Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses

In wound healing, tissue growth, and certain cancers, the epithelial or the endothelial monolayer sheet expands. Within the expanding monolayer sheet, migration of the individual cell is strongly guided by physical forces imposed by adjacent cells. This process is called plithotaxis and was discover...

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Detalles Bibliográficos
Autores: Tambe, Dhananjay, Croutelle, Ugo, Trepat Guixer, Xavier, Park, Chan Young, Kim, Jae Hun, Millet, Emil J., Butler, James P., Fredberg, Jeffrey J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/96526
Acceso en línea:https://hdl.handle.net/2445/96526
Access Level:acceso abierto
Palabra clave:Migració cel·lular
Membranes cel·lulars
Cell migration
Cell membranes
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spelling Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stressesTambe, DhananjayCroutelle, UgoTrepat Guixer, XavierPark, Chan YoungKim, Jae HunMillet, Emil J.Butler, James P.Fredberg, Jeffrey J.Migració cel·lularMembranes cel·lularsCell migrationCell membranesIn wound healing, tissue growth, and certain cancers, the epithelial or the endothelial monolayer sheet expands. Within the expanding monolayer sheet, migration of the individual cell is strongly guided by physical forces imposed by adjacent cells. This process is called plithotaxis and was discovered using Monolayer Stress Microscopy (MSM). MSM rests upon certain simplifying assumptions, however, concerning boundary conditions, cell material properties and system dimensionality. To assess the validity of these assumptions and to quantify associated errors, here we report new analytical, numerical, and experimental investigations. For several commonly used experimental monolayer systems, the simplifying assumptions used previously lead to errors that are shown to be quite small. Out-of-plane components of displacement and traction fields can be safely neglected, and characteristic features of intercellular stresses that underlie plithotaxis remain largely unaffected. Taken together, these findings validate Monolayer Stress Microscopy within broad but well-defined limits of applicabilityPublic Library of Science (PLoS)2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/96526Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0055172PLoS One, 2013, vol. 8, num. 2, p. e52233http://dx.doi.org/10.1371/journal.pone.0055172info:eu-repo/grantAgreement/EC/FP7/242993cc-by (c) Tambe, D.T. et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/965262026-05-27T06:46:51Z
dc.title.none.fl_str_mv Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
title Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
spellingShingle Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
Tambe, Dhananjay
Migració cel·lular
Membranes cel·lulars
Cell migration
Cell membranes
title_short Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
title_full Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
title_fullStr Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
title_full_unstemmed Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
title_sort Monolayer stress microscopy: limitations, artifacts, and accuracy of recovered intercellular stresses
dc.creator.none.fl_str_mv Tambe, Dhananjay
Croutelle, Ugo
Trepat Guixer, Xavier
Park, Chan Young
Kim, Jae Hun
Millet, Emil J.
Butler, James P.
Fredberg, Jeffrey J.
author Tambe, Dhananjay
author_facet Tambe, Dhananjay
Croutelle, Ugo
Trepat Guixer, Xavier
Park, Chan Young
Kim, Jae Hun
Millet, Emil J.
Butler, James P.
Fredberg, Jeffrey J.
author_role author
author2 Croutelle, Ugo
Trepat Guixer, Xavier
Park, Chan Young
Kim, Jae Hun
Millet, Emil J.
Butler, James P.
Fredberg, Jeffrey J.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Migració cel·lular
Membranes cel·lulars
Cell migration
Cell membranes
topic Migració cel·lular
Membranes cel·lulars
Cell migration
Cell membranes
description In wound healing, tissue growth, and certain cancers, the epithelial or the endothelial monolayer sheet expands. Within the expanding monolayer sheet, migration of the individual cell is strongly guided by physical forces imposed by adjacent cells. This process is called plithotaxis and was discovered using Monolayer Stress Microscopy (MSM). MSM rests upon certain simplifying assumptions, however, concerning boundary conditions, cell material properties and system dimensionality. To assess the validity of these assumptions and to quantify associated errors, here we report new analytical, numerical, and experimental investigations. For several commonly used experimental monolayer systems, the simplifying assumptions used previously lead to errors that are shown to be quite small. Out-of-plane components of displacement and traction fields can be safely neglected, and characteristic features of intercellular stresses that underlie plithotaxis remain largely unaffected. Taken together, these findings validate Monolayer Stress Microscopy within broad but well-defined limits of applicability
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/96526
url https://hdl.handle.net/2445/96526
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0055172
PLoS One, 2013, vol. 8, num. 2, p. e52233
http://dx.doi.org/10.1371/journal.pone.0055172
info:eu-repo/grantAgreement/EC/FP7/242993
dc.rights.none.fl_str_mv cc-by (c) Tambe, D.T. et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Tambe, D.T. et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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