High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods

Santiago Cuellar, Paula; Jiménez Belenguer, Ana Isabel|||0000-0002-3329-7221; García Hernández, Jorge|||0000-0003-1258-6128; Montes-Estellés, Rosa M|||0000-0002-4599-6644; Hernández Pérez, Manuel|||0000-0003-1986-6501; Castillo López, María-Ángeles|||0000-0002-8534-8940; Ferrús Pérez, Mª Antonia; Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157

High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods

[EN] Salmonella spp. is one of the most important causal agents of food-borne illness in developed countries and its presence in irrigation water poses a risk to public health. Its detection in environmental samples is not easy when culture methods are used, and molecular techniques such as PCR or r...

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Autores:	Santiago Cuellar, Paula, Jiménez Belenguer, Ana Isabel\|\|\|0000-0002-3329-7221, García Hernández, Jorge\|\|\|0000-0003-1258-6128, Montes-Estellés, Rosa M\|\|\|0000-0002-4599-6644, Hernández Pérez, Manuel\|\|\|0000-0003-1986-6501, Castillo López, María-Ángeles\|\|\|0000-0002-8534-8940, Ferrús Pérez, Mª Antonia, Moreno Trigos, Mª Yolanda\|\|\|0000-0003-3688-5157
Tipo de recurso:	artículo
Fecha de publicación:	2018
País:	España
Institución:	Universitat Politècnica de València (UPV)
Repositorio:	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:	inglés
OAI Identifier:	oai:riunet.upv.es:10251/117578
Acceso en línea:	https://riunet.upv.es/handle/10251/117578
Access Level:	acceso abierto
Palabra clave:	FISH Molecular detection Reclaimed water Salmonella Wastewater MICROBIOLOGIA

