Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae

In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular con...

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Autores: Ramos Sáenz, Ana, González Álvarez, Daniel, Rodríguez Galán, Olga, Rodríguez Gil, Alfonso, Gaspar, Sonia G., Villalobo Polo, Eduardo, Dosil, Mercedes, Cruz Díaz, Jesús de la
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/143475
Acesso em linha:https://hdl.handle.net/11441/143475
https://doi.org/10.1261/rna.072116.119
Access Level:Acceso aberto
Palavra-chave:ribosome biogenesis
pre-rRNA processing
MYBBP1A
ribosomal proteins uL23 and eL27
nucleolus
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repository_id_str
spelling Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiaeRamos Sáenz, AnaGonzález Álvarez, DanielRodríguez Galán, OlgaRodríguez Gil, AlfonsoGaspar, Sonia G.Villalobo Polo, EduardoDosil, MercedesCruz Díaz, Jesús de laribosome biogenesispre-rRNA processingMYBBP1Aribosomal proteins uL23 and eL27nucleolusIn Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5-depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis.Cold Spring Harbor Laboratory PressGenéticaMicrobiologíaMinisterio de Economía y Competitividad (MINECO). EspañaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)Agencia Estatal de Investigación. España2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/143475https://doi.org/10.1261/rna.072116.119reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésRNA, 25 (11), 1561-1575.BFU2016-75352-PBFU2014-52729-PBFU2017-88192-Phttps://dx.doi.org/10.1261/rna.072116.119info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1434752026-06-17T12:51:07Z
dc.title.none.fl_str_mv Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
title Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
spellingShingle Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
Ramos Sáenz, Ana
ribosome biogenesis
pre-rRNA processing
MYBBP1A
ribosomal proteins uL23 and eL27
nucleolus
title_short Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
title_full Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
title_fullStr Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
title_full_unstemmed Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
title_sort Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae
dc.creator.none.fl_str_mv Ramos Sáenz, Ana
González Álvarez, Daniel
Rodríguez Galán, Olga
Rodríguez Gil, Alfonso
Gaspar, Sonia G.
Villalobo Polo, Eduardo
Dosil, Mercedes
Cruz Díaz, Jesús de la
author Ramos Sáenz, Ana
author_facet Ramos Sáenz, Ana
González Álvarez, Daniel
Rodríguez Galán, Olga
Rodríguez Gil, Alfonso
Gaspar, Sonia G.
Villalobo Polo, Eduardo
Dosil, Mercedes
Cruz Díaz, Jesús de la
author_role author
author2 González Álvarez, Daniel
Rodríguez Galán, Olga
Rodríguez Gil, Alfonso
Gaspar, Sonia G.
Villalobo Polo, Eduardo
Dosil, Mercedes
Cruz Díaz, Jesús de la
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
Microbiología
Ministerio de Economía y Competitividad (MINECO). España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Agencia Estatal de Investigación. España
dc.subject.none.fl_str_mv ribosome biogenesis
pre-rRNA processing
MYBBP1A
ribosomal proteins uL23 and eL27
nucleolus
topic ribosome biogenesis
pre-rRNA processing
MYBBP1A
ribosomal proteins uL23 and eL27
nucleolus
description In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5-depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/143475
https://doi.org/10.1261/rna.072116.119
url https://hdl.handle.net/11441/143475
https://doi.org/10.1261/rna.072116.119
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv RNA, 25 (11), 1561-1575.
BFU2016-75352-P
BFU2014-52729-P
BFU2017-88192-P
https://dx.doi.org/10.1261/rna.072116.119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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