Pain indicators in lobsters applying omics

Trabajo final presentado por Sandra López Chillarón para el Master in Genetics and Genomics de la Universitat de Barcelona (UB), realizado bajo la dirección de la Dra. Guiomar Rotllant Estelrich y la Dra. Noelia Díaz Blanco del Institut de Ciències del Mar (ICM-CSIC).-- 82 pages, 46 figures, 3 table...

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Detalles Bibliográficos
Autor: López Chillarón, Sandra
Tipo de recurso: tesis de maestría
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/380161
Acceso en línea:http://hdl.handle.net/10261/380161
Access Level:acceso abierto
Palabra clave:Homarus americanus
Lobster
Welfare
Pain
Molecular pain indicators
Central nervous system
Omics
Transcriptome
Differentially expressed genes
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
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spelling Pain indicators in lobsters applying omicsLópez Chillarón, SandraHomarus americanusLobsterWelfarePainMolecular pain indicatorsCentral nervous systemOmicsTranscriptomeDifferentially expressed geneshttp://metadata.un.org/sdg/14Conserve and sustainably use the oceans, seas and marine resources for sustainable developmentTrabajo final presentado por Sandra López Chillarón para el Master in Genetics and Genomics de la Universitat de Barcelona (UB), realizado bajo la dirección de la Dra. Guiomar Rotllant Estelrich y la Dra. Noelia Díaz Blanco del Institut de Ciències del Mar (ICM-CSIC).-- 82 pages, 46 figures, 3 tablesHomarus americanus, a decapod crustacean, faces significant pressures from fisheries to consumption. It is commercially valuable and widely imported from the American continent to Europe. Decapod crustaceans’ welfare has become a topic of debate since there is evidence of their capacity to feel pain. This study aims to address the well-being of these individuals by analysing the central nervous system (CNS) and finding potential pain indicators. We constructed a transcriptome from the CNS tissues (eyestalk-Ey, brain-Br, circumesophageal connectives-CC, suboesophageal ganglia-SOG, thoracic ganglia-TG, and abdominal ganglia- AG) of adult male lobsters. Our analysis compares CNS transcripts across four treatment groups: control group (C), lobsters subjected to a negative stimulus (C-E), stunned lobsters (TL), and stunned lobsters that also are subjected to a negative stimulus (TL-E). We hypothesized that Ey, Br, and SOG would present a higher number of differentially expressed genes (DEGs) and that DEGs might correspond to certain genes encoding CHH and neurotransmitters such as dopamine, GABA, acetylcholine, allatostatin, and serotonin, along with elements involved in their pathways. Our initial global analysis revealed no significant differences among the six CNS tissues regarding the number DEGs. In the treatment-focused comparisons, more DEGs were observed in the TL-EvsC comparison, with a notable reduction in rRNA expression in the TL-EvsC and TLvsC compared to C-EvsC. Our findings suggest as a potential pain-related genes: RASD2 (a dopamine modulator), FUT1, VCAN, ACE, ACTC1, RPL34, NPTX1, HMCN2, PKHD1L1, ITGA5, and SLC17A5. This study is the first that explores transcriptomic changes in lobsters under stunning and negative stimuli treatments, presenting the first list of potential pain indicatorsWith the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewedUniversidad de BarcelonaCSIC - Instituto de Ciencias del Mar (ICM)Rotllant, GuiomarDíaz, NoeliaAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/masterThesishttp://purl.org/coar/resource_type/c_bdcchttp://hdl.handle.net/10261/380161reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3801612026-05-22T06:33:51Z
dc.title.none.fl_str_mv Pain indicators in lobsters applying omics
title Pain indicators in lobsters applying omics
spellingShingle Pain indicators in lobsters applying omics
López Chillarón, Sandra
Homarus americanus
Lobster
Welfare
Pain
Molecular pain indicators
Central nervous system
Omics
Transcriptome
Differentially expressed genes
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
title_short Pain indicators in lobsters applying omics
title_full Pain indicators in lobsters applying omics
title_fullStr Pain indicators in lobsters applying omics
title_full_unstemmed Pain indicators in lobsters applying omics
title_sort Pain indicators in lobsters applying omics
dc.creator.none.fl_str_mv López Chillarón, Sandra
author López Chillarón, Sandra
author_facet López Chillarón, Sandra
author_role author
dc.contributor.none.fl_str_mv Rotllant, Guiomar
Díaz, Noelia
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Homarus americanus
Lobster
Welfare
Pain
Molecular pain indicators
Central nervous system
Omics
Transcriptome
Differentially expressed genes
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
topic Homarus americanus
Lobster
Welfare
Pain
Molecular pain indicators
Central nervous system
Omics
Transcriptome
Differentially expressed genes
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
description Trabajo final presentado por Sandra López Chillarón para el Master in Genetics and Genomics de la Universitat de Barcelona (UB), realizado bajo la dirección de la Dra. Guiomar Rotllant Estelrich y la Dra. Noelia Díaz Blanco del Institut de Ciències del Mar (ICM-CSIC).-- 82 pages, 46 figures, 3 tables
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/masterThesis
http://purl.org/coar/resource_type/c_bdcc
format masterThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/380161
url http://hdl.handle.net/10261/380161
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidad de Barcelona
CSIC - Instituto de Ciencias del Mar (ICM)
publisher.none.fl_str_mv Universidad de Barcelona
CSIC - Instituto de Ciencias del Mar (ICM)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411649722515456
score 15,811543

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