of susceptible Cucumis melo accessions and tolerant genotypes of Cucurbita moschata by conventional and quantitative PCR, and analyzed whether the viral particle was transmitted to offspring. We also evaluated ToLCNDV presence in commercial seeds of cucurbits — zucchini (Cucurbita pepo), melon (C. melo), cucumber (Cucumis sativus), and watermelon (Citrullus lanatus) — and in their progenies. As the assayed seedlings remained symptomless, we increased the reliability and accuracy of detection by searching for replicative forms of ToLCNDV, combining Southern blot hybridization and rolling-circle amplification (RCA). However, integral genomic DNA was not identified in offspring plants. Although the seedborne nature of ToLCNDV was confirmed, our results do not support the transmission of this virus from contaminated seeds to progeny. |
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