A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components

[EN] Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated in the plant response to the drought stress hormone abscisic acid (ABA). T...

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Autores: Peirats-Llobet, Marta, Han, Soon-Ki, González Guzmán, Miguel, Jeong, Cheol Woong, Rodríguez Solovey, Leisa Natacha, Wagner, Doris, Belda Palazón, Borja, Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/82417
Acceso en línea:https://riunet.upv.es/handle/10251/82417
Access Level:acceso abierto
Palabra clave:Abscisic acid
Hormone signaling
Chromatin remodeling
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
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spelling A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway ComponentsPeirats-Llobet, MartaHan, Soon-KiGonzález Guzmán, MiguelJeong, Cheol WoongRodríguez Solovey, Leisa NatachaWagner, DorisBelda Palazón, BorjaRodríguez Egea, Pedro Luís|||0000-0002-5886-9425Abscisic acidHormone signalingChromatin remodelingMICROBIOLOGIABIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated in the plant response to the drought stress hormone abscisic acid (ABA). The Arabidopsis SWI/SNF chromatin-remodeling ATPase BRAHMA (BRM) modulates response to ABA by preventing premature activation of stress response pathways during germination. We show that core ABA signaling pathway components physically interact with BRM and post-translationally modify BRM by phosphorylation/dephosphorylation. Genetic evidence suggests that BRM acts downstream of SnRK2.2/2.3 kinases, and biochemical studies identified phosphorylation sites in the C-terminal region of BRM at SnRK2 target sites that are evolutionarily conserved. Finally, the phosphomimetic BRMS1760D (S1762D) mutant displays ABA hypersensitivity. Prior studies showed that BRM resides at target loci in the ABA pathway in the presence and absence of the stimulus, but is only active in the absence of ABA. Our data suggest that SnRK2-dependent phosphorylation of BRM leads to its inhibition, and PP2CA-mediated dephosphorylation of BRM restores the ability of BRM to repress ABA response. These findings point to the presence of a rapid phosphorylation-based switch to control BRM activity; this property could be potentially harnessed to improve drought tolerance in plants.Work in Dr. Rodriguez's laboratory was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas (grant BIO2014-52537-R). M.P.-L. and L.R. were supported by FPI fellowships, and M.G.-G. by a JAE-DOC research contract. Funding of chromatin research in the Wagner laboratory is supported by National Science Foundation grant MCB-0925071.Oxford University Press (OUP)Instituto Universitario Mixto de Biología Molecular y Celular de PlantasMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-01-04journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/82417reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2014-52537-R REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIAopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/824172026-06-13T07:49:27Z
dc.title.none.fl_str_mv A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
title A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
spellingShingle A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
Peirats-Llobet, Marta
Abscisic acid
Hormone signaling
Chromatin remodeling
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
title_full A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
title_fullStr A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
title_full_unstemmed A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
title_sort A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components
dc.creator.none.fl_str_mv Peirats-Llobet, Marta
Han, Soon-Ki
González Guzmán, Miguel
Jeong, Cheol Woong
Rodríguez Solovey, Leisa Natacha
Wagner, Doris
Belda Palazón, Borja
Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
author Peirats-Llobet, Marta
author_facet Peirats-Llobet, Marta
Han, Soon-Ki
González Guzmán, Miguel
Jeong, Cheol Woong
Rodríguez Solovey, Leisa Natacha
Wagner, Doris
Belda Palazón, Borja
Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
author_role author
author2 Han, Soon-Ki
González Guzmán, Miguel
Jeong, Cheol Woong
Rodríguez Solovey, Leisa Natacha
Wagner, Doris
Belda Palazón, Borja
Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Abscisic acid
Hormone signaling
Chromatin remodeling
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Abscisic acid
Hormone signaling
Chromatin remodeling
MICROBIOLOGIA
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated in the plant response to the drought stress hormone abscisic acid (ABA). The Arabidopsis SWI/SNF chromatin-remodeling ATPase BRAHMA (BRM) modulates response to ABA by preventing premature activation of stress response pathways during germination. We show that core ABA signaling pathway components physically interact with BRM and post-translationally modify BRM by phosphorylation/dephosphorylation. Genetic evidence suggests that BRM acts downstream of SnRK2.2/2.3 kinases, and biochemical studies identified phosphorylation sites in the C-terminal region of BRM at SnRK2 target sites that are evolutionarily conserved. Finally, the phosphomimetic BRMS1760D (S1762D) mutant displays ABA hypersensitivity. Prior studies showed that BRM resides at target loci in the ABA pathway in the presence and absence of the stimulus, but is only active in the absence of ABA. Our data suggest that SnRK2-dependent phosphorylation of BRM leads to its inhibition, and PP2CA-mediated dephosphorylation of BRM restores the ability of BRM to repress ABA response. These findings point to the presence of a rapid phosphorylation-based switch to control BRM activity; this property could be potentially harnessed to improve drought tolerance in plants.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-04
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/82417
url https://riunet.upv.es/handle/10251/82417
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2014-52537-R REGULACION DE LA SEÑALIZACION DEL ABA MEDIANTE MECHANISMOS QUE AFECTAN LOCALIZACION SUBCELULAR, VIDA MEDIA Y ACTIVIDAD DE RECEPTORES PARA REFORZAR TOLERANCIA VEGETAL A SEQUIA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press (OUP)
publisher.none.fl_str_mv Oxford University Press (OUP)
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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