The Domestic Environment and the Lung Mycobiome

This study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from t...

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Autores: Rubio-Portillo, E, Orts, D, Llorca, E, Fernandez, C, Anton, J, Ferrer, C, Galvez, B, Esteban, V, Revelles, E, Perez-Martin, C, Gomez-Imbernon, E, Adsuar, J, Piqueras, P, Amat, B, Franco, J, Colom, MF
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
Repositorio:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
OAI Identifier:oai:isabial.fundanetsuite.com:p6519
Acceso en línea:https://isabial.portalinvestigacion.com/publicaciones6519
Access Level:acceso abierto
Palabra clave:fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
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spelling The Domestic Environment and the Lung MycobiomeRubio-Portillo, EOrts, DLlorca, EFernandez, CAnton, JFerrer, CGalvez, BEsteban, VRevelles, EPerez-Martin, CGomez-Imbernon, EAdsuar, JPiqueras, PAmat, BFranco, JColom, MFfungimycobiomelower respiratory tracthouse dustmycobiotabronco-alveolar lavageThis study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from the houses (ENV) of 20 of them (44.4%). Additionally, five bronchoscopes (BS) were also analyzed and negative controls were included for every procedure. All samples were processed for DNA extraction and cultures, which were performed in Sabouraud Dextrose and Potato Dextrose Agar. The fungal Internal Transcribed Spacer (ITS2) was sequenced by the Solexa/Illumina system and sequences were analyzed by QIIME 1.8.0 and compared with the UNITE Database for identification. The similarity between the two fungal communities (BAL and ENV) for a specific patient was assessed via the percentage of coincidence in the detection of specific operational taxonomic units (OTUs), and about 75% of co-occurrence was detected between the mycobiome of the LRT and the houses. Cultures confirmed the presence of the core mycobiome species. However, the low rate of isolation from BAL suggests that most of its mycobiome corresponds to non-culturable cells. This likely depends on the patient's immune system activity and inflammatory status.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://isabial.portalinvestigacion.com/publicaciones6519MicroorganismsISSN: 20762607reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicanteinstname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)Inglésinfo:eu-repo/semantics/openAccessoai:isabial.fundanetsuite.com:p65192026-06-12T10:20:37Z
dc.title.none.fl_str_mv The Domestic Environment and the Lung Mycobiome
title The Domestic Environment and the Lung Mycobiome
spellingShingle The Domestic Environment and the Lung Mycobiome
Rubio-Portillo, E
fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
title_short The Domestic Environment and the Lung Mycobiome
title_full The Domestic Environment and the Lung Mycobiome
title_fullStr The Domestic Environment and the Lung Mycobiome
title_full_unstemmed The Domestic Environment and the Lung Mycobiome
title_sort The Domestic Environment and the Lung Mycobiome
dc.creator.none.fl_str_mv Rubio-Portillo, E
Orts, D
Llorca, E
Fernandez, C
Anton, J
Ferrer, C
Galvez, B
Esteban, V
Revelles, E
Perez-Martin, C
Gomez-Imbernon, E
Adsuar, J
Piqueras, P
Amat, B
Franco, J
Colom, MF
author Rubio-Portillo, E
author_facet Rubio-Portillo, E
Orts, D
Llorca, E
Fernandez, C
Anton, J
Ferrer, C
Galvez, B
Esteban, V
Revelles, E
Perez-Martin, C
Gomez-Imbernon, E
Adsuar, J
Piqueras, P
Amat, B
Franco, J
Colom, MF
author_role author
author2 Orts, D
Llorca, E
Fernandez, C
Anton, J
Ferrer, C
Galvez, B
Esteban, V
Revelles, E
Perez-Martin, C
Gomez-Imbernon, E
Adsuar, J
Piqueras, P
Amat, B
Franco, J
Colom, MF
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
topic fungi
mycobiome
lower respiratory tract
house dust
mycobiota
bronco-alveolar lavage
description This study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from the houses (ENV) of 20 of them (44.4%). Additionally, five bronchoscopes (BS) were also analyzed and negative controls were included for every procedure. All samples were processed for DNA extraction and cultures, which were performed in Sabouraud Dextrose and Potato Dextrose Agar. The fungal Internal Transcribed Spacer (ITS2) was sequenced by the Solexa/Illumina system and sequences were analyzed by QIIME 1.8.0 and compared with the UNITE Database for identification. The similarity between the two fungal communities (BAL and ENV) for a specific patient was assessed via the percentage of coincidence in the detection of specific operational taxonomic units (OTUs), and about 75% of co-occurrence was detected between the mycobiome of the LRT and the houses. Cultures confirmed the presence of the core mycobiome species. However, the low rate of isolation from BAL suggests that most of its mycobiome corresponds to non-culturable cells. This likely depends on the patient's immune system activity and inflammatory status.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://isabial.portalinvestigacion.com/publicaciones6519
url https://isabial.portalinvestigacion.com/publicaciones6519
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Microorganisms
ISSN: 20762607
reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
instname_str Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
reponame_str r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
collection r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
repository.name.fl_str_mv
repository.mail.fl_str_mv
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