Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis

This article belongs to the Special Issue Antioxidants in Cocoa.

Detalles Bibliográficos
Autores: Toro-Uribe, Said, Ibáñez, Elena, Decker, Eric A., Villamizar-Jaimes, Arley René, López-Giraldo, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/212511
Acceso en línea:http://hdl.handle.net/10261/212511
Access Level:acceso abierto
Palabra clave:Cocoa
Polyphenols
Solid–liquid kinetic extraction
Antioxidants
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spelling Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysisToro-Uribe, SaidIbáñez, ElenaDecker, Eric A.Villamizar-Jaimes, Arley RenéLópez-Giraldo, LuisCocoaPolyphenolsSolid–liquid kinetic extractionAntioxidantsThis article belongs to the Special Issue Antioxidants in Cocoa.Considering the increasing interest in the incorporation of natural antioxidants in enriched foods, this work aimed to establish a food-grade and suitable procedure for the recovery of polyphenols from cocoa beans avoiding the degreasing process. The results showed that ultrasound for 30 min with particle sample size < 0.18 mm changed the microstructure of the cell, thus increasing the diffusion pathway of polyphenols and avoiding the degreasing process. The effect of temperature, pH, and concentration of ethanol and solute on the extraction of polyphenols was evaluated. Through a 24 full factorial design, a maximum recovery of 122.34 ± 2.35 mg GAE/g, 88.87 ± 0.78 mg ECE/g, and 62.57 ± 3.37 mg ECE/g cocoa beans, for total concentration of polyphenols (TP), flavonoids (TF), and flavan-3-ols (TF3), respectively, was obtained. Based on mathematical models, the kinetics of the solid–liquid extraction process indicates a maximum equilibrium time of 45 min. Analysis by HPLC-DAD-ESI-MS/MS showed that our process allowed a high amount of methylxanthines (10.43 mg/g), catechins (7.92 mg/g), and procyanidins (34.0 mg/g) with a degree of polymerization >7, as well as high antioxidant activity determined by Oxygen Radical Absorbance Capacity (1149.85 ± 25.10 µMTrolox eq/g) and radical scavenging activity (DPPH•, 120.60 ± 0.50 µM Trolox eq/g). Overall, the recovery method made possible increases of 59.7% and 12.8% in cocoa polyphenols content and extraction yield, respectively. This study showed an effective, suitable and cost-effective process for the extraction of bioactive compounds from cocoa beans without degreasing.This research was funded by Universidad Industrial de Santander, grant number 2511. In addition, to Department of Science, Technology and Innovation (COLCIENCIAS-Colombia), grant number 567 of 2012.Peer reviewedMultidisciplinary Digital Publishing InstituteUniversidad Industrial de Santander (Colombia)Colciencias (Colombia)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/212511reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/antiox9050364Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2125112026-05-22T06:33:51Z
dc.title.none.fl_str_mv Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
title Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
spellingShingle Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
Toro-Uribe, Said
Cocoa
Polyphenols
Solid–liquid kinetic extraction
Antioxidants
title_short Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
title_full Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
title_fullStr Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
title_full_unstemmed Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
title_sort Food-safe process for high recovery of flavonoids from cocoa beans: Antioxidant and HPLC-DAD-ESI-MS/MS analysis
dc.creator.none.fl_str_mv Toro-Uribe, Said
Ibáñez, Elena
Decker, Eric A.
Villamizar-Jaimes, Arley René
López-Giraldo, Luis
author Toro-Uribe, Said
author_facet Toro-Uribe, Said
Ibáñez, Elena
Decker, Eric A.
Villamizar-Jaimes, Arley René
López-Giraldo, Luis
author_role author
author2 Ibáñez, Elena
Decker, Eric A.
Villamizar-Jaimes, Arley René
López-Giraldo, Luis
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Industrial de Santander (Colombia)
Colciencias (Colombia)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cocoa
Polyphenols
Solid–liquid kinetic extraction
Antioxidants
topic Cocoa
Polyphenols
Solid–liquid kinetic extraction
Antioxidants
description This article belongs to the Special Issue Antioxidants in Cocoa.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/212511
url http://hdl.handle.net/10261/212511
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/antiox9050364
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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