Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes

The effect of the PPh3 group in the antitumor activity of some new organometallic Ruthenium (II) complexes has been investigated. Several complexes of the type [Ru(II)(Cl)(PPh3)(Lig-N)], [Ru(II)(Cl)2(Lig-N)] (where Lig-N=pyridine derivate) and [Ru(II)(Cl)(PPh3)2], have been synthesized and character...

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Autores: Saez-Vigo, Ruben, Lorenzo, Julia, Prieto Villanueva, Ma. José, Font Bardia, Ma. Mercedes, Calvet Pallàs, Maria Teresa, Omeñaca, N., Vilaseca, M., Moreno Martínez, Virtudes
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/165285
Acceso en línea:https://hdl.handle.net/2445/165285
Access Level:acceso abierto
Palabra clave:Ruteni
Compostos organometàl·lics
Ruthenium
Organometallic compounds
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spelling Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexesSaez-Vigo, RubenLorenzo, JuliaPrieto Villanueva, Ma. JoséFont Bardia, Ma. MercedesCalvet Pallàs, Maria TeresaOmeñaca, N.Vilaseca, M.Moreno Martínez, VirtudesRuteniCompostos organometàl·licsRutheniumOrganometallic compoundsThe effect of the PPh3 group in the antitumor activity of some new organometallic Ruthenium (II) complexes has been investigated. Several complexes of the type [Ru(II)(Cl)(PPh3)(Lig-N)], [Ru(II)(Cl)2(Lig-N)] (where Lig-N=pyridine derivate) and [Ru(II)(Cl)(PPh3)2], have been synthesized and characterized, and an important increment of the antitumor activity and cytotoxicity of the complexes due to the presence of PPh3 moiety has been demonstrated, affording IC50 values of 5.2 μM in HL-60 tumour cell lines. Atomic Force Microscopy, Circular Dichroism and Electrophoresis experiments have proved that these complexes can bind DNA resulting in a distortion of both secondary and tertiary structures. Ethidium bromide displacement Fluorescence Spectroscopy studies and Viscosity measurements support that the presence of PPh3 group induces intercalation interactions with DNA. Indeed, crystallographic analysis, suggest that intra-molecular π-π interactions could be involved in the intercalation within DNA base pairs. Furthermore, HPLC-MS studies have confirmed a strong interaction between Ruthenium complexes and proteins (Ubiquitin and Potato Carboxypeptidase Inhibitor -PCI-) including slower kinetic due to the presence of PPh3 moiety, which could have an important role in detoxification mechanism and others. Finally, Ion Mobility Mass Spectrometry (IMMS) experiments have proved that there is no change in the structural conformation of the proteins owing to their bonding to Ruthenium complexes. This seems particularly important in the case of PCI, that may be a suitable candidate for vehiculizing these complexes in a selective manner into tumour cells. In agreement with these results, further investigations should be carried out to clarify either there is a favoured binding to DNA or to specific proteins, thus to elucidate their main biological target.Elsevier B.V.2020202020142020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion38 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/165285Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.jinorgbio.2014.03.002Journal of Inorganic Biochemistry, 2014, vol. 136, p. 1-12https://doi.org/10.1016/j.jinorgbio.2014.03.002(c) Elsevier B.V., 2014info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1652852026-05-29T05:05:01Z
dc.title.none.fl_str_mv Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
title Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
spellingShingle Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
Saez-Vigo, Ruben
Ruteni
Compostos organometàl·lics
Ruthenium
Organometallic compounds
title_short Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
title_full Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
title_fullStr Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
title_full_unstemmed Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
title_sort Influence of PPh3 moiety in the anticancer activity of new organometallic ruthenium complexes
dc.creator.none.fl_str_mv Saez-Vigo, Ruben
Lorenzo, Julia
Prieto Villanueva, Ma. José
Font Bardia, Ma. Mercedes
Calvet Pallàs, Maria Teresa
Omeñaca, N.
Vilaseca, M.
Moreno Martínez, Virtudes
author Saez-Vigo, Ruben
author_facet Saez-Vigo, Ruben
Lorenzo, Julia
Prieto Villanueva, Ma. José
Font Bardia, Ma. Mercedes
Calvet Pallàs, Maria Teresa
Omeñaca, N.
Vilaseca, M.
Moreno Martínez, Virtudes
author_role author
author2 Lorenzo, Julia
Prieto Villanueva, Ma. José
Font Bardia, Ma. Mercedes
Calvet Pallàs, Maria Teresa
Omeñaca, N.
Vilaseca, M.
Moreno Martínez, Virtudes
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ruteni
Compostos organometàl·lics
Ruthenium
Organometallic compounds
topic Ruteni
Compostos organometàl·lics
Ruthenium
Organometallic compounds
description The effect of the PPh3 group in the antitumor activity of some new organometallic Ruthenium (II) complexes has been investigated. Several complexes of the type [Ru(II)(Cl)(PPh3)(Lig-N)], [Ru(II)(Cl)2(Lig-N)] (where Lig-N=pyridine derivate) and [Ru(II)(Cl)(PPh3)2], have been synthesized and characterized, and an important increment of the antitumor activity and cytotoxicity of the complexes due to the presence of PPh3 moiety has been demonstrated, affording IC50 values of 5.2 μM in HL-60 tumour cell lines. Atomic Force Microscopy, Circular Dichroism and Electrophoresis experiments have proved that these complexes can bind DNA resulting in a distortion of both secondary and tertiary structures. Ethidium bromide displacement Fluorescence Spectroscopy studies and Viscosity measurements support that the presence of PPh3 group induces intercalation interactions with DNA. Indeed, crystallographic analysis, suggest that intra-molecular π-π interactions could be involved in the intercalation within DNA base pairs. Furthermore, HPLC-MS studies have confirmed a strong interaction between Ruthenium complexes and proteins (Ubiquitin and Potato Carboxypeptidase Inhibitor -PCI-) including slower kinetic due to the presence of PPh3 moiety, which could have an important role in detoxification mechanism and others. Finally, Ion Mobility Mass Spectrometry (IMMS) experiments have proved that there is no change in the structural conformation of the proteins owing to their bonding to Ruthenium complexes. This seems particularly important in the case of PCI, that may be a suitable candidate for vehiculizing these complexes in a selective manner into tumour cells. In agreement with these results, further investigations should be carried out to clarify either there is a favoured binding to DNA or to specific proteins, thus to elucidate their main biological target.
publishDate 2014
dc.date.none.fl_str_mv 2014
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/165285
url https://hdl.handle.net/2445/165285
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.jinorgbio.2014.03.002
Journal of Inorganic Biochemistry, 2014, vol. 136, p. 1-12
https://doi.org/10.1016/j.jinorgbio.2014.03.002
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2014
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2014
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 38 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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