Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers

The extensive use of antibiotics in human and veterinary medicine and their subsequent release into the environment may have direct consequences for autochthonous bacterial communities, especially in freshwater ecosystems. In small streams and rivers, local inputs of wastewater treatment plants (WWT...

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Autores: Proia, Lorenzo, Von Schiller, Daniel, Sànchez Melsió, Alexandre, Sabater, Sergi, Borrego i Moré, Carles, Rodríguez Mozaz, Sara, Balcázar, José Luis
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/25464
Acceso en línea:http://hdl.handle.net/10256/25464
Access Level:acceso abierto
Palabra clave:Biofilms
Contaminants emergents en l'aigua
Emerging contaminants in water
Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Plantes de tractament
Sewage disposal plants
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spelling Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small riversProia, LorenzoVon Schiller, DanielSànchez Melsió, AlexandreSabater, SergiBorrego i Moré, CarlesRodríguez Mozaz, SaraBalcázar, José LuisBiofilmsContaminants emergents en l'aiguaEmerging contaminants in waterMedicaments -- Aspectes ambientalsDrugs -- Environmental aspectsAigües residuals -- Plantes de tractamentSewage disposal plantsThe extensive use of antibiotics in human and veterinary medicine and their subsequent release into the environment may have direct consequences for autochthonous bacterial communities, especially in freshwater ecosystems. In small streams and rivers, local inputs of wastewater treatment plants (WWTPs) may become important sources of organic matter, nutrients and emerging pollutants, such as antibiotic resistance genes (ARGs). In this study, we evaluated the effect of WWTP effluents as a source of ARGs in river biofilms. The prevalence of genes conferring resistance to main antibiotic families, such as beta-lactams (blaCTX-M), fluoroquinolones (qnrS), sulfonamides (sul I), and macrolides (ermB), was determined using quantitative PCR (qPCR) in biofilm samples collected upstream and downstream WWTPs discharge points in four low-order streams. Our results showed that the WWTP effluents strongly modified the hydrology, physico-chemistry and biological characteristics of the receiving streams and favoured the persistence and spread of antibiotic resistance in microbial benthic communities. It was also shown that the magnitude of effects depended on the relative contribution of each WWTP to the receiving system. Specifically, low concentrations of ARGs were detected at sites located upstream of the WWTPs, while a significant increase of their concentrations was observed in biofilms collected downstream of the WWTP discharge points (particularly ermB and sul I genes). These findings suggest that WWTP discharges may favour the increase and spread of antibiotic resistance among streambed biofilms. The present study also showed that the presence of ARGs in biofilms was noticeable far downstream of the WWTP discharge (up to 1 km). It is therefore reasonable to assume that biofilms may represent an ideal setting for the acquisition and spread of antibiotic resistance determinants and thus be considered suitable biological indicators of anthropogenic pollution by active pharmaceutical compoundsThis study has been supported by the European Union through the European Regional Development Fund (FEDER) and the Generalitat de Catalunya (Consolidated Research Group: Catalan Institute for Water Research 2014 SGR 291 ). J.L.B. acknowledges the Ramon y Cajal research fellowship ( RYC-2011-08154 ) from the Spanish Ministry of Economy and Competitiveness6Elsevierinfo2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/25464http://hdl.handle.net/10256/25464© Environmental Pollution, 2016, vol. 210, p. 121-128Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2015.11.035info:eu-repo/semantics/altIdentifier/issn/0269-7491info:eu-repo/semantics/altIdentifier/eissn/1873-6424Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/254642026-05-29T05:05:01Z
dc.title.none.fl_str_mv Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
title Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
spellingShingle Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
Proia, Lorenzo
Biofilms
Contaminants emergents en l'aigua
Emerging contaminants in water
Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Plantes de tractament
Sewage disposal plants
title_short Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
title_full Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
title_fullStr Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
title_full_unstemmed Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
title_sort Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
dc.creator.none.fl_str_mv Proia, Lorenzo
Von Schiller, Daniel
Sànchez Melsió, Alexandre
Sabater, Sergi
Borrego i Moré, Carles
Rodríguez Mozaz, Sara
Balcázar, José Luis
author Proia, Lorenzo
author_facet Proia, Lorenzo
Von Schiller, Daniel
Sànchez Melsió, Alexandre
Sabater, Sergi
Borrego i Moré, Carles
Rodríguez Mozaz, Sara
Balcázar, José Luis
author_role author
author2 Von Schiller, Daniel
Sànchez Melsió, Alexandre
Sabater, Sergi
Borrego i Moré, Carles
Rodríguez Mozaz, Sara
Balcázar, José Luis
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biofilms
Contaminants emergents en l'aigua
Emerging contaminants in water
Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Plantes de tractament
Sewage disposal plants
topic Biofilms
Contaminants emergents en l'aigua
Emerging contaminants in water
Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Plantes de tractament
Sewage disposal plants
description The extensive use of antibiotics in human and veterinary medicine and their subsequent release into the environment may have direct consequences for autochthonous bacterial communities, especially in freshwater ecosystems. In small streams and rivers, local inputs of wastewater treatment plants (WWTPs) may become important sources of organic matter, nutrients and emerging pollutants, such as antibiotic resistance genes (ARGs). In this study, we evaluated the effect of WWTP effluents as a source of ARGs in river biofilms. The prevalence of genes conferring resistance to main antibiotic families, such as beta-lactams (blaCTX-M), fluoroquinolones (qnrS), sulfonamides (sul I), and macrolides (ermB), was determined using quantitative PCR (qPCR) in biofilm samples collected upstream and downstream WWTPs discharge points in four low-order streams. Our results showed that the WWTP effluents strongly modified the hydrology, physico-chemistry and biological characteristics of the receiving streams and favoured the persistence and spread of antibiotic resistance in microbial benthic communities. It was also shown that the magnitude of effects depended on the relative contribution of each WWTP to the receiving system. Specifically, low concentrations of ARGs were detected at sites located upstream of the WWTPs, while a significant increase of their concentrations was observed in biofilms collected downstream of the WWTP discharge points (particularly ermB and sul I genes). These findings suggest that WWTP discharges may favour the increase and spread of antibiotic resistance among streambed biofilms. The present study also showed that the presence of ARGs in biofilms was noticeable far downstream of the WWTP discharge (up to 1 km). It is therefore reasonable to assume that biofilms may represent an ideal setting for the acquisition and spread of antibiotic resistance determinants and thus be considered suitable biological indicators of anthropogenic pollution by active pharmaceutical compounds
publishDate 2016
dc.date.none.fl_str_mv 2016
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer-reviewed
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/25464
http://hdl.handle.net/10256/25464
url http://hdl.handle.net/10256/25464
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2015.11.035
info:eu-repo/semantics/altIdentifier/issn/0269-7491
info:eu-repo/semantics/altIdentifier/eissn/1873-6424
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Environmental Pollution, 2016, vol. 210, p. 121-128
Articles publicats (D-B)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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