Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis

Four nr1j1 gene sequences were recently reported in the marine mussel Mytilus galloprovincialis. These genes are orthologs of the nuclear receptor family NR1I found in vertebrates, whose members like the pregnane x receptor (PXR) are known to play a major role in the regulation of xenobiotic metabol...

Descripción completa

Detalles Bibliográficos
Autores: Rodrigues, Juliana, Plata Calzado, Cristina, Gómez Román, María Paula, Boo, Sergio, Fonseca, Elza, Araújo, Mario, Vasconcelos, Vitor, Prieto Ortega, Ana Isabel, Jos Gallego, Ángeles Mencía, Campos, Alexandre
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/182757
Acceso en línea:https://hdl.handle.net/11441/182757
https://doi.org/10.1016/j.marenvres.2025.107803
Access Level:acceso abierto
Palabra clave:Marine mussels
Nuclear receptors
Defensome
Xenobiotic metabolism
id ES_7deb6bf816e4e603e45ed42eaa487011
oai_identifier_str oai:idus.us.es:11441/182757
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialisRodrigues, JulianaPlata Calzado, CristinaGómez Román, María PaulaBoo, SergioFonseca, ElzaAraújo, MarioVasconcelos, VitorPrieto Ortega, Ana IsabelJos Gallego, Ángeles MencíaCampos, AlexandreMarine musselsNuclear receptorsDefensomeXenobiotic metabolismFour nr1j1 gene sequences were recently reported in the marine mussel Mytilus galloprovincialis. These genes are orthologs of the nuclear receptor family NR1I found in vertebrates, whose members like the pregnane x receptor (PXR) are known to play a major role in the regulation of xenobiotic metabolism. Building on recent evidence that these four genes encode functional proteins, this study aims to deepen our understanding of nr1j1 genes by analyzing their expression in adult mussels exposed to different diets consisting of microalgae and cyanobacteria, and to the biotoxin okadaic acid. In parallel, we analyzed the expression of several nr1j1 putative target genes, such as cyp3-like and abcb1. Our results showed that gene expression regulation may be organ-specific and timedependent, and that diet and food intake can affect the expression of the genes studied. The highest nr1j1 and cyp3-like transcriptional activation was observed in mussels fed with Tetraselmis sp., while abcb1 genes showed higher expression in mussels fed with toxic cyanobacteria. In contrast, exposure to okadaic acid did not induce changes in the expression of the analyzed genes. This work demonstrates that xenobiotic metabolism responses in mussels can be modulated by diet and exposure to biotoxins, underscoring the importance of diet in the elimination of toxic substances in mussels. The exact mechanisms underlying the observed transcriptional activation of the nr1j1 genes remain unknown.ElsevierNutrición y Bromatología, Toxicología y Medicina LegalEuropean Union (UE)Fundación Portuguesa de Ciencia y Tecnología2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/182757https://doi.org/10.1016/j.marenvres.2025.107803reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMarine Environmental Research, 215, 107803.101086234101119901COMPETE2030-FEDER-00750100https://doi.org/10.1016/j.marenvres.2025.107803info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1827572026-06-17T12:51:07Z
dc.title.none.fl_str_mv Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
title Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
spellingShingle Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
Rodrigues, Juliana
Marine mussels
Nuclear receptors
Defensome
Xenobiotic metabolism
title_short Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
title_full Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
title_fullStr Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
title_full_unstemmed Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
title_sort Effects of okadaic acid and phytoplankton ingestion on nr1j1, cyp, and abcb1 gene expression in Mytilus galloprovincialis
dc.creator.none.fl_str_mv Rodrigues, Juliana
Plata Calzado, Cristina
Gómez Román, María Paula
Boo, Sergio
Fonseca, Elza
Araújo, Mario
Vasconcelos, Vitor
Prieto Ortega, Ana Isabel
Jos Gallego, Ángeles Mencía
Campos, Alexandre
author Rodrigues, Juliana
author_facet Rodrigues, Juliana
Plata Calzado, Cristina
Gómez Román, María Paula
Boo, Sergio
Fonseca, Elza
Araújo, Mario
Vasconcelos, Vitor
Prieto Ortega, Ana Isabel
Jos Gallego, Ángeles Mencía
Campos, Alexandre
author_role author
author2 Plata Calzado, Cristina
Gómez Román, María Paula
Boo, Sergio
Fonseca, Elza
Araújo, Mario
Vasconcelos, Vitor
Prieto Ortega, Ana Isabel
Jos Gallego, Ángeles Mencía
Campos, Alexandre
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Nutrición y Bromatología, Toxicología y Medicina Legal
European Union (UE)
Fundación Portuguesa de Ciencia y Tecnología
dc.subject.none.fl_str_mv Marine mussels
Nuclear receptors
Defensome
Xenobiotic metabolism
topic Marine mussels
Nuclear receptors
Defensome
Xenobiotic metabolism
description Four nr1j1 gene sequences were recently reported in the marine mussel Mytilus galloprovincialis. These genes are orthologs of the nuclear receptor family NR1I found in vertebrates, whose members like the pregnane x receptor (PXR) are known to play a major role in the regulation of xenobiotic metabolism. Building on recent evidence that these four genes encode functional proteins, this study aims to deepen our understanding of nr1j1 genes by analyzing their expression in adult mussels exposed to different diets consisting of microalgae and cyanobacteria, and to the biotoxin okadaic acid. In parallel, we analyzed the expression of several nr1j1 putative target genes, such as cyp3-like and abcb1. Our results showed that gene expression regulation may be organ-specific and timedependent, and that diet and food intake can affect the expression of the genes studied. The highest nr1j1 and cyp3-like transcriptional activation was observed in mussels fed with Tetraselmis sp., while abcb1 genes showed higher expression in mussels fed with toxic cyanobacteria. In contrast, exposure to okadaic acid did not induce changes in the expression of the analyzed genes. This work demonstrates that xenobiotic metabolism responses in mussels can be modulated by diet and exposure to biotoxins, underscoring the importance of diet in the elimination of toxic substances in mussels. The exact mechanisms underlying the observed transcriptional activation of the nr1j1 genes remain unknown.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/182757
https://doi.org/10.1016/j.marenvres.2025.107803
url https://hdl.handle.net/11441/182757
https://doi.org/10.1016/j.marenvres.2025.107803
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Marine Environmental Research, 215, 107803.
101086234
101119901
COMPETE2030-FEDER-00750100
https://doi.org/10.1016/j.marenvres.2025.107803
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411698801115136
score 15,812455

Similares en LA Referencia