Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency

The nanomechanical response of a cell depends on the frequency at which thecell is probed. The components of the cell that contribute to this property andtheir interplay are not well understood. Here, two force microscopy methodsare integrated to characterize the frequency and/or the velocity-depend...

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Autores: Gisbert, Victor G., Espinosa, Francisco M., Sánchez, Juan G., Serrano, María C., García García, Ricardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/423744
Acceso en línea:http://hdl.handle.net/10261/423744
Access Level:acceso abierto
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spelling Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the FrequencyGisbert, Victor G.Espinosa, Francisco M.Sánchez, Juan G.Serrano, María C.García García, RicardoThe nanomechanical response of a cell depends on the frequency at which thecell is probed. The components of the cell that contribute to this property andtheir interplay are not well understood. Here, two force microscopy methodsare integrated to characterize the frequency and/or the velocity-dependentproperties of living cells. It is shown on HeLa and fibroblasts, that cells softenand fluidize upon increasing the frequency or the velocity of the deformation.This property was independent of the type and values (25 or 1000 nm) of thedeformation. At low frequencies (2-10 Hz) or velocities (1–10 μm s−1 ), theresponse is dominated by the mechanical properties of the cell surface. Athigher frequencies (>10 Hz) or velocities (>10 μm s−1 ), the response isdominated by the hydrodynamic drag of the cytosol. Softening andfluidization does not seem to involve any structural remodeling. It reflects aredistribution of the applied stress between the solid and liquid-like elementsof the cell as the frequency or the velocity is changed. The data indicates thatthe quasistatic mechanical properties of a cell featuring a cytoskeletonpathology might be mimicked by the response of a non-pathological cellwhich is probed at a high frequency.V.G.G. and F.M.E. contributed equally to this work. Financial sup-port from the Ministerio de Ciencia e Innovación (PID2019-106801GB-I00 /AEI/10.13039/501100011033), CSIC 202050E013, Comunidad de Madrid S2018/NMT-4443 (Tec4Bio-CM) and the European Commission(HORIZON-EIC-2022, project Piezo4Spine, No. 101098597) are acknowl-edged.Ministerio de Ciencia e Innovación (España); Agencia Estatal de Investigación (España); Comunidad de Madrid; European CommissionJohn Wiley & SonsConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2026202620232026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/423744reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106801GB-I00info:eu-repo/grantAgreement///S2018info:eu-repo/grantAgreement/EC/HE/101098597https://doi.org/10.1002/smll.202304884Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4237442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
title Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
spellingShingle Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
Gisbert, Victor G.
title_short Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
title_full Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
title_fullStr Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
title_full_unstemmed Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
title_sort Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
dc.creator.none.fl_str_mv Gisbert, Victor G.
Espinosa, Francisco M.
Sánchez, Juan G.
Serrano, María C.
García García, Ricardo
author Gisbert, Victor G.
author_facet Gisbert, Victor G.
Espinosa, Francisco M.
Sánchez, Juan G.
Serrano, María C.
García García, Ricardo
author_role author
author2 Espinosa, Francisco M.
Sánchez, Juan G.
Serrano, María C.
García García, Ricardo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description The nanomechanical response of a cell depends on the frequency at which thecell is probed. The components of the cell that contribute to this property andtheir interplay are not well understood. Here, two force microscopy methodsare integrated to characterize the frequency and/or the velocity-dependentproperties of living cells. It is shown on HeLa and fibroblasts, that cells softenand fluidize upon increasing the frequency or the velocity of the deformation.This property was independent of the type and values (25 or 1000 nm) of thedeformation. At low frequencies (2-10 Hz) or velocities (1–10 μm s−1 ), theresponse is dominated by the mechanical properties of the cell surface. Athigher frequencies (>10 Hz) or velocities (>10 μm s−1 ), the response isdominated by the hydrodynamic drag of the cytosol. Softening andfluidization does not seem to involve any structural remodeling. It reflects aredistribution of the applied stress between the solid and liquid-like elementsof the cell as the frequency or the velocity is changed. The data indicates thatthe quasistatic mechanical properties of a cell featuring a cytoskeletonpathology might be mimicked by the response of a non-pathological cellwhich is probed at a high frequency.
publishDate 2023
dc.date.none.fl_str_mv 2023
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/423744
url http://hdl.handle.net/10261/423744
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106801GB-I00
info:eu-repo/grantAgreement///S2018
info:eu-repo/grantAgreement/EC/HE/101098597
https://doi.org/10.1002/smll.202304884
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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