Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis

Glycogen is a glucose-storage polysaccharide molecule present in animals, fungi and bacteria. The enzyme glycogenin can self-glycosylate, forming an oligosaccharide chain that primes glycogen synthesis. This priming role of glycogenin was first believed to be essential for glycogen synthesis, but gl...

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Authors: Tan, Xinle, Testoni, Giorgia, Sullivan, Mitchell A., López Soldado, Iliana, Vilaplana, Francisco, Gilbert, Robert G., Guinovart, Joan J., Schulz, Benjamin L., Castells Duran, Jordi
Format: article
Publication Date:2025
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/4870
Online Access:http://hdl.handle.net/20.500.14342/4870
https://doi.org/10.1016/j.ijbiomac.2024.139084
Access Level:Open access
Keyword:Glycogen
Glycogenin
Glucose metabolism
Glycogen α particles
Liver
Glicogen
Glucosa--Metabolisme
Fetge
547
577
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oai_identifier_str oai:recercat.cat:20.500.14342/4870
network_acronym_str ES
network_name_str España
repository_id_str
spelling Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasisTan, XinleTestoni, GiorgiaSullivan, Mitchell A.López Soldado, IlianaVilaplana, FranciscoGilbert, Robert G.Guinovart, Joan J.Schulz, Benjamin L.Castells Duran, JordiGlycogenGlycogeninGlucose metabolismGlycogen α particlesLiverGlicogenGlucosa--MetabolismeFetge547577Glycogen is a glucose-storage polysaccharide molecule present in animals, fungi and bacteria. The enzyme glycogenin can self-glycosylate, forming an oligosaccharide chain that primes glycogen synthesis. This priming role of glycogenin was first believed to be essential for glycogen synthesis, but glycogen was then found in the skeletal muscle, heart, liver and brain of glycogenin-knockout mice (Gyg KO), thereby showing that glycogen can be synthesized without glycogenin. Within the liver, glycogen is present in the form of individual glycogen particles, called β particles, and larger composite aggregates of linked β particles, called α particles. Previous studies suggested that liver glycogenin plays a role in linking β particles into α particles and thus participating in glucose homeostasis, which implies that α particles would be absent in Gyg KO mice liver. Here we test this through targeted characterization of glycogen structure and through proteomic and metabolic studies on Gyg KO mice. The results show that, contrary to what had been believed, glycogenin is not necessary for normal liver-glycogen metabolism.info:eu-repo/semantics/publishedVersionElsevierUniversitat Ramon Llull. IQS2025info:eu-repo/semantics/articlep.13http://hdl.handle.net/20.500.14342/4870https://doi.org/10.1016/j.ijbiomac.2024.139084reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInternational Journal of Biological Macromolecules 2025, 291, 139084info:eu-repo/grantAgreement/MCI/PN I+D/PID2020-118699GB-I00© L'autor/aAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/48702026-05-29T05:05:01Z
dc.title.none.fl_str_mv Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
title Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
spellingShingle Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
Tan, Xinle
Glycogen
Glycogenin
Glucose metabolism
Glycogen α particles
Liver
Glicogen
Glucosa--Metabolisme
Fetge
547
577
title_short Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
title_full Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
title_fullStr Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
title_full_unstemmed Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
title_sort Glycogenin is dispensable for normal liver glycogen metabolism and body glucose homeostasis
dc.creator.none.fl_str_mv Tan, Xinle
Testoni, Giorgia
Sullivan, Mitchell A.
López Soldado, Iliana
Vilaplana, Francisco
Gilbert, Robert G.
Guinovart, Joan J.
Schulz, Benjamin L.
Castells Duran, Jordi
author Tan, Xinle
author_facet Tan, Xinle
Testoni, Giorgia
Sullivan, Mitchell A.
López Soldado, Iliana
Vilaplana, Francisco
Gilbert, Robert G.
Guinovart, Joan J.
Schulz, Benjamin L.
Castells Duran, Jordi
author_role author
author2 Testoni, Giorgia
Sullivan, Mitchell A.
López Soldado, Iliana
Vilaplana, Francisco
Gilbert, Robert G.
Guinovart, Joan J.
Schulz, Benjamin L.
Castells Duran, Jordi
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Glycogen
Glycogenin
Glucose metabolism
Glycogen α particles
Liver
Glicogen
Glucosa--Metabolisme
Fetge
547
577
topic Glycogen
Glycogenin
Glucose metabolism
Glycogen α particles
Liver
Glicogen
Glucosa--Metabolisme
Fetge
547
577
description Glycogen is a glucose-storage polysaccharide molecule present in animals, fungi and bacteria. The enzyme glycogenin can self-glycosylate, forming an oligosaccharide chain that primes glycogen synthesis. This priming role of glycogenin was first believed to be essential for glycogen synthesis, but glycogen was then found in the skeletal muscle, heart, liver and brain of glycogenin-knockout mice (Gyg KO), thereby showing that glycogen can be synthesized without glycogenin. Within the liver, glycogen is present in the form of individual glycogen particles, called β particles, and larger composite aggregates of linked β particles, called α particles. Previous studies suggested that liver glycogenin plays a role in linking β particles into α particles and thus participating in glucose homeostasis, which implies that α particles would be absent in Gyg KO mice liver. Here we test this through targeted characterization of glycogen structure and through proteomic and metabolic studies on Gyg KO mice. The results show that, contrary to what had been believed, glycogenin is not necessary for normal liver-glycogen metabolism.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/4870
https://doi.org/10.1016/j.ijbiomac.2024.139084
url http://hdl.handle.net/20.500.14342/4870
https://doi.org/10.1016/j.ijbiomac.2024.139084
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Biological Macromolecules 2025, 291, 139084
info:eu-repo/grantAgreement/MCI/PN I+D/PID2020-118699GB-I00
dc.rights.none.fl_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv p.13
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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