A molecular approach to the study of birds’ DNA in bats faeces

amplify prey's DNA. In this study we evaluated and compared the efficiency of two total DNA extraction methods and five primer pairs for the molecular identification of birds from scats, in particular from the giant noctule bat (Nyctalus lasiopterus). A modified DNA stool Mini Kit of Qiagen was...

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Detalles Bibliográficos
Autores: Pastor-Beviá, David, Ibáñez, Carlos, García-Mudarra, Juan L., Juste, Javier
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/111324
Acceso en línea:http://hdl.handle.net/10261/111324
Access Level:acceso abierto
Palabra clave:Amplification success
Bird DNA
Conservation
DNA extraction
Faeces
Gian noctule bat
primer design
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spelling A molecular approach to the study of birds’ DNA in bats faecesPastor-Beviá, DavidIbáñez, CarlosGarcía-Mudarra, Juan L.Juste, JavierAmplification successBird DNAConservationDNA extractionFaecesGian noctule batprimer designamplify prey's DNA. In this study we evaluated and compared the efficiency of two total DNA extraction methods and five primer pairs for the molecular identification of birds from scats, in particular from the giant noctule bat (Nyctalus lasiopterus). A modified DNA stool Mini Kit of Qiagen was tested against a modified silica method with a guanidinium thiocianate (GuSCN) applied after freezing and pulverizing the samples. We also checked two published vertebrate- and bird-generalist primer pairs and three bird-specific primer pairs designed by us (two pairs targeting the cytochrome b and one the cytochrome oxidase subunit I genes) that amplified shorter DNA fragments. The results show that pulverizing the scat remains before extraction was a very important step, presumably facilitating access to the well preserved DNA located inside the rachis of the feathers. The combination of our bird-specific designed primers showed a higher amplification rate than the generalist primers and allowed successful bird identification from the feathers excreted by the giant noctule bat in all the scat samples analyzed, independent of the preservation method used (dried and frozen). These methodological improvements will allow not only the study of the avian diet composition of the enigmatic giant noctule, but the extension of this methodology to other bird predators such as raptors.Peer reviewedPolish Academy of SciencesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/111324reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3161/150811014X687378Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1113242026-05-22T06:33:51Z
dc.title.none.fl_str_mv A molecular approach to the study of birds’ DNA in bats faeces
title A molecular approach to the study of birds’ DNA in bats faeces
spellingShingle A molecular approach to the study of birds’ DNA in bats faeces
Pastor-Beviá, David
Amplification success
Bird DNA
Conservation
DNA extraction
Faeces
Gian noctule bat
primer design
title_short A molecular approach to the study of birds’ DNA in bats faeces
title_full A molecular approach to the study of birds’ DNA in bats faeces
title_fullStr A molecular approach to the study of birds’ DNA in bats faeces
title_full_unstemmed A molecular approach to the study of birds’ DNA in bats faeces
title_sort A molecular approach to the study of birds’ DNA in bats faeces
dc.creator.none.fl_str_mv Pastor-Beviá, David
Ibáñez, Carlos
García-Mudarra, Juan L.
Juste, Javier
author Pastor-Beviá, David
author_facet Pastor-Beviá, David
Ibáñez, Carlos
García-Mudarra, Juan L.
Juste, Javier
author_role author
author2 Ibáñez, Carlos
García-Mudarra, Juan L.
Juste, Javier
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Amplification success
Bird DNA
Conservation
DNA extraction
Faeces
Gian noctule bat
primer design
topic Amplification success
Bird DNA
Conservation
DNA extraction
Faeces
Gian noctule bat
primer design
description amplify prey's DNA. In this study we evaluated and compared the efficiency of two total DNA extraction methods and five primer pairs for the molecular identification of birds from scats, in particular from the giant noctule bat (Nyctalus lasiopterus). A modified DNA stool Mini Kit of Qiagen was tested against a modified silica method with a guanidinium thiocianate (GuSCN) applied after freezing and pulverizing the samples. We also checked two published vertebrate- and bird-generalist primer pairs and three bird-specific primer pairs designed by us (two pairs targeting the cytochrome b and one the cytochrome oxidase subunit I genes) that amplified shorter DNA fragments. The results show that pulverizing the scat remains before extraction was a very important step, presumably facilitating access to the well preserved DNA located inside the rachis of the feathers. The combination of our bird-specific designed primers showed a higher amplification rate than the generalist primers and allowed successful bird identification from the feathers excreted by the giant noctule bat in all the scat samples analyzed, independent of the preservation method used (dried and frozen). These methodological improvements will allow not only the study of the avian diet composition of the enigmatic giant noctule, but the extension of this methodology to other bird predators such as raptors.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/111324
url http://hdl.handle.net/10261/111324
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3161/150811014X687378
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Polish Academy of Sciences
publisher.none.fl_str_mv Polish Academy of Sciences
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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