Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing
This work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytic...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/105630 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/105630 |
| Access Level: | acceso abierto |
| Palabra clave: | 543 Electrochemical bioplatform Endostatin Angiogenesis Colorectal cancer aggressiveness Tissue Plasma Ciencias 2301 Química Analítica |
| id |
ES_7d8b34a2984c19d7907c48d23c4911d7 |
|---|---|
| oai_identifier_str |
oai:docta.ucm.es:20.500.14352/105630 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensingTejerina Miranda, SandraPedrero Muñoz, MaríaBlázquez García, MarinaSerafín González-Carrato, VerónicaMontero Calle, AnaGarranzo Asensio, MaríaReviejo García, Ángel JulioPingarrón Carrazón, José ManuelBarderas Manchado, RodrigoCampuzano Ruiz, Susana543Electrochemical bioplatformEndostatinAngiogenesisColorectal cancer aggressivenessTissuePlasmaCiencias2301 Química AnalíticaThis work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL−1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies.ElsevierUniversidad Complutense de Madrid20242024-01-0120242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/105630reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1056302026-06-02T12:44:21Z |
| dc.title.none.fl_str_mv |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| title |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| spellingShingle |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing Tejerina Miranda, Sandra 543 Electrochemical bioplatform Endostatin Angiogenesis Colorectal cancer aggressiveness Tissue Plasma Ciencias 2301 Química Analítica |
| title_short |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| title_full |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| title_fullStr |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| title_full_unstemmed |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| title_sort |
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing |
| dc.creator.none.fl_str_mv |
Tejerina Miranda, Sandra Pedrero Muñoz, María Blázquez García, Marina Serafín González-Carrato, Verónica Montero Calle, Ana Garranzo Asensio, María Reviejo García, Ángel Julio Pingarrón Carrazón, José Manuel Barderas Manchado, Rodrigo Campuzano Ruiz, Susana |
| author |
Tejerina Miranda, Sandra |
| author_facet |
Tejerina Miranda, Sandra Pedrero Muñoz, María Blázquez García, Marina Serafín González-Carrato, Verónica Montero Calle, Ana Garranzo Asensio, María Reviejo García, Ángel Julio Pingarrón Carrazón, José Manuel Barderas Manchado, Rodrigo Campuzano Ruiz, Susana |
| author_role |
author |
| author2 |
Pedrero Muñoz, María Blázquez García, Marina Serafín González-Carrato, Verónica Montero Calle, Ana Garranzo Asensio, María Reviejo García, Ángel Julio Pingarrón Carrazón, José Manuel Barderas Manchado, Rodrigo Campuzano Ruiz, Susana |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad Complutense de Madrid |
| dc.subject.none.fl_str_mv |
543 Electrochemical bioplatform Endostatin Angiogenesis Colorectal cancer aggressiveness Tissue Plasma Ciencias 2301 Química Analítica |
| topic |
543 Electrochemical bioplatform Endostatin Angiogenesis Colorectal cancer aggressiveness Tissue Plasma Ciencias 2301 Química Analítica |
| description |
This work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL−1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024-01-01 2024 2024-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.14352/105630 |
| url |
https://hdl.handle.net/20.500.14352/105630 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM) |
| instname_str |
Universidad Complutense de Madrid (UCM) |
| reponame_str |
Docta Complutense |
| collection |
Docta Complutense |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869411671917723648 |
| score |
15,811543 |