Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression

Background: The yeast Xanthophyllomyces dendrorhous produces carotenoids of commercial interest, including astaxanthin and β-carotene. Although carotenogenesis in this yeast and the expression profiles of the genes controlling this pathway are known, the mechanisms regulating this process remain poo...

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Autores: Cordova, Pamela, Alcaíno, Jennifer, Bravo, Natalia, Barahona, Salvador, Sepúlveda, Dionisia, Fernández Lobato, María, Baeza, Marcelo, Cifuentes, Víctor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/152059
Acceso en línea:http://hdl.handle.net/10261/152059
Access Level:acceso abierto
Palabra clave:Catabolic repression
Carotenogenesis
Transcriptional regulation
Xanthophyllomyces dendrorhous
Cyc8–Tup1 co-repressor complex
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spelling Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repressionCordova, PamelaAlcaíno, JenniferBravo, NataliaBarahona, SalvadorSepúlveda, DionisiaFernández Lobato, MaríaBaeza, MarceloCifuentes, VíctorCatabolic repressionCarotenogenesisTranscriptional regulationXanthophyllomyces dendrorhousCyc8–Tup1 co-repressor complexBackground: The yeast Xanthophyllomyces dendrorhous produces carotenoids of commercial interest, including astaxanthin and β-carotene. Although carotenogenesis in this yeast and the expression profiles of the genes controlling this pathway are known, the mechanisms regulating this process remain poorly understood. Several studies have demonstrated that glucose represses carotenogenesis in X. dendrorhous, suggesting that this pathway could be regulated by catabolic repression. Catabolic repression is a highly conserved regulatory mechanism in eukaryotes and has been widely studied in Saccharomyces cerevisiae. Glucose-dependent repression is mainly observed at the transcriptional level and depends on the DNA-binding regulator Mig1, which recruits the co-repressor complex Cyc8-Tup1, which then represses the expression of target genes. In this work, we studied the regulation of carotenogenesis by catabolic repression in X. dendrorhous, focusing on the role of the co-repressor complex Cyc8-Tup1. Results: The X. dendrorhous CYC8 and TUP1 genes were identified, and their functions were demonstrated by heterologous complementation in S. cerevisiae. In addition, cyc8 and tup1 mutant strains of X. dendrorhous were obtained, and both mutations were shown to prevent the glucose-dependent repression of carotenogenesis in X. dendrorhous, increasing the carotenoid production in both mutant strains. Furthermore, the effects of glucose on the transcript levels of genes involved in carotenogenesis differed between the mutant strains and wild-type X. dendrorhous, particularly for genes involved in the synthesis of carotenoid precursors, such as HMGR, idi and FPS. Additionally, transcriptomic analyses showed that cyc8 and tup1 mutations affected the expression of over 250 genes in X. dendrorhous. Conclusions: The CYC8 and TUP1 genes are functional in X. dendrorhous, and their gene products are involved in catabolic repression and carotenogenesis regulation. This study presents the first report involving the participation of Cyc8 and Tup1 in carotenogenesis regulation in yeast.FONDECYT 1140504 and by a graduate scholarships CONICYT 21110252 to PC and MECESUP 2-UCH0604 to NBPeer ReviewedBioMed CentralComisión Nacional de Investigación Científica y Tecnológica (Chile)Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/152059reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1520592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
title Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
spellingShingle Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
Cordova, Pamela
Catabolic repression
Carotenogenesis
Transcriptional regulation
Xanthophyllomyces dendrorhous
Cyc8–Tup1 co-repressor complex
title_short Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
title_full Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
title_fullStr Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
title_full_unstemmed Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
title_sort Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: The role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression
dc.creator.none.fl_str_mv Cordova, Pamela
Alcaíno, Jennifer
Bravo, Natalia
Barahona, Salvador
Sepúlveda, Dionisia
Fernández Lobato, María
Baeza, Marcelo
Cifuentes, Víctor
author Cordova, Pamela
author_facet Cordova, Pamela
Alcaíno, Jennifer
Bravo, Natalia
Barahona, Salvador
Sepúlveda, Dionisia
Fernández Lobato, María
Baeza, Marcelo
Cifuentes, Víctor
author_role author
author2 Alcaíno, Jennifer
Bravo, Natalia
Barahona, Salvador
Sepúlveda, Dionisia
Fernández Lobato, María
Baeza, Marcelo
Cifuentes, Víctor
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comisión Nacional de Investigación Científica y Tecnológica (Chile)
Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Catabolic repression
Carotenogenesis
Transcriptional regulation
Xanthophyllomyces dendrorhous
Cyc8–Tup1 co-repressor complex
topic Catabolic repression
Carotenogenesis
Transcriptional regulation
Xanthophyllomyces dendrorhous
Cyc8–Tup1 co-repressor complex
description Background: The yeast Xanthophyllomyces dendrorhous produces carotenoids of commercial interest, including astaxanthin and β-carotene. Although carotenogenesis in this yeast and the expression profiles of the genes controlling this pathway are known, the mechanisms regulating this process remain poorly understood. Several studies have demonstrated that glucose represses carotenogenesis in X. dendrorhous, suggesting that this pathway could be regulated by catabolic repression. Catabolic repression is a highly conserved regulatory mechanism in eukaryotes and has been widely studied in Saccharomyces cerevisiae. Glucose-dependent repression is mainly observed at the transcriptional level and depends on the DNA-binding regulator Mig1, which recruits the co-repressor complex Cyc8-Tup1, which then represses the expression of target genes. In this work, we studied the regulation of carotenogenesis by catabolic repression in X. dendrorhous, focusing on the role of the co-repressor complex Cyc8-Tup1. Results: The X. dendrorhous CYC8 and TUP1 genes were identified, and their functions were demonstrated by heterologous complementation in S. cerevisiae. In addition, cyc8 and tup1 mutant strains of X. dendrorhous were obtained, and both mutations were shown to prevent the glucose-dependent repression of carotenogenesis in X. dendrorhous, increasing the carotenoid production in both mutant strains. Furthermore, the effects of glucose on the transcript levels of genes involved in carotenogenesis differed between the mutant strains and wild-type X. dendrorhous, particularly for genes involved in the synthesis of carotenoid precursors, such as HMGR, idi and FPS. Additionally, transcriptomic analyses showed that cyc8 and tup1 mutations affected the expression of over 250 genes in X. dendrorhous. Conclusions: The CYC8 and TUP1 genes are functional in X. dendrorhous, and their gene products are involved in catabolic repression and carotenogenesis regulation. This study presents the first report involving the participation of Cyc8 and Tup1 in carotenogenesis regulation in yeast.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/152059
url http://hdl.handle.net/10261/152059
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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