Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis

Transporters of the NRAMP family are ubiquitous metal-transition transporters, playing a key role in metal homeostasis, especially in Mn and Fe homeostasis. In this work, we report the characterization of the NRAMP family members (RiSMF1, RiSMF2, RiSMF3.1 and RiSMF3.2) of the arbuscular mycorrhizal...

Descripción completa

Detalles Bibliográficos
Autores: López-Lorca, V.M., Molina-Luzón, M. J., Ferrol González, Nuria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/278952
Acceso en línea:http://hdl.handle.net/10261/278952
Access Level:acceso abierto
Palabra clave:Arbuscular mycorrhizal fungi
Iron
Manganese
NRAMP transporters
Rhizophagus irregularis
id ES_7d12e970200e120eb0bbc7283f9d008b
oai_identifier_str oai:digital.csic.es:10261/278952
network_acronym_str ES
network_name_str España
repository_id_str
spelling Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularisLópez-Lorca, V.M.Molina-Luzón, M. J.Ferrol González, NuriaArbuscular mycorrhizal fungiIronManganeseNRAMP transportersRhizophagus irregularisTransporters of the NRAMP family are ubiquitous metal-transition transporters, playing a key role in metal homeostasis, especially in Mn and Fe homeostasis. In this work, we report the characterization of the NRAMP family members (RiSMF1, RiSMF2, RiSMF3.1 and RiSMF3.2) of the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis. Phylogenetic analysis of the NRAMP sequences of different AM fungi showed that they are classified in two groups, which probably diverged early in their evolution. Functional analyses in yeast revealed that RiSMF3.2 encodes a protein mediating Mn and Fe transport from the environment. Gene-expression analyses by RT-qPCR showed that the RiSMF genes are differentially expressed in the extraradical (ERM) and intraradical (IRM) mycelium and differentially regulated by Mn and Fe availability. Mn starvation decreased RiSMF1 transcript levels in the ERM but increased RiSMF3.1 expression in the IRM. In the ERM, RiSMF1 expression was up-regulated by Fe deficiency, suggesting a role for its encoded protein in Fe-deficiency alleviation. Expression of RiSMF3.2 in the ERM was up-regulated at the early stages of Fe toxicity but down-regulated at later stages. These data suggest a role for RiSMF3.2 not only in Fe transport but also as a sensor of high external-Fe concentrations. Both Mn-and Fe-deficient conditions affected ERM development. While Mn deficiency increased hyphal length, Fe deficiency reduced sporulation.This research was funded by Spanish MCIN/AEI/10.13039/501100011033/ and FEDER Una manera de hacer Europa, grant numbers AGL2015-67098-R and RTI2018-098756-B-I00, V.M.L.-L. was supported by grant BES-2016-078463 funded by MCIN/AEI/10.13039/501100011033 and FSE “El FSE invierte en tu futuro”Multidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/278952reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-67098-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098756-B-I00http://dx.doi.org/10.3390/jof8060592Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2789522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
title Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
spellingShingle Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
López-Lorca, V.M.
Arbuscular mycorrhizal fungi
Iron
Manganese
NRAMP transporters
Rhizophagus irregularis
title_short Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
title_full Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
title_fullStr Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
title_full_unstemmed Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
title_sort Characterization of the NRAMP gene family in the arbuscular mycorrhizal fungus Rhizophagus irregularis
dc.creator.none.fl_str_mv López-Lorca, V.M.
Molina-Luzón, M. J.
Ferrol González, Nuria
author López-Lorca, V.M.
author_facet López-Lorca, V.M.
Molina-Luzón, M. J.
Ferrol González, Nuria
author_role author
author2 Molina-Luzón, M. J.
Ferrol González, Nuria
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arbuscular mycorrhizal fungi
Iron
Manganese
NRAMP transporters
Rhizophagus irregularis
topic Arbuscular mycorrhizal fungi
Iron
Manganese
NRAMP transporters
Rhizophagus irregularis
description Transporters of the NRAMP family are ubiquitous metal-transition transporters, playing a key role in metal homeostasis, especially in Mn and Fe homeostasis. In this work, we report the characterization of the NRAMP family members (RiSMF1, RiSMF2, RiSMF3.1 and RiSMF3.2) of the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis. Phylogenetic analysis of the NRAMP sequences of different AM fungi showed that they are classified in two groups, which probably diverged early in their evolution. Functional analyses in yeast revealed that RiSMF3.2 encodes a protein mediating Mn and Fe transport from the environment. Gene-expression analyses by RT-qPCR showed that the RiSMF genes are differentially expressed in the extraradical (ERM) and intraradical (IRM) mycelium and differentially regulated by Mn and Fe availability. Mn starvation decreased RiSMF1 transcript levels in the ERM but increased RiSMF3.1 expression in the IRM. In the ERM, RiSMF1 expression was up-regulated by Fe deficiency, suggesting a role for its encoded protein in Fe-deficiency alleviation. Expression of RiSMF3.2 in the ERM was up-regulated at the early stages of Fe toxicity but down-regulated at later stages. These data suggest a role for RiSMF3.2 not only in Fe transport but also as a sensor of high external-Fe concentrations. Both Mn-and Fe-deficient conditions affected ERM development. While Mn deficiency increased hyphal length, Fe deficiency reduced sporulation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/278952
url http://hdl.handle.net/10261/278952
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2015-67098-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098756-B-I00
http://dx.doi.org/10.3390/jof8060592
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411636770504704
score 15,81155

Similares en LA Referencia