Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).

Most Calligonum species are desert plants, characteristic of the Saharan bioclimatic region. All species karyologically analyzed until present have the basic chromosome number x = 9 and comprise diploids, triploids and tetraploids. The Tunisian flora comprises diploid Calligonum arich and C. azel, o...

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Detalles Bibliográficos
Autores: Gouja, H., Garnatje i Roca, Teresa, Hidalgo Grani, Oriane, Neffati, M., Raies, A., Garcia Giménez, Sònia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/127985
Acceso en línea:https://hdl.handle.net/2445/127985
Access Level:acceso abierto
Palabra clave:Plantes silvestres
Citogenètica
Ribosomes
Wild flowers
Cytogenetics
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spelling Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).Gouja, H.Garnatje i Roca, TeresaHidalgo Grani, OrianeNeffati, M.Raies, A.Garcia Giménez, SòniaPlantes silvestresCitogenèticaRibosomesWild flowersCytogeneticsRibosomesMost Calligonum species are desert plants, characteristic of the Saharan bioclimatic region. All species karyologically analyzed until present have the basic chromosome number x = 9 and comprise diploids, triploids and tetraploids. The Tunisian flora comprises diploid Calligonum arich and C. azel, of restricted distribution, and the tetraploid C. comosum with wider distribution. Analyses of their karyotypes and polyploidisation-linked rDNA changes by orcein staining, fluorochrome banding with chromomycin A3 and fluorescent in situ hybridisation with 5S and 26S ribosomal DNA probes have been performed. We report the chromosome number for Calligonum arich (2n = 18) as well as the diploid level for C. comosum for the first time. Chromosome counts have also verified the earlier described tetraploid cytotype (2n = 36) of C. comosum. A general pattern of six GC-rich bands as well as two 35S sites and four 5S sites is described for Calligonum species at the diploid level although there is intraspecific variation regarding the site number in a second type of C. comosum, with one pair of 35S rDNA sites and two pairs of 5S rDNA sites. The tetraploid cytotype of C. comosum has undergone locus loss and genome downsizing. Genome size assessments confirmed previous data. Nonetheless, statistically significant differences were found depending on the type of tissue used for estimation. Measurements from seeds had always larger values than from leaves. The presence of cytosolic compounds in leaves, interfering with DNA staining, is discussed as a possible cause of the differences.Springer Verlag2019201920152019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/127985Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1007/s00606-014-1183-9Plant Systematics and Evolution, 2015, vol. 301, num. 6, p. 1569-1579https://doi.org/10.1007/s00606-014-1183-9(c) Springer Verlag, 2015info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1279852026-05-29T05:05:01Z
dc.title.none.fl_str_mv Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
title Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
spellingShingle Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
Gouja, H.
Plantes silvestres
Citogenètica
Ribosomes
Wild flowers
Cytogenetics
Ribosomes
title_short Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
title_full Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
title_fullStr Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
title_full_unstemmed Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
title_sort Physical mapping of ribosomal DNA and genome size in diploid and polyploid North African Calligonum species (Polygonaceae).
dc.creator.none.fl_str_mv Gouja, H.
Garnatje i Roca, Teresa
Hidalgo Grani, Oriane
Neffati, M.
Raies, A.
Garcia Giménez, Sònia
author Gouja, H.
author_facet Gouja, H.
Garnatje i Roca, Teresa
Hidalgo Grani, Oriane
Neffati, M.
Raies, A.
Garcia Giménez, Sònia
author_role author
author2 Garnatje i Roca, Teresa
Hidalgo Grani, Oriane
Neffati, M.
Raies, A.
Garcia Giménez, Sònia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Plantes silvestres
Citogenètica
Ribosomes
Wild flowers
Cytogenetics
Ribosomes
topic Plantes silvestres
Citogenètica
Ribosomes
Wild flowers
Cytogenetics
Ribosomes
description Most Calligonum species are desert plants, characteristic of the Saharan bioclimatic region. All species karyologically analyzed until present have the basic chromosome number x = 9 and comprise diploids, triploids and tetraploids. The Tunisian flora comprises diploid Calligonum arich and C. azel, of restricted distribution, and the tetraploid C. comosum with wider distribution. Analyses of their karyotypes and polyploidisation-linked rDNA changes by orcein staining, fluorochrome banding with chromomycin A3 and fluorescent in situ hybridisation with 5S and 26S ribosomal DNA probes have been performed. We report the chromosome number for Calligonum arich (2n = 18) as well as the diploid level for C. comosum for the first time. Chromosome counts have also verified the earlier described tetraploid cytotype (2n = 36) of C. comosum. A general pattern of six GC-rich bands as well as two 35S sites and four 5S sites is described for Calligonum species at the diploid level although there is intraspecific variation regarding the site number in a second type of C. comosum, with one pair of 35S rDNA sites and two pairs of 5S rDNA sites. The tetraploid cytotype of C. comosum has undergone locus loss and genome downsizing. Genome size assessments confirmed previous data. Nonetheless, statistically significant differences were found depending on the type of tissue used for estimation. Measurements from seeds had always larger values than from leaves. The presence of cytosolic compounds in leaves, interfering with DNA staining, is discussed as a possible cause of the differences.
publishDate 2015
dc.date.none.fl_str_mv 2015
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/127985
url https://hdl.handle.net/2445/127985
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1007/s00606-014-1183-9
Plant Systematics and Evolution, 2015, vol. 301, num. 6, p. 1569-1579
https://doi.org/10.1007/s00606-014-1183-9
dc.rights.none.fl_str_mv (c) Springer Verlag, 2015
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Springer Verlag, 2015
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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