Identification of type III secretion inhibitors for plant disease management

Bacterial plant pathogens are among the most devastating threats to agriculture. To date, there are no effective means to control bacterial plant diseases due to the restrictions in the use of antibiotics in agriculture. A novel strategy under study is the use of chemical compounds that inhibit the...

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Detalles Bibliográficos
Autores: Pedro Jové, Roger de, Sebastià, Pau, Valls i Matheu, Marc
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176901
Acceso en línea:https://hdl.handle.net/2445/176901
Access Level:acceso abierto
Palabra clave:Plagues agrícoles
Bacteris patògens
Agricultural pests
Pathogenic bacteria
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spelling Identification of type III secretion inhibitors for plant disease managementPedro Jové, Roger deSebastià, PauValls i Matheu, MarcPlagues agrícolesBacteris patògensAgricultural pestsPathogenic bacteriaBacterial plant pathogens are among the most devastating threats to agriculture. To date, there are no effective means to control bacterial plant diseases due to the restrictions in the use of antibiotics in agriculture. A novel strategy under study is the use of chemical compounds that inhibit the expression of key bacterial virulence determinants. The type III secretion system is essential for virulence of many Gram-negative bacteria because it injects into the plant host cells bacterial proteins that interfere with their immune system. Here, we describe the methodology to identify bacterial type III secretion inhibitors, including a series of protocols that combine in planta and in vitro experiments. We use Ralstonia solanacearum as a model because of the number of genetic tools available in this organism and because it causes bacterial wilt, one of the most threatening plant diseases worldwide. The procedures presented can be used to evaluate the effect of different chemical compounds on bacterial growth and virulence.Springer Science + Business Media2021202120202021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion10 p.application/pdfhttps://hdl.handle.net/2445/176901Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1007/978-1-0716-0954-5_4Methods in Molecular Biology, 2020, vol. 2213, p. 39-48https://doi.org/10.1007/978-1-0716-0954-5_4(c) Springer Science + Business Media, 2020info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1769012026-05-29T05:05:01Z
dc.title.none.fl_str_mv Identification of type III secretion inhibitors for plant disease management
title Identification of type III secretion inhibitors for plant disease management
spellingShingle Identification of type III secretion inhibitors for plant disease management
Pedro Jové, Roger de
Plagues agrícoles
Bacteris patògens
Agricultural pests
Pathogenic bacteria
title_short Identification of type III secretion inhibitors for plant disease management
title_full Identification of type III secretion inhibitors for plant disease management
title_fullStr Identification of type III secretion inhibitors for plant disease management
title_full_unstemmed Identification of type III secretion inhibitors for plant disease management
title_sort Identification of type III secretion inhibitors for plant disease management
dc.creator.none.fl_str_mv Pedro Jové, Roger de
Sebastià, Pau
Valls i Matheu, Marc
author Pedro Jové, Roger de
author_facet Pedro Jové, Roger de
Sebastià, Pau
Valls i Matheu, Marc
author_role author
author2 Sebastià, Pau
Valls i Matheu, Marc
author2_role author
author
dc.subject.none.fl_str_mv Plagues agrícoles
Bacteris patògens
Agricultural pests
Pathogenic bacteria
topic Plagues agrícoles
Bacteris patògens
Agricultural pests
Pathogenic bacteria
description Bacterial plant pathogens are among the most devastating threats to agriculture. To date, there are no effective means to control bacterial plant diseases due to the restrictions in the use of antibiotics in agriculture. A novel strategy under study is the use of chemical compounds that inhibit the expression of key bacterial virulence determinants. The type III secretion system is essential for virulence of many Gram-negative bacteria because it injects into the plant host cells bacterial proteins that interfere with their immune system. Here, we describe the methodology to identify bacterial type III secretion inhibitors, including a series of protocols that combine in planta and in vitro experiments. We use Ralstonia solanacearum as a model because of the number of genetic tools available in this organism and because it causes bacterial wilt, one of the most threatening plant diseases worldwide. The procedures presented can be used to evaluate the effect of different chemical compounds on bacterial growth and virulence.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176901
url https://hdl.handle.net/2445/176901
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1007/978-1-0716-0954-5_4
Methods in Molecular Biology, 2020, vol. 2213, p. 39-48
https://doi.org/10.1007/978-1-0716-0954-5_4
dc.rights.none.fl_str_mv (c) Springer Science + Business Media, 2020
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Springer Science + Business Media, 2020
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Springer Science + Business Media
publisher.none.fl_str_mv Springer Science + Business Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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