International external quality control assessment for the serological diagnosis of dengue infections

BACKGROUND: Dengue is endemic to the tropics and subtropics, and the most frequent of arthropod-borne viral diseases. Reliable diagnosis of dengue infection is important not only in clinical care but also in disease surveillance, the control of outbreaks, and the development of new vaccines. The dia...

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Autores: Domingo Carrasco, Cristina, Alves, María Joao, De Ory, Fernando de, Teichmann, Anette, Schmitz, Herbert, Müller, Rolf, Niedrig, Matthias
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/4810
Acceso en línea:http://hdl.handle.net/20.500.12105/4810
Access Level:acceso abierto
Palabra clave:Dengue
Serology
External quality assessment
Dengue IgM
Dengue IgG
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spelling International external quality control assessment for the serological diagnosis of dengue infectionsDomingo Carrasco, CristinaAlves, María JoaoDe Ory, Fernando deTeichmann, AnetteSchmitz, HerbertMüller, RolfNiedrig, MatthiasDengueSerologyExternal quality assessmentDengue IgMDengue IgGBACKGROUND: Dengue is endemic to the tropics and subtropics, and the most frequent of arthropod-borne viral diseases. Reliable diagnosis of dengue infection is important not only in clinical care but also in disease surveillance, the control of outbreaks, and the development of new vaccines. The diagnosis of dengue infection is usually established by a variety of commercial or in-house serological protocols. The European Network for the Diagnostics of Imported Viral Diseases (ENIVD) recognized the need to survey the accuracy of dengue serological diagnostics in current use, and organized an external quality assurance (EQA) study of dengue serological practice in diagnostic laboratories. METHODS: A 15-sample panel, consisting of sera reactive against dengue plus specificity and negative controls, was sent to 48 laboratories for serological testing. The results returned by the participating laboratories were anonymized, scored, and subjected to comparison and statistical analysis. RESULTS: Ten laboratories rated all samples correctly with regard to IgM, and only three achieved the full score for IgG detection. The main handicaps in assay performance were suboptimal sensitivity of in-house IgM detection protocols by comparison with better-performing commercial ELISA tests, and the presence of IgG cross-reactivity with heterologous flaviviruses. Differences of detail in the methodology of dengue IgG antibody detection appear to underlie the disparities in accuracy observed between laboratories. CONCLUSION: This EQA study demonstrates that there is room for many laboratories to improve sensitivity in the detection of anti-dengue virus IgM antibodies, against the benchmark set by commercial antibody capture ELISA tests. The EQA shows also that cross-reactivity is a continuing issue, and IgG detection protocols must be optimized to increase their specificity.BioMed Central (BMC)20172017-09-0420152015-04-0120152015-04-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/4810reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/48102026-06-12T12:43:37Z
dc.title.none.fl_str_mv International external quality control assessment for the serological diagnosis of dengue infections
title International external quality control assessment for the serological diagnosis of dengue infections
spellingShingle International external quality control assessment for the serological diagnosis of dengue infections
Domingo Carrasco, Cristina
Dengue
Serology
External quality assessment
Dengue IgM
Dengue IgG
title_short International external quality control assessment for the serological diagnosis of dengue infections
title_full International external quality control assessment for the serological diagnosis of dengue infections
title_fullStr International external quality control assessment for the serological diagnosis of dengue infections
title_full_unstemmed International external quality control assessment for the serological diagnosis of dengue infections
title_sort International external quality control assessment for the serological diagnosis of dengue infections
dc.creator.none.fl_str_mv Domingo Carrasco, Cristina
Alves, María Joao
De Ory, Fernando de
Teichmann, Anette
Schmitz, Herbert
Müller, Rolf
Niedrig, Matthias
author Domingo Carrasco, Cristina
author_facet Domingo Carrasco, Cristina
Alves, María Joao
De Ory, Fernando de
Teichmann, Anette
Schmitz, Herbert
Müller, Rolf
Niedrig, Matthias
author_role author
author2 Alves, María Joao
De Ory, Fernando de
Teichmann, Anette
Schmitz, Herbert
Müller, Rolf
Niedrig, Matthias
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Dengue
Serology
External quality assessment
Dengue IgM
Dengue IgG
topic Dengue
Serology
External quality assessment
Dengue IgM
Dengue IgG
description BACKGROUND: Dengue is endemic to the tropics and subtropics, and the most frequent of arthropod-borne viral diseases. Reliable diagnosis of dengue infection is important not only in clinical care but also in disease surveillance, the control of outbreaks, and the development of new vaccines. The diagnosis of dengue infection is usually established by a variety of commercial or in-house serological protocols. The European Network for the Diagnostics of Imported Viral Diseases (ENIVD) recognized the need to survey the accuracy of dengue serological diagnostics in current use, and organized an external quality assurance (EQA) study of dengue serological practice in diagnostic laboratories. METHODS: A 15-sample panel, consisting of sera reactive against dengue plus specificity and negative controls, was sent to 48 laboratories for serological testing. The results returned by the participating laboratories were anonymized, scored, and subjected to comparison and statistical analysis. RESULTS: Ten laboratories rated all samples correctly with regard to IgM, and only three achieved the full score for IgG detection. The main handicaps in assay performance were suboptimal sensitivity of in-house IgM detection protocols by comparison with better-performing commercial ELISA tests, and the presence of IgG cross-reactivity with heterologous flaviviruses. Differences of detail in the methodology of dengue IgG antibody detection appear to underlie the disparities in accuracy observed between laboratories. CONCLUSION: This EQA study demonstrates that there is room for many laboratories to improve sensitivity in the detection of anti-dengue virus IgM antibodies, against the benchmark set by commercial antibody capture ELISA tests. The EQA shows also that cross-reactivity is a continuing issue, and IgG detection protocols must be optimized to increase their specificity.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-04-01
2015
2015-04-01
2017
2017-09-04
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/4810
url http://hdl.handle.net/20.500.12105/4810
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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