Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia

Background: Rehabilitation therapy is the only available treatment for stroke survivors presenting neurological deficits; however, the underlying molecules and mechanisms associated with functional/motor improvement during rehabilitation are poorly understood. Objective: Our aim is to study the modu...

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Autores: Gabriel Salazar, Marina|||0000-0002-7521-1502, Morancho, Anna|||0000-0001-9059-3206, Rodriguez, Susana|||0000-0002-6170-5253, Buxo Masip, Xavier|||0000-0001-6814-7373, García-Rodríguez, Nicolás, Colell, Guillem, Fernandez, Albert, Giralt, Dolors, Bustamante, Alejandro|||0000-0003-4390-8900, Montaner, Joan|||0000-0003-4845-2279, Rosell Novel, Anna|||0000-0003-1082-3599
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:253589
Acceso en línea:https://ddd.uab.cat/record/253589
https://dx.doi.org/urn:doi:10.3389/fneur.2018.00508
Access Level:acceso abierto
Palabra clave:Stroke
Rehabilitation
Angiogenesis
Angiogenin
Endothelial progenitor cell
Biomarker
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spelling Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral IschemiaGabriel Salazar, Marina|||0000-0002-7521-1502Morancho, Anna|||0000-0001-9059-3206Rodriguez, Susana|||0000-0002-6170-5253Buxo Masip, Xavier|||0000-0001-6814-7373García-Rodríguez, NicolásColell, GuillemFernandez, AlbertGiralt, DolorsBustamante, Alejandro|||0000-0003-4390-8900Montaner, Joan|||0000-0003-4845-2279Rosell Novel, Anna|||0000-0003-1082-3599StrokeRehabilitationAngiogenesisAngiogeninEndothelial progenitor cellBiomarkerBackground: Rehabilitation therapy is the only available treatment for stroke survivors presenting neurological deficits; however, the underlying molecules and mechanisms associated with functional/motor improvement during rehabilitation are poorly understood. Objective: Our aim is to study the modulation of angiogenin and endothelial progenitor cells (EPCs) as repair-associated factors in a cohort of stroke patients and mouse models of rehabilitation after cerebral ischemia. Methods: The clinical study included 18 ischemic strokes admitted to an intensive rehabilitation therapy (IRT) unit, 18 non-ischemic controls and brain samples from three deceased patients. Angiogenin and EPCs were measured in blood obtained before and up to 6 months after IRT together with an extensive evaluation of the motor/functional status. In parallel, C57BL/6 mice underwent middle cerebral artery occlusion, and the pasta matrix reaching-task or treadmill exercises were used as rehabilitation models. Angiogenin RNA expression was measured after 2 or 12 days of treatment together with cell counts from EPCs cultures. Results: Brain angiogenin was identified in both human and mouse tissue, whereas serum levels increased after 1 month of IRT in association with motor/functional improvement. EPC populations were increased after stroke and remained elevated during follow-up after IRT. The mouse model of rehabilitation by the task-specific pasta matrix exercise increased the number of EPCs at 2 days and increased angiogenin expression after 12 days of rehabilitation. Conclusions: Angiogenin and EPCs are modulated by rehabilitation after cerebral ischemia, suggesting that both angiogenin and EPCs could serve as biomarkers of improvement during rehabilitation or future therapeutic targetsUniversitat Autònoma de Barcelona 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/253589https://dx.doi.org/urn:doi:10.3389/fneur.2018.00508reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 JR16/00008Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 II15/00003Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI13/01094Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI16/00981Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD12/0014/0005open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2535892026-06-06T12:50:31Z
dc.title.none.fl_str_mv Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
title Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
spellingShingle Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
Gabriel Salazar, Marina|||0000-0002-7521-1502
Stroke
Rehabilitation
Angiogenesis
Angiogenin
Endothelial progenitor cell
Biomarker
title_short Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
title_full Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
title_fullStr Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
title_full_unstemmed Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
title_sort Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia
dc.creator.none.fl_str_mv Gabriel Salazar, Marina|||0000-0002-7521-1502
Morancho, Anna|||0000-0001-9059-3206
Rodriguez, Susana|||0000-0002-6170-5253
Buxo Masip, Xavier|||0000-0001-6814-7373
García-Rodríguez, Nicolás
Colell, Guillem
Fernandez, Albert
Giralt, Dolors
Bustamante, Alejandro|||0000-0003-4390-8900
Montaner, Joan|||0000-0003-4845-2279
Rosell Novel, Anna|||0000-0003-1082-3599
author Gabriel Salazar, Marina|||0000-0002-7521-1502
author_facet Gabriel Salazar, Marina|||0000-0002-7521-1502
Morancho, Anna|||0000-0001-9059-3206
Rodriguez, Susana|||0000-0002-6170-5253
Buxo Masip, Xavier|||0000-0001-6814-7373
García-Rodríguez, Nicolás
Colell, Guillem
Fernandez, Albert
Giralt, Dolors
Bustamante, Alejandro|||0000-0003-4390-8900
Montaner, Joan|||0000-0003-4845-2279
Rosell Novel, Anna|||0000-0003-1082-3599
author_role author
author2 Morancho, Anna|||0000-0001-9059-3206
Rodriguez, Susana|||0000-0002-6170-5253
Buxo Masip, Xavier|||0000-0001-6814-7373
García-Rodríguez, Nicolás
Colell, Guillem
Fernandez, Albert
Giralt, Dolors
Bustamante, Alejandro|||0000-0003-4390-8900
Montaner, Joan|||0000-0003-4845-2279
Rosell Novel, Anna|||0000-0003-1082-3599
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Stroke
Rehabilitation
Angiogenesis
Angiogenin
Endothelial progenitor cell
Biomarker
topic Stroke
Rehabilitation
Angiogenesis
Angiogenin
Endothelial progenitor cell
Biomarker
description Background: Rehabilitation therapy is the only available treatment for stroke survivors presenting neurological deficits; however, the underlying molecules and mechanisms associated with functional/motor improvement during rehabilitation are poorly understood. Objective: Our aim is to study the modulation of angiogenin and endothelial progenitor cells (EPCs) as repair-associated factors in a cohort of stroke patients and mouse models of rehabilitation after cerebral ischemia. Methods: The clinical study included 18 ischemic strokes admitted to an intensive rehabilitation therapy (IRT) unit, 18 non-ischemic controls and brain samples from three deceased patients. Angiogenin and EPCs were measured in blood obtained before and up to 6 months after IRT together with an extensive evaluation of the motor/functional status. In parallel, C57BL/6 mice underwent middle cerebral artery occlusion, and the pasta matrix reaching-task or treadmill exercises were used as rehabilitation models. Angiogenin RNA expression was measured after 2 or 12 days of treatment together with cell counts from EPCs cultures. Results: Brain angiogenin was identified in both human and mouse tissue, whereas serum levels increased after 1 month of IRT in association with motor/functional improvement. EPC populations were increased after stroke and remained elevated during follow-up after IRT. The mouse model of rehabilitation by the task-specific pasta matrix exercise increased the number of EPCs at 2 days and increased angiogenin expression after 12 days of rehabilitation. Conclusions: Angiogenin and EPCs are modulated by rehabilitation after cerebral ischemia, suggesting that both angiogenin and EPCs could serve as biomarkers of improvement during rehabilitation or future therapeutic targets
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/253589
https://dx.doi.org/urn:doi:10.3389/fneur.2018.00508
url https://ddd.uab.cat/record/253589
https://dx.doi.org/urn:doi:10.3389/fneur.2018.00508
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 JR16/00008
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 II15/00003
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI13/01094
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI16/00981
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD12/0014/0005
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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