Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro

Micro/nanoplastics (MNPLs) are environmental contaminants originating mainly from plastic waste degradation that pose potential health risks. Inhalation is a major exposure route, as evidenced by their detection in human lungs, with polyethylene terephthalate (PET) among the most abundant particles...

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Autores: Gutierrez Garcia, Javier|||0009-0007-7376-8933, Egea, Raquel|||0000-0002-6201-4112, Barguilla, Irene|||0000-0003-1081-4457, Nymark, Penny|||0000-0002-3435-7775, García Rodríguez, Alba|||0000-0002-1175-7418, Guyot, Boris|||0000-0002-4512-7123, Maguer-Satta, Veronique|||0000-0002-1556-068X, Marcos Dauder, Ricardo|||0000-0001-7891-357X, Rubio Lorente, Laura|||0000-0002-2088-3803, Hernández Bonilla, Alba|||0000-0001-6938-1233
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:313097
Acceso en línea:https://ddd.uab.cat/record/313097
https://dx.doi.org/urn:doi:10.1021/acs.est.5c01628
Access Level:acceso abierto
Palabra clave:Nanoplastics (NPLs)
Polyethylene terephthalate (PET)
Carcinogenicity
Respiratory toxicity
Chronic exposure
New approach methodologies (NAMs)
Human health risk
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spelling Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In VitroGutierrez Garcia, Javier|||0009-0007-7376-8933Egea, Raquel|||0000-0002-6201-4112Barguilla, Irene|||0000-0003-1081-4457Nymark, Penny|||0000-0002-3435-7775García Rodríguez, Alba|||0000-0002-1175-7418Guyot, Boris|||0000-0002-4512-7123Maguer-Satta, Veronique|||0000-0002-1556-068XMarcos Dauder, Ricardo|||0000-0001-7891-357XRubio Lorente, Laura|||0000-0002-2088-3803Hernández Bonilla, Alba|||0000-0001-6938-1233Nanoplastics (NPLs)Polyethylene terephthalate (PET)CarcinogenicityRespiratory toxicityChronic exposureNew approach methodologies (NAMs)Human health riskMicro/nanoplastics (MNPLs) are environmental contaminants originating mainly from plastic waste degradation that pose potential health risks. Inhalation is a major exposure route, as evidenced by their detection in human lungs, with polyethylene terephthalate (PET) among the most abundant particles in respiratory airways. However, the harmful effects of particle bioaccumulation remain unclear, as chronic effects are understudied. To assess long-term effects, specifically carcinogenic effects, BEAS-2B cells were exposed to PET-NPLs for 30 weeks. Genotoxicity, carcinogenic phenotypic hallmarks, and a panel of genes and pathways associated with cell transformation and lung cancer were examined and compared across three exposure durations. No significant effects were observed after 24 h or 15 weeks of exposure. However, a 30-week exposure led to increased genotoxic damage, anchorage-independent growth, and invasive potential. Transcriptomic analysis showed the upregulation of several oncogenes and lung cancer-associated genes at the end of the exposure. Further analysis revealed an increase in differentially expressed genes over time and a temporal gradient of lung cancer-related genes. Altogether, the data suggest PET-NPLs' potential carcinogenicity after extended exposure, highlighting serious long-term health risks of MNPLs. Assessing their carcinogenic risks under chronic scenarios of exposure is crucial to addressing knowledge gaps and eventually developing preventive policies. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/313097https://dx.doi.org/urn:doi:10.1021/acs.est.5c01628reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 965196Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00731Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 IJC2020-2686IEuropean Commission https://doi.org/10.13039/501100000780 801370open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3130972026-06-06T12:50:31Z
dc.title.none.fl_str_mv Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
title Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
spellingShingle Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Nanoplastics (NPLs)
Polyethylene terephthalate (PET)
Carcinogenicity
Respiratory toxicity
Chronic exposure
New approach methodologies (NAMs)
Human health risk
title_short Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
title_full Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
title_fullStr Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
title_full_unstemmed Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
title_sort Long-Term Exposure to Real-Life Polyethylene Terephthalate Nanoplastics Induces Carcinogenesis In Vitro
dc.creator.none.fl_str_mv Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Barguilla, Irene|||0000-0003-1081-4457
Nymark, Penny|||0000-0002-3435-7775
García Rodríguez, Alba|||0000-0002-1175-7418
Guyot, Boris|||0000-0002-4512-7123
Maguer-Satta, Veronique|||0000-0002-1556-068X
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Rubio Lorente, Laura|||0000-0002-2088-3803
Hernández Bonilla, Alba|||0000-0001-6938-1233
author Gutierrez Garcia, Javier|||0009-0007-7376-8933
author_facet Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Barguilla, Irene|||0000-0003-1081-4457
Nymark, Penny|||0000-0002-3435-7775
García Rodríguez, Alba|||0000-0002-1175-7418
Guyot, Boris|||0000-0002-4512-7123
Maguer-Satta, Veronique|||0000-0002-1556-068X
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Rubio Lorente, Laura|||0000-0002-2088-3803
Hernández Bonilla, Alba|||0000-0001-6938-1233
author_role author
author2 Egea, Raquel|||0000-0002-6201-4112
Barguilla, Irene|||0000-0003-1081-4457
Nymark, Penny|||0000-0002-3435-7775
García Rodríguez, Alba|||0000-0002-1175-7418
Guyot, Boris|||0000-0002-4512-7123
Maguer-Satta, Veronique|||0000-0002-1556-068X
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Rubio Lorente, Laura|||0000-0002-2088-3803
Hernández Bonilla, Alba|||0000-0001-6938-1233
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanoplastics (NPLs)
Polyethylene terephthalate (PET)
Carcinogenicity
Respiratory toxicity
Chronic exposure
New approach methodologies (NAMs)
Human health risk
topic Nanoplastics (NPLs)
Polyethylene terephthalate (PET)
Carcinogenicity
Respiratory toxicity
Chronic exposure
New approach methodologies (NAMs)
Human health risk
description Micro/nanoplastics (MNPLs) are environmental contaminants originating mainly from plastic waste degradation that pose potential health risks. Inhalation is a major exposure route, as evidenced by their detection in human lungs, with polyethylene terephthalate (PET) among the most abundant particles in respiratory airways. However, the harmful effects of particle bioaccumulation remain unclear, as chronic effects are understudied. To assess long-term effects, specifically carcinogenic effects, BEAS-2B cells were exposed to PET-NPLs for 30 weeks. Genotoxicity, carcinogenic phenotypic hallmarks, and a panel of genes and pathways associated with cell transformation and lung cancer were examined and compared across three exposure durations. No significant effects were observed after 24 h or 15 weeks of exposure. However, a 30-week exposure led to increased genotoxic damage, anchorage-independent growth, and invasive potential. Transcriptomic analysis showed the upregulation of several oncogenes and lung cancer-associated genes at the end of the exposure. Further analysis revealed an increase in differentially expressed genes over time and a temporal gradient of lung cancer-related genes. Altogether, the data suggest PET-NPLs' potential carcinogenicity after extended exposure, highlighting serious long-term health risks of MNPLs. Assessing their carcinogenic risks under chronic scenarios of exposure is crucial to addressing knowledge gaps and eventually developing preventive policies.
publishDate 2025
dc.date.none.fl_str_mv 2
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/313097
https://dx.doi.org/urn:doi:10.1021/acs.est.5c01628
url https://ddd.uab.cat/record/313097
https://dx.doi.org/urn:doi:10.1021/acs.est.5c01628
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 965196
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00731
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 IJC2020-2686I
European Commission https://doi.org/10.13039/501100000780 801370
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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