Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies

Plants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory...

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Autores: Zaragoza-Puchol, Daniel, Ortiz, Javier E., Orden, Alejandro A., Sánchez, Marianela, Palermo, Jorge, Tapia, Alejandro, Bastida Armengol, Jaume, Feresin, Gabriela E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/184412
Acceso en línea:https://hdl.handle.net/2445/184412
Access Level:acceso abierto
Palabra clave:Amaril·lidàcies
Alcaloides
Compostos bioactius
Amaryllidaceae
Alkaloids
Bioactive compounds
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spelling Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation StrategiesZaragoza-Puchol, DanielOrtiz, Javier E.Orden, Alejandro A.Sánchez, MarianelaPalermo, JorgeTapia, AlejandroBastida Armengol, JaumeFeresin, Gabriela E.Amaril·lidàciesAlcaloidesCompostos bioactiusAmaryllidaceaeAlkaloidsBioactive compoundsPlants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory activity against cholinesterases, and to test several propagation strategies for biomass production. Eleven compounds were characterized by GC-MS in the alkaloid extract, which showed a relatively high proportion of tazettine. The known alkaloids tazettine, haemanthamine, and the epimer mixture haemanthidine/6-epi-haemanthidine were isolated and identified by spectroscopic methods. Inhibitory cholinesterases activity was not detected. Three forms of propagation were performed: bulb propagation from seed, cut-induced bulb division, and micropropagated bulbs. Finally, different imbibition and post-collection times were evaluated in seed germination assays. The best propagation method was cut-induced bulb division with longitudinal cuts into quarters (T1) while the best conditions for seed germination were 0-day of post-collection and two days of imbibition. The alkaloids analyses of the H. cardenasianus bulbs showed that they are a source of anti-tumoral alkaloids, especially pretazettine (tazettine) and T1 is a sustainable strategy for its propagation and domestication to produce bioactive alkaloids. Keywords: Amaryllidaceae; bioactive alkaloids; GC-MS; propagation methods; biomass productionMDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/184412Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/molecules26010192Molecules, 2021, vol. 26, num. 1, p. 192https://doi.org/10.3390/molecules26010192cc-by (c) Zaragoza-Puchol, Daniel et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1844122026-05-27T06:46:51Z
dc.title.none.fl_str_mv Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
title Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
spellingShingle Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
Zaragoza-Puchol, Daniel
Amaril·lidàcies
Alcaloides
Compostos bioactius
Amaryllidaceae
Alkaloids
Bioactive compounds
title_short Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
title_full Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
title_fullStr Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
title_full_unstemmed Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
title_sort Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies
dc.creator.none.fl_str_mv Zaragoza-Puchol, Daniel
Ortiz, Javier E.
Orden, Alejandro A.
Sánchez, Marianela
Palermo, Jorge
Tapia, Alejandro
Bastida Armengol, Jaume
Feresin, Gabriela E.
author Zaragoza-Puchol, Daniel
author_facet Zaragoza-Puchol, Daniel
Ortiz, Javier E.
Orden, Alejandro A.
Sánchez, Marianela
Palermo, Jorge
Tapia, Alejandro
Bastida Armengol, Jaume
Feresin, Gabriela E.
author_role author
author2 Ortiz, Javier E.
Orden, Alejandro A.
Sánchez, Marianela
Palermo, Jorge
Tapia, Alejandro
Bastida Armengol, Jaume
Feresin, Gabriela E.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Amaril·lidàcies
Alcaloides
Compostos bioactius
Amaryllidaceae
Alkaloids
Bioactive compounds
topic Amaril·lidàcies
Alcaloides
Compostos bioactius
Amaryllidaceae
Alkaloids
Bioactive compounds
description Plants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory activity against cholinesterases, and to test several propagation strategies for biomass production. Eleven compounds were characterized by GC-MS in the alkaloid extract, which showed a relatively high proportion of tazettine. The known alkaloids tazettine, haemanthamine, and the epimer mixture haemanthidine/6-epi-haemanthidine were isolated and identified by spectroscopic methods. Inhibitory cholinesterases activity was not detected. Three forms of propagation were performed: bulb propagation from seed, cut-induced bulb division, and micropropagated bulbs. Finally, different imbibition and post-collection times were evaluated in seed germination assays. The best propagation method was cut-induced bulb division with longitudinal cuts into quarters (T1) while the best conditions for seed germination were 0-day of post-collection and two days of imbibition. The alkaloids analyses of the H. cardenasianus bulbs showed that they are a source of anti-tumoral alkaloids, especially pretazettine (tazettine) and T1 is a sustainable strategy for its propagation and domestication to produce bioactive alkaloids. Keywords: Amaryllidaceae; bioactive alkaloids; GC-MS; propagation methods; biomass production
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184412
url https://hdl.handle.net/2445/184412
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/molecules26010192
Molecules, 2021, vol. 26, num. 1, p. 192
https://doi.org/10.3390/molecules26010192
dc.rights.none.fl_str_mv cc-by (c) Zaragoza-Puchol, Daniel et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Zaragoza-Puchol, Daniel et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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