Brown adipose tissue-specific insulin receptor knockout shows diabetic phenotype without insulin resistance.

Although insulin regulates metabolism in both brown and white adipocytes, the role of these tissues in energy storage and utilization is quite different. Recombination technology using the Cre-loxP approach allows inactivation of the insulin receptor in a tissue-specific manner. Mice lacking insulin...

Descripción completa

Detalles Bibliográficos
Autores: Guerra, C, Navarro, P, Valverde, A M, Arribas, M, Brüning, J, Kozak, L P, Kahn, C R, Benito, M
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/23090
Acceso en línea:https://hdl.handle.net/20.500.12105/23090
Access Level:acceso abierto
Palabra clave:Adipose Tissue, Brown
Animals
Diabetes Mellitus, Type 2
Female
Insulin
Insulin Resistance
Insulin Secretion
Male
Mice
Mice, Knockout
Mice, Transgenic
Phenotype
RNA
Receptor, Insulin
Signal Transduction
Tissue Distribution
Descripción
Sumario:Although insulin regulates metabolism in both brown and white adipocytes, the role of these tissues in energy storage and utilization is quite different. Recombination technology using the Cre-loxP approach allows inactivation of the insulin receptor in a tissue-specific manner. Mice lacking insulin receptors in brown adipocytes show an age-dependent loss of interscapular brown fat but increased expression of uncoupling protein-1 and -2. In parallel, these mice develop an insulin-secretion defect resulting in a progressive glucose intolerance, without insulin resistance. This model provides direct evidence for not only a role for the insulin receptors in brown fat adipogenesis, the data also suggest a novel role of brown adipose tissue in the regulation of insulin secretion and glucose homeostasis.