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spelling	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methodsSantiago Cuellar, PaulaJiménez Belenguer, Ana Isabel\|\|\|0000-0002-3329-7221García Hernández, Jorge\|\|\|0000-0003-1258-6128Montes-Estellés, Rosa M\|\|\|0000-0002-4599-6644Hernández Pérez, Manuel\|\|\|0000-0003-1986-6501Castillo López, María-Ángeles\|\|\|0000-0002-8534-8940Ferrús Pérez, Mª AntoniaMoreno Trigos, Mª Yolanda\|\|\|0000-0003-3688-5157FISHMolecular detectionReclaimed waterSalmonellaWastewaterMICROBIOLOGIA[EN] Salmonella spp. is one of the most important causal agents of food-borne illness in developed countries and its presence in irrigation water poses a risk to public health. Its detection in environmental samples is not easy when culture methods are used, and molecular techniques such as PCR or ribosomal rRNA probe hybridization (Fluorescent zn situ Hybridization, FISH) are outstanding alternatives. The aim of this work was to determine the environmental risk due to the presence of Salmonella spp. in wastewater by culture, PCR and FISH. A new specific rDNA probe for Salmonella was designed and its efficiency was compared with the rest of methods Serotype and antibiotic resistance of isolated strains were determined. Forty-five wastewater samples (collected from two secondary wastewater treatment plants) were analysed. Salmonella strains were isolated in 24 wastewater samples (53%), two of them after disinfection treatment. Twenty-three Salmonella strains exhibited resistance to one or more antimicrobial agent. Analysis of wastewater samples yielded PCR positive results for Salmonella in 28 out of the 45 wastewater samples (62%). FISH analysis allowed for the detection of Salmonella in 27 (60%) samples. By using molecular methods, Salmonella was detected in four samples after disinfection treatment. These results show the prevalence of Salmonella in reclaimed wastewater even after U.V. disinfection, what is a matter of public health concern, the high rates of resistance to antibiotics and the adequacy of molecular methods for its rapid detection. FISH method, with SA23 probe developed and assayed in this work provides a tool for detecting Salmonella in water within few hours, with a high rate of effectiveness.Authors gratefully acknowledge the financial support from the Ministerio de Ciencia e Innovacion (Project: AGL2008-05275-C03-02).ElsevierDepartamento de BiotecnologíaInstituto Universitario de Ingeniería del Agua y del Medio AmbienteCentro Avanzado de Microbiología AplicadaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/117578reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2008-05275-C03-02 ANALISIS INTEGRADO DE RIESGOS EN AGUA Y ALIMENTOS PARA BACTERIAS PATOGENAS EMERGENTES POR METODOS MOLECULARES: DESINFECCION, SUPERVIVENCIA Y TRAZABILIDADopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1175782026-06-13T07:49:27Z
dc.title.none.fl_str_mv	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
title	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
spellingShingle	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods Santiago Cuellar, Paula FISH Molecular detection Reclaimed water Salmonella Wastewater MICROBIOLOGIA
title_short	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
title_full	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
title_fullStr	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
title_full_unstemmed	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
title_sort	High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods
dc.creator.none.fl_str_mv	Santiago Cuellar, Paula Jiménez Belenguer, Ana Isabel\|\|\|0000-0002-3329-7221 García Hernández, Jorge\|\|\|0000-0003-1258-6128 Montes-Estellés, Rosa M\|\|\|0000-0002-4599-6644 Hernández Pérez, Manuel\|\|\|0000-0003-1986-6501 Castillo López, María-Ángeles\|\|\|0000-0002-8534-8940 Ferrús Pérez, Mª Antonia Moreno Trigos, Mª Yolanda\|\|\|0000-0003-3688-5157
author	Santiago Cuellar, Paula
author_facet	Santiago Cuellar, Paula Jiménez Belenguer, Ana Isabel\|\|\|0000-0002-3329-7221 García Hernández, Jorge\|\|\|0000-0003-1258-6128 Montes-Estellés, Rosa M\|\|\|0000-0002-4599-6644 Hernández Pérez, Manuel\|\|\|0000-0003-1986-6501 Castillo López, María-Ángeles\|\|\|0000-0002-8534-8940 Ferrús Pérez, Mª Antonia Moreno Trigos, Mª Yolanda\|\|\|0000-0003-3688-5157
author_role	author
author2	Jiménez Belenguer, Ana Isabel\|\|\|0000-0002-3329-7221 García Hernández, Jorge\|\|\|0000-0003-1258-6128 Montes-Estellés, Rosa M\|\|\|0000-0002-4599-6644 Hernández Pérez, Manuel\|\|\|0000-0003-1986-6501 Castillo López, María-Ángeles\|\|\|0000-0002-8534-8940 Ferrús Pérez, Mª Antonia Moreno Trigos, Mª Yolanda\|\|\|0000-0003-3688-5157
author2_role	author author author author author author author
dc.contributor.none.fl_str_mv	Departamento de Biotecnología Instituto Universitario de Ingeniería del Agua y del Medio Ambiente Centro Avanzado de Microbiología Aplicada Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural Ministerio de Ciencia e Innovación Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv	FISH Molecular detection Reclaimed water Salmonella Wastewater MICROBIOLOGIA
topic	FISH Molecular detection Reclaimed water Salmonella Wastewater MICROBIOLOGIA
description	[EN] Salmonella spp. is one of the most important causal agents of food-borne illness in developed countries and its presence in irrigation water poses a risk to public health. Its detection in environmental samples is not easy when culture methods are used, and molecular techniques such as PCR or ribosomal rRNA probe hybridization (Fluorescent zn situ Hybridization, FISH) are outstanding alternatives. The aim of this work was to determine the environmental risk due to the presence of Salmonella spp. in wastewater by culture, PCR and FISH. A new specific rDNA probe for Salmonella was designed and its efficiency was compared with the rest of methods Serotype and antibiotic resistance of isolated strains were determined. Forty-five wastewater samples (collected from two secondary wastewater treatment plants) were analysed. Salmonella strains were isolated in 24 wastewater samples (53%), two of them after disinfection treatment. Twenty-three Salmonella strains exhibited resistance to one or more antimicrobial agent. Analysis of wastewater samples yielded PCR positive results for Salmonella in 28 out of the 45 wastewater samples (62%). FISH analysis allowed for the detection of Salmonella in 27 (60%) samples. By using molecular methods, Salmonella was detected in four samples after disinfection treatment. These results show the prevalence of Salmonella in reclaimed wastewater even after U.V. disinfection, what is a matter of public health concern, the high rates of resistance to antibiotics and the adequacy of molecular methods for its rapid detection. FISH method, with SA23 probe developed and assayed in this work provides a tool for detecting Salmonella in water within few hours, with a high rate of effectiveness.
publishDate	2018
dc.date.none.fl_str_mv	2018 2018-01-01
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://riunet.upv.es/handle/10251/117578
url	https://riunet.upv.es/handle/10251/117578
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2008-05275-C03-02 ANALISIS INTEGRADO DE RIESGOS EN AGUA Y ALIMENTOS PARA BACTERIAS PATOGENAS EMERGENTES POR METODOS MOLECULARES: DESINFECCION, SUPERVIVENCIA Y TRAZABILIDAD
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV)
instname_str	Universitat Politècnica de València (UPV)
reponame_str	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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High prevalence of Salmonella spp. in wastewater reused for irrigation assessed by molecular methods

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