Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010

Our objective was to determine the genotype of the dengue virus type 2 (DENV-2) that circulated in the Amazon region of Peru between November 2010 and January 2011. We analyzed eight samples collected during dengue surveillance activities in the cities of Iquitos, Yurimaguas, Trujillo, Tarapoto and...

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Autores: Mamani, Enrique, Alvarez, Carlos, García M, María, Figueroa, Dana, Gatti, Milady, Guio, Heinner, Merino, Susy, Valencia, Pedro, Calampa, Carlos, Franco, Leticia, Cabezas, César
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:español
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/12842
Acceso en línea:http://hdl.handle.net/20.500.12105/12842
Access Level:acceso abierto
Palabra clave:Dengue Virus
Genotype
Peru
RNA, Viral
Serotyping
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spelling Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010Circulation of a different lineage of dengue virus serotype 2 American / Asian genotype in the Peruvian Amazon, 2010.Mamani, EnriqueAlvarez, CarlosGarcía M, MaríaFigueroa, DanaGatti, MiladyGuio, HeinnerMerino, SusyValencia, PedroCalampa, CarlosFranco, LeticiaCabezas, CésarDengue VirusGenotypePeruRNA, ViralSerotypingOur objective was to determine the genotype of the dengue virus type 2 (DENV-2) that circulated in the Amazon region of Peru between November 2010 and January 2011. We analyzed eight samples collected during dengue surveillance activities in the cities of Iquitos, Yurimaguas, Trujillo, Tarapoto and Lima between November 2010 and January 2011 that were sent to Insitituto Nacional de Salud. The viruses were isolated in C6/36 HT cell line. Viral RNA was extracted and the serotype (RT - PCR multiplex) and genotype (RT-Nested PCR of the region E/NS1) were determined. Finally, the E/ NS1 amplicons were sequenced and analyzed by phylogeny. The phylogenetic analysis revealed the introduction of a different lineage which entered in Peru by the end of 2010. These isolates found in Iquitos and other cities in Peru are closely related to DENV-2 isolates that circulated in Brazil during 2007 and 2008, associated with severe dengue cases and deaths. In conclusion, we detected the introduction of a different lineage of DENV-2 America / Asia genotype in Peru that could be associated with the presence of more severe cases.Instituto Nacional de Salud (Perú)Instituto Nacional de Salud (Perú)20212021-04-3020112011-03-0120112011-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/12842reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Españolspaopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/128422026-06-12T12:43:37Z
dc.title.none.fl_str_mv Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
Circulation of a different lineage of dengue virus serotype 2 American / Asian genotype in the Peruvian Amazon, 2010.
title Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
spellingShingle Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
Mamani, Enrique
Dengue Virus
Genotype
Peru
RNA, Viral
Serotyping
title_short Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
title_full Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
title_fullStr Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
title_full_unstemmed Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
title_sort Circulación de un linaje diferente del virus dengue 2 genotipo América / Asia en la región amazónica de Perú, 2010
dc.creator.none.fl_str_mv Mamani, Enrique
Alvarez, Carlos
García M, María
Figueroa, Dana
Gatti, Milady
Guio, Heinner
Merino, Susy
Valencia, Pedro
Calampa, Carlos
Franco, Leticia
Cabezas, César
author Mamani, Enrique
author_facet Mamani, Enrique
Alvarez, Carlos
García M, María
Figueroa, Dana
Gatti, Milady
Guio, Heinner
Merino, Susy
Valencia, Pedro
Calampa, Carlos
Franco, Leticia
Cabezas, César
author_role author
author2 Alvarez, Carlos
García M, María
Figueroa, Dana
Gatti, Milady
Guio, Heinner
Merino, Susy
Valencia, Pedro
Calampa, Carlos
Franco, Leticia
Cabezas, César
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Nacional de Salud (Perú)
dc.subject.none.fl_str_mv Dengue Virus
Genotype
Peru
RNA, Viral
Serotyping
topic Dengue Virus
Genotype
Peru
RNA, Viral
Serotyping
description Our objective was to determine the genotype of the dengue virus type 2 (DENV-2) that circulated in the Amazon region of Peru between November 2010 and January 2011. We analyzed eight samples collected during dengue surveillance activities in the cities of Iquitos, Yurimaguas, Trujillo, Tarapoto and Lima between November 2010 and January 2011 that were sent to Insitituto Nacional de Salud. The viruses were isolated in C6/36 HT cell line. Viral RNA was extracted and the serotype (RT - PCR multiplex) and genotype (RT-Nested PCR of the region E/NS1) were determined. Finally, the E/ NS1 amplicons were sequenced and analyzed by phylogeny. The phylogenetic analysis revealed the introduction of a different lineage which entered in Peru by the end of 2010. These isolates found in Iquitos and other cities in Peru are closely related to DENV-2 isolates that circulated in Brazil during 2007 and 2008, associated with severe dengue cases and deaths. In conclusion, we detected the introduction of a different lineage of DENV-2 America / Asia genotype in Peru that could be associated with the presence of more severe cases.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-03-01
2011
2011-03-01
2021
2021-04-30
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/12842
url http://hdl.handle.net/20.500.12105/12842
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Nacional de Salud (Perú)
publisher.none.fl_str_mv Instituto Nacional de Salud (Perú)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